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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 26 — Dec. 21, 2009
  • pp: 24162–24174

Spectroscopic measurements with dispersion encoded full range frequency domain optical coherence tomography in single- and multilayered non–scattering phantoms

B. Hermann, B. Hofer, C. Meier, and W. Drexler  »View Author Affiliations

Optics Express, Vol. 17, Issue 26, pp. 24162-24174 (2009)

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Depth resolved measurements of absorption profiles in the wavelength range of 800 nm with a bandwidth of 120 nm are demonstrated using high speed spectroscopic frequency domain OCT (SOCT) and a full range reconstruction algorithm (dispersion encoded full range, DEFR). The feasibility of the algorithm for SOCT is tested in simulation and experiment. With proper calibration, SOCT with DEFR is able to extract absolute, depth resolved absorption profiles over the whole wavelength range at once without the need of tuning and performing measurements at single wavelengths sequentially. The superior acquisition speed and better phase stability in frequency domain as compared to time domain results in a better reproducibility and practicability for spectroscopic measurements. In addition, high acquisition speed in excess of 20 kHz allows to measure absorption dynamics with 50 µs time resolution, which might be useful for the investigation of pharmacokinetics or pharmacodynamics. SOCT of ~600 µm thick single- and multilayered, non–scattering phantoms with varying absorption in the range of 5–80 cm-1, equivalent to blood absorption in capillaries, is presented. SOCT measurements are compared with those using a spectrometer in transmission mode. For Indocyanine Green (ICG), dynamic absorption measurements are demonstrated.

© 2009 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(260.2030) Physical optics : Dispersion

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 8, 2009
Revised Manuscript: November 15, 2009
Manuscript Accepted: December 12, 2009
Published: December 17, 2009

Virtual Issues
Vol. 5, Iss. 1 Virtual Journal for Biomedical Optics

Boris Hermann, Bernd Hofer, Christoph Meier, and Wolfgang Drexler, "Spectroscopic measurements with dispersion encoded full range frequency domain optical coherence tomography in single- and multilayered non- scattering phantoms," Opt. Express 17, 24162-24174 (2009)

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