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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 6 — Mar. 16, 2009
  • pp: 4868–4878

Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging

Paolo Matteini, Fulvio Ratto, Francesca Rossi, Riccardo Cicchi, Chiara Stringari, Dimitrios Kapsokalyvas, Francesco S. Pavone, and Roberto Pini  »View Author Affiliations

Optics Express, Vol. 17, Issue 6, pp. 4868-4878 (2009)

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The loss of organization of the corneal collagen lattice induced by photothermal effects was analyzed by using second-harmonic generation (SHG) imaging. Porcine cornea samples were treated with low-power laser irradiation in order to get localized areas of tissue disorganization. The disorder induced within the irradiated area of corneal stroma was quantified by means of Discrete Fourier Transform, auto-correlation and entropy analyses of the SHG images. Polarization modulated SHG measurements allowed to probe the changes in the structural anisotropy of sub-micron hierarchical levels of the stromal collagen. Our results emphasize the great potential of the SHG imaging to detect subtle modifications in the collagen assembly. The proposed analytical methods may be used to track several genetic, pathologic, accidental or surgical-induced disorder states of biological tissues.

© 2009 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(350.5340) Other areas of optics : Photothermal effects
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: October 24, 2008
Revised Manuscript: November 26, 2008
Manuscript Accepted: November 27, 2008
Published: March 13, 2009

Virtual Issues
Vol. 4, Iss. 5 Virtual Journal for Biomedical Optics

Paolo Matteini, Fulvio Ratto, Francesca Rossi, Riccardo Cicchi, Chiara Stringari, Dimitrios Kapsokalyvas, Francesco S. Pavone, and Roberto Pini, "Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging," Opt. Express 17, 4868-4878 (2009)

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