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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 4 — Feb. 16, 2009
  • pp: 2984–2996

Optimally chirped multimodal CARS microscopy based on a single Ti:sapphire oscillator

Adrian F. Pegoraro, Andrew Ridsdale, Douglas J. Moffatt, Yiwei Jia, John Paul Pezacki, and Albert Stolow  »View Author Affiliations


Optics Express, Vol. 17, Issue 4, pp. 2984-2996 (2009)
http://dx.doi.org/10.1364/OE.17.002984


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Abstract

We demonstrate high performance coherent anti-Stokes Raman scattering (CARS) microscopy of live cells and tissues with user-variable spectral resolution and broad Raman tunability (2500 - 4100 cm-1), using a femtosecond Ti:Sapphire pump and photonic crystal fiber output for the broadband synchronized Stokes pulse. Spectral chirp of the fs laser pulses was a user-variable parameter for optimization in a spectral focussing implementation of multimodal CARS microscopy. High signal-to-noise, high contrast multimodal imaging of live cells and tissues was achieved with pixel dwell times of 2-8 μs and low laser powers (< 30 mW total).

© 2009 Optical Society of America

OCIS Codes
(170.5810) Medical optics and biotechnology : Scanning microscopy
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: January 2, 2009
Revised Manuscript: February 1, 2009
Manuscript Accepted: February 1, 2009
Published: February 12, 2009

Virtual Issues
Vol. 4, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Adrian F. Pegoraro, Andrew Ridsdale, Douglas J. Moffatt, Yiwei Jia, John Paul Pezacki, and Albert Stolow, "Optimally chirped multimodal CARS microscopy based on a single Ti:sapphire oscillator," Opt. Express 17, 2984-2996 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-4-2984


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