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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 25 — Dec. 7, 2009
  • pp: 23104–23113

Intensity sensitivity of gold nanostructures and its application for high-throughput biosensing

Kuang-Li Lee, Shu-Han Wu, and Pei-Kuen Wei  »View Author Affiliations

Optics Express, Vol. 17, Issue 25, pp. 23104-23113 (2009)

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A new microarray for dynamical studies of surface biomolecular interactions without fluorescent labeling is proposed. We employed gold nanostructures to excite surface plasmons on the microarray surface and detected the intensity changes in the extraordinary transmission. The calculation and measurement results indicate that the nanoslit array has an intensity sensitivity much higher than the nanohole array due to its narrower resonant bandwidth. In addition, the sensitivity is increased as the slit width decreases. For 35 nm slit width, the intensity sensitivity reaches to ~4000%/RIU, two times larger than the slit width larger than 150 nm. Using the intensity changes, we demonstrate a 10 × 10 microarray for real-time measurements of antigen-antibody and DNA-DNA interactions.

© 2009 OSA

OCIS Codes
(240.6680) Optics at surfaces : Surface plasmons
(260.3910) Physical optics : Metal optics
(280.4788) Remote sensing and sensors : Optical sensing and sensors
(050.6624) Diffraction and gratings : Subwavelength structures

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Original Manuscript: October 8, 2009
Revised Manuscript: November 20, 2009
Manuscript Accepted: November 20, 2009
Published: December 2, 2009

Virtual Issues
Vol. 5, Iss. 1 Virtual Journal for Biomedical Optics

Kuang-Li Lee, Shu-Han Wu, and Pei-Kuen Wei, "Intensity sensitivity of gold nanostructures and its application for high-throughput biosensing," Opt. Express 17, 23104-23113 (2009)

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