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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 26 — Dec. 21, 2009
  • pp: 24403–24413

An optical imaging method to monitor stem cell migration in a model of immune-mediated arthritis

Elizabeth J. Sutton, Sophie E. Boddington, Alexander J. Nedopil, Tobias D. Henning, Stavros G. Demos, Rick Baehner, Barbara Sennino, Ying Lu, and Heike E. Daldrup-Link  »View Author Affiliations

Optics Express, Vol. 17, Issue 26, pp. 24403-24413 (2009)

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The objective of this work is to establish an optical imaging technique that would enable monitoring of the integration of mesenchymal stem cells (MSC) in arthritic joints. Our approach is based on first developing a labeling technique of MSC with the fluorescent dye DiD followed by tracking the cell migration kinetics from the spatial distribution of the DiD fluorescence in optical images (OI). The experimental approach involves first the in vitro OI of MSC labeled with DiD accompanied by fluorescence microscopy measurements to establish localization of the signal within the cells. Thereafter, DiD-labeled MSC were injected into polyarthritic, athymic rats and the signal localization within the experimental animals was monitored over several days. The experimental results indicate that DiD integrated into the cell membrane. DiD-labeled MSC localization in the arthritic ankle joints was observed with OI indicating that this method can be applied to monitor MSC in arthritic joints.

© 2009 OSA

OCIS Codes
(170.1610) Medical optics and biotechnology : Clinical applications
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: October 8, 2009
Revised Manuscript: November 16, 2009
Manuscript Accepted: November 21, 2009
Published: December 18, 2009

Virtual Issues
Vol. 5, Iss. 1 Virtual Journal for Biomedical Optics

Elizabeth J. Sutton, Sophie E. Boddington, Alexander J. Nedopil, Tobias D. Henning, Stavros G. Demos, Rick Baehner, Barbara Sennino, Ying Lu, and Heike E. Daldrup-Link, "An optical imaging method to monitor stem cell migration in a model of immune-mediated arthritis," Opt. Express 17, 24403-24413 (2009)

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