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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 4 — Feb. 16, 2009
  • pp: 2805–2817

Diffuse reflectance spectroscopy detects increased hemoglobin concentration and decreased oxygenation during colon carcinogenesis from normal to malignant tumors

Hsing-Wen Wang, Jeng-Kai Jiang, Chi-Hung Lin, Jen-Kou Lin, Guang-Jie Huang, and Jia-Sin Yu  »View Author Affiliations

Optics Express, Vol. 17, Issue 4, pp. 2805-2817 (2009)

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Blood content and tumor oxygen level are important biomarkers and prognostic indicators in patients with colorectal cancer (CRC). However, noninvasive measurements of both quantities in human colon are limited. In this study, we extracted the total hemoglobin concentration (THC) and oxygen saturation (StO2) of normal, premalignant, and malignant colonic tissues in 27 patients using a diffuse reflectance instrument and algorithms based on the diffusion equation. The mean±standard error of THC and StO2 from all normal sites (n=26) is 93.4±17.1μM and 67.2±3.7%, respectively. THC increased to 136.9±23.8μM and 153.8±38.6μM and StO2 decreased to 51.3±7.0% and 26.4±6.1% for premalignant and malignant tissues, respectively. The disease-to-normal THC ratios are 3.2±1.1 and 4.4±1.9 and the disease-to-normal StO2 ratios are 0.7±0.1 and 0.5±0.1 for premalignant and malignant tissues, respectively. These results demonstrate the feasibility of a robust optical method to assess colon THC and StO2 at all stages of carcinogenesis in vivo so that the angiogenesis and hypoxia of the disease and the therapeutic role can be studied in CRC patients.

© 2009 Optical Society of America

OCIS Codes
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: December 2, 2008
Revised Manuscript: February 3, 2009
Manuscript Accepted: February 9, 2009
Published: February 11, 2009

Virtual Issues
Vol. 4, Iss. 4 Virtual Journal for Biomedical Optics

Hsing-Wen Wang, Jeng-Kai Jiang, Chi-Hung Lin, Jen-Kou Lin, Guang-Jie Huang, and Jia-Sin Yu, "Diffuse reflectance spectroscopy detects increased hemoglobin concentration and decreased oxygenation during colon carcinogenesis from normal to malignant tumors," Opt. Express 17, 2805-2817 (2009)

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