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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 8 — Apr. 13, 2009
  • pp: 6476–6486

Autofocusing and edge detection schemes in cell volume measurements with quantitative phase microscopy

Seungrag Lee, Ji Yong Lee, Wenzhong Yang, and Dug Young Kim  »View Author Affiliations


Optics Express, Vol. 17, Issue 8, pp. 6476-6486 (2009)
http://dx.doi.org/10.1364/OE.17.006476


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Abstract

We have proposed and demonstrated a very sensitive volume measurement scheme for a live cell with a quantitative phase microscopy (QPM) utilizing auto-focusing and numerical edge detection schemes. An auto-focusing technique with two different focus measures is applied to find the focus dependent errors in our live cell volume measurement system. The volume of a polystyrene bead sample with 3 μm diameter has been measured for the validity test of our proposed method. We have shown that a small displacement of an object from its focusing position can cause a large volume error. A numerical edge detection technique is also used to accurately resolve the boundary between a cell and its suspension medium. We have applied this method to effectively suppress errors by the surrounding medium of a single red blood cell (RBC).

© 2009 Optical Society of America

OCIS Codes
(100.2000) Image processing : Digital image processing
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring

ToC Category:
Instrumentation, Measurement, and Metrology

History
Original Manuscript: December 22, 2008
Revised Manuscript: March 12, 2009
Manuscript Accepted: March 27, 2009
Published: April 3, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Seungrag Lee, Ji Yong Lee, Wenzhong Yang, and Dug Young Kim, "Autofocusing and edge detection schemes in cell volume measurements with quantitative phase microscopy," Opt. Express 17, 6476-6486 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-8-6476


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