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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 9 — Apr. 27, 2009
  • pp: 7339–7347

On illumination schemes for wide-field CARS microscopy

I. Toytman, D. Simanovskii, and D. Palanker  »View Author Affiliations


Optics Express, Vol. 17, Issue 9, pp. 7339-7347 (2009)
http://dx.doi.org/10.1364/OE.17.007339


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Abstract

New system for a wide-field CARS microscopy is demonstrated, including two schemes of non-phase-matching illumination. Several advantages including high Stokes pulse energy, pulse-to-pulse stability and inherent synchronization between pump and Stokes pulses were brought by use of methane-filled Raman converter. Spatial resolution of the system with axially symmetric illumination, 0.5μm, was found to correspond to diffraction limit of the imaging objective. Selective sensitivity to lipid-rich myelin sheaths in the nerve tissue has been demonstrated and confirmed by comparison with histological samples stained with myelin-specific dye. Single-shot imaging capability of the system has been demonstrated with a speckling-free illumination on a monolayer of 3μm polystyrene beads.

© 2009 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(190.5650) Nonlinear optics : Raman effect
(190.5890) Nonlinear optics : Scattering, stimulated

ToC Category:
Microscopy

History
Original Manuscript: January 21, 2009
Revised Manuscript: March 5, 2009
Manuscript Accepted: April 7, 2009
Published: April 20, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Citation
I. Toytman, D. Simanovskii, and D. Palanker, "On illumination schemes for wide-field CARS microscopy," Opt. Express 17, 7339-7347 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-9-7339


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References

  1. E. Kohen and J. G. Hirschberg, Cell Structure and Function by Microspectrofluorometry (Academic, San Diego, Calif., 1989).
  2. W. C. W. Chan and S. Nie, "Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection," Science 281, 2016-2018 (1998). [CrossRef] [PubMed]
  3. D. Dosev, M. Nichkova, M. Liu, B. Guo, G. Liu, Y. Xia, B. D. Hammock, and I. M. Kennedy, "Application of fluorescent Eu:Gd2O3 nanoparticles to the visualization of protein micropatterns," Proc. SPIE 5699, 473-481 (2005). [CrossRef]
  4. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical Coherence Tomography," Science 254, 1178-1181 (1991). [CrossRef] [PubMed]
  5. J.-X. Cheng, Y. K. Jia, G. Zheng, and X. S. Xie, "Laser-Scanning Coherent Anti-Stokes Raman Scattering Microscopy and Applications to Cell Biology," Biophys. J. 83, 502-509 (2002). [CrossRef] [PubMed]
  6. E. O. Potma and X. S. Xie, "Detection of single lipid bilayers with coherent anti-Stokes Raman scattering (CARS) microscopy," J. Raman Spectrosc. 34, 642-650 (2003). [CrossRef]
  7. C. P. Pfeffer, B. R. Olsen, F. Ganikhanov, and F. Legare, "Multimodal nonlinear optical imaging of collagen arrays," J. Struct. Biol. 164, 140-145 (2008). [CrossRef] [PubMed]
  8. E. O. Potma, X. S. Xie, L. Muntean, J. Preusser, D. Jones, J. Ye, S. R. Leone, W. D. Hinsberg, and W. Schade, "Chemical Imaging of Photoresists with Coherent Anti-Stokes Raman Scattering (CARS) Microscopy," J. Phys. Chem. B 108, 1296-1301 (2004). [CrossRef]
  9. C. Heinrich, S. Bernet, and M. Ritsch-Marte, "Wide-field coherent anti-Stokes Raman scattering microscopy," Appl. Phys. Lett. 84, 816-818 (2004). [CrossRef]
  10. C. Heinrich, A. Hofer, A. Ritsch, C. Ciardi, S. Bernet, and M. Ritsch-Marte, "Selective imaging of saturated and unsaturated lipids by wide-field CARS-microscopy," Opt. Express 16, 2699-2708 (2008). [CrossRef] [PubMed]
  11. I. Toytman, K. Cohn, T. Smith, D. Simanovskii, and D. Palanker, "Wide-field coherent anti-Stokes Raman scattering microscopy with non-phase-matching illumination," Opt. Lett. 32, 1941-1943 (2007). [CrossRef] [PubMed]
  12. C. L. Evans and X. S. Xie, "Coherent Anti-Stokes Raman Scattering Microscopy: Chemically Selective Imaging for Biology and Medicine," Annu. Rev. Anal. Chem. 1, 883-909 (2008). [CrossRef]
  13. C. L. Evans, E. O. Potma, M. Puoris’haag, D. Cote, C. P. Lin, and X. S. Xie, "Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy," Proc. Nat. Acad. Sci. 102, 16807-16812 (2005). [CrossRef] [PubMed]
  14. H. Wang, Y. Fu, P. Zickmund, R. Shi, and J.-X. Cheng, "Coherent Anti-Stokes Raman Scattering Imaging of Axonal Myelin in Live Spinal Tissue," Biophys. J. 89, 581-591 (2005). [CrossRef] [PubMed]

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