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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 8 — Apr. 13, 2009
  • pp: 6710–6720

Holographic soft X-ray omni-microscopy of biological specimens

Erik Guehrs, Christian M. Günther, René Könnecke, Bastian Pfau, and Stefan Eisebitt  »View Author Affiliations

Optics Express, Vol. 17, Issue 8, pp. 6710-6720 (2009)

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Mask-based digital soft X-ray Fourier transform holography using coherent synchrotron radiation is applied to image diatoms and freeze-dried 3T3 fibroblast cells. At soft X-ray wavelengths between 4 nm and 8 nm we achieve spatial resolutions down to 450 nm over a 33.5 μm field of view. As holography records amplitude and phase of the scattered wavefield, images with different contrast mechanisms can be generated a posteriori by numerical processing of the holographic data (“omni-microscopy”). We present Zernike phase contrast, Schlieren phase contrast, differential interference and gradient contrast images based on amplitude and phase information. We discuss the potential of this approach for destructive single-shot imaging beyond dose limitations of biological samples at coherent femtosecond-pulsed X-ray sources.

© 2009 Optical Society of America

OCIS Codes
(110.7440) Imaging systems : X-ray imaging
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(090.1995) Holography : Digital holography

ToC Category:
Imaging Systems

Original Manuscript: February 4, 2009
Revised Manuscript: March 19, 2009
Manuscript Accepted: March 22, 2009
Published: April 8, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Erik Guehrs, Christian M. Günther, René Könnecke, Bastian Pfau, and Stefan Eisebitt, "Holographic soft X-ray omni-microscopy of biological specimens," Opt. Express 17, 6710-6720 (2009)

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