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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 22 — Oct. 26, 2009
  • pp: 20401–20414

Optical Scatter Imaging with a digital micromirror device

Jing-Yi Zheng, Robert M. Pasternack, and Nada N. Boustany  »View Author Affiliations

Optics Express, Vol. 17, Issue 22, pp. 20401-20414 (2009)

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We had developed Optical Scatter Imaging (OSI) as a method which combines light scattering spectroscopy with microscopic imaging to probe local particle size in situ. Using a variable diameter iris as a Fourier spatial filter, the technique consisted of collecting images that encoded the intensity ratio of wide-to-narrow angle scatter at each pixel in the full field of view. In this paper, we replace the variable diameter Fourier filter with a digital micromirror device (DMD) to extend our assessment of morphology to the characterization of particle shape and orientation. We describe our setup in detail and demonstrate how to eliminate aberrations associated with the placement of the DMD in a conjugate Fourier plane of our microscopic imaging system. Using bacteria and polystyrene spheres, we show how this system can be used to assess particle aspect ratio even when imaged at low resolution. We also show the feasibility of detecting alterations in organelle aspect ratio in situ within living cells. This improved OSI system could be further developed to automate morphological quantification and sorting of non-spherical particles in situ.

© 2009 OSA

OCIS Codes
(070.1170) Fourier optics and signal processing : Analog optical signal processing
(070.6110) Fourier optics and signal processing : Spatial filtering
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(070.6120) Fourier optics and signal processing : Spatial light modulators

ToC Category:
Fiber Optics

Original Manuscript: August 20, 2009
Revised Manuscript: September 29, 2009
Manuscript Accepted: October 4, 2009
Published: October 23, 2009

Virtual Issues
Vol. 4, Iss. 12 Virtual Journal for Biomedical Optics

Jing-Yi Zheng, Robert M. Pasternack, and Nada N. Boustany, "Optical Scatter Imaging with a digital micromirror device," Opt. Express 17, 20401-20414 (2009)

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