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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 4 — Feb. 16, 2009
  • pp: 2579–2585

Diffraction Phase Cytometry: blood on a CD-ROM

Mustafa Mir, Zhuo Wang, Krishnarao Tangella, and Gabriel Popescu  »View Author Affiliations

Optics Express, Vol. 17, Issue 4, pp. 2579-2585 (2009)

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We demonstrate Diffraction Phase Cytometry (DPC) as a single shot, full-field, high throughput quantitative phase imaging modality, dedicated to analyzing whole blood smears. Utilizing a commercial CD as a sample substrate, along with dynamic spatial filtering via a liquid crystal spatial light modulator, we have developed a compact instrument capable of making quantitative, physiologically relevant measurements. To illustrate the ability of the system to function as a highly sensitive cytometer we imaged a large number (N=1,537) of live human erythrocytes in whole blood without preparation. We retrieved a comprehensive set of geometrical parameters including cell volume and surface area, which are not directly available using existing cytometers. Furthermore, we retrieved the minimum cylindrical diameter, through which red blood cells can pass, and deliver oxygen. These initial results prove the concept for an inexpensive lab-on-a-chip blood screening device.

© 2009 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.3170) Microscopy : Interference microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: December 17, 2008
Revised Manuscript: January 30, 2009
Manuscript Accepted: February 1, 2009
Published: February 6, 2009

Virtual Issues
Vol. 4, Iss. 4 Virtual Journal for Biomedical Optics

Mustafa Mir, Zhuo Wang, Krishnarao Tangella, and Gabriel Popescu, "Diffraction Phase Cytometry: blood on a CD-ROM," Opt. Express 17, 2579-2585 (2009)

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