OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 10 — May. 10, 2010
  • pp: 9840–9851

Label-free second harmonic generation holographic microscopy of biological specimens

Omid Masihzadeh, Philip Schlup, and Randy A. Bartels  »View Author Affiliations


Optics Express, Vol. 18, Issue 10, pp. 9840-9851 (2010)
http://dx.doi.org/10.1364/OE.18.009840


View Full Text Article

Enhanced HTML    Acrobat PDF (1653 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Second-order nonlinear holographic microscopy for highspeed, three-dimensional imaging is demonstrated. The use of harmonic generation allows image formation of endogenous features in biological samples such as muscle tissue. We have acquired holograms with acquisition times as short as 10 ms, limited by the switching speed of our shutter; frame rates of 100’s of Hz are expected to be possible. The samples are imaged with a Yb:KGW femtosecond laser oscillator, whose 1027 nm wavelength is well suited to minimize absorption and scattering. The low average power of the oscillator prevents damage to the sample.

© 2010 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(190.7110) Nonlinear optics : Ultrafast nonlinear optics
(090.1995) Holography : Digital holography

ToC Category:
Microscopy

History
Original Manuscript: April 8, 2010
Manuscript Accepted: April 23, 2010
Published: April 27, 2010

Virtual Issues
Vol. 5, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Omid Masihzadeh, Philip Schlup, and Randy A. Bartels, "Label-free second harmonic generation holographic microscopy of biological specimens," Opt. Express 18, 9840-9851 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-10-9840


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. M. Antkowiak, N. Callens, C. Yourassowsky, and F. Dubois, "Extended focused imaging of a microparticle field with digital holographic microscopy," Opt. Lett. 33, 1626-1628 (2008). [CrossRef] [PubMed]
  2. Y. Otsu, V. Bormuth, J. Wong, B. Mathieu, G. P. Dugue, A. Feltz, and S. Dieudonne, "Optical monitoring of neuronal activity at high frame rate with a digital random-access multiphoton (RAMP) microscope," J. Neurosci. Meth. 173, 259-270 (2008). [CrossRef]
  3. M. S. Amin, Y. Park, N. Lue, R. R. Dasari, K. Badizadegan, M. S. Feld, and G. Popescu, "Microrheology of red blood cell membranes using dynamic scattering microscopy," Opt. Express 15, 17001-17009 (2007). [CrossRef] [PubMed]
  4. W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, "Tomographic phase microscopy," Nat. Methods 4, 717-719 (2007). [CrossRef] [PubMed]
  5. N. Lue, W. Choi, G. Popescu, T. Ikeda, R. R. Dasari, K. Badizadegan, and M. S. Feld, "Quantitative phase imaging of live cells using fast Fourier phase microscopy," Appl. Opt. 46, 1836-1842 (2007). [CrossRef] [PubMed]
  6. V. Crepel, D. Aronov, I. Jorquera, A. Represa, Y. Ben Ari, and R. Cossart, "A parturition-associated nonsynaptic coherent activity pattern in the developing hippocampus," Neuron 54, 105-120 (2007). [CrossRef] [PubMed]
  7. O. Garaschuk, R. I. Milos, C. Grienberger, N. Marandi, H. Adelsberger, and A. Konnerth, "Optical monitoring of brain function in vivo: from neurons to networks," Pfl¨ugers Archiv European Journal of Physiology 453, 385-396 (2006). [CrossRef] [PubMed]
  8. T. A. Ooms, R. Lindken, and J. Westerweel, "Digital holographic microscopy applied to measurement of a flow in a T-shaped micromixer," Exp. Fluids 47, 941-955 (2009). [CrossRef]
  9. Y.-S. Choi and S.-J. Lee, "Three-dimensional volumetric measurement of red blood cell motion using digital holographic microscopy," Appl. Opt. 48, 2983-2990 (2009). [CrossRef] [PubMed]
  10. L. Sacconi, J. Mapelli, D. Gandolfi, J. Lotti, R.-P. O’Connor, E. D’Angelo, and F.-S. Pavone, "Optical recording of electrical activity in intact neuronal networks with random access second-harmonic generation microscopy," Opt. Express 16, 14910-14921 (2008). [PubMed]
  11. A.-C. Millard, M. Terasaki, and L.-M. Loew, "Second Harmonic Imaging of Exocytosis at Fertilization," Biophys. J. 88, L46-L48 (2005). [CrossRef] [PubMed]
  12. C. Soeller and M.-B. Cannell, "Construction of a two-photon microscope and optimisation of illumination pulse duration," Pflgers Archiv European Journal of Physiology 432, 555-561 (1996). [CrossRef]
  13. R. Hellwarth and P. Christensen "Nonlinear microscopic examination of structure in polycrystalline ZnSe," Opt. Commun. 12, 318-322 (1974). [CrossRef]
  14. R. Hellwarth and P. Christensen, "Nonlinear optical microscope using second-harmonic generation," Appl. Opt. 14, 247-248 (1975). [CrossRef] [PubMed]
  15. J. N. Gannaway and C. J. R. Sheppard, "Second harmonic imaging in the scanning optical microscope," Opt. Quantum Electron. 10, 435-439 (1978). [CrossRef]
  16. C. Sun, "Higher harmonic generation microscopy," Adv. Biochem. Eng. Biot. 95, 17-56, (2005).
  17. J. Squier and M. M¨uller, "High resolution nonlinear microscopy: A review of sources and methods for achieving optimal imaging," Rev. Sci. Instrum. 72, 2855-2867 (2001). [CrossRef]
  18. K. E. Sheetz and J. Squier, "Ultrafast optics: Imaging and manipulating biological systems," J. Appl. Phys. 105, 051101 (2009). [CrossRef]
  19. P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, "Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues," Biophys. J. 82, 493-508 (2002). [CrossRef]
  20. P. J. Campagnola and L. M. Loew, "Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms," Nat. Biotechnol. 21, 1356-1360 (2003). [CrossRef] [PubMed]
  21. W. A. Mohler, A. C. Millard, and P. J. Campagnola, "Second harmonic generation imaging of endogenous structural proteins," Methods 29, 97-109 (2003). [CrossRef] [PubMed]
  22. Y. Guo, P. P. Ho, A. Tirksliunas, F. Liu, and R. R. Alfano, "Optical harmonic generation from animal tissues by the use of picosecond and femtosecond laser pulses," Appl. Opt. 35, 6810-6813 (1996). [CrossRef] [PubMed]
  23. S. W. Chu, I. H. Chen, T. M. Liu, P. C. Chen, C. K. Sun, and B. L. Lin, "Multimodal nonlinear spectral microscopy based on a femtosecond Cr:forsterite laser," Opt. Lett. 26, 1909-1911 (2001). [CrossRef]
  24. G. Peleg, A. Lewis, M. Linial, and L. M. Loew, "Nonlinear optical measurement of membrane potential around single molecules at selected cellular sites," P. Natl. Acad. Sci. U.S.A. 96, 6700-6704 (1999). [CrossRef]
  25. P. J. Campagnola, M. D. Wei, A. Lewis, and L. M. Loew, "High-resolution nonlinear optical imaging of live cells by second harmonic generation," Biophys. J. 77, 3341-3349 (1999). [CrossRef] [PubMed]
  26. L. Moreaux, O. Sandre, and S. Charpak, "Coherent scattering in multi-harmonic light microscopy," Biophys. J. 80, 1568-1574 (2001). [CrossRef] [PubMed]
  27. Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, "Nonlinear scanning laser microscopy by third harmonic generation," Appl. Phys. Lett. 70, 922-924 (1997). [CrossRef]
  28. J. A. Squier, M. Muller, G. J. Brakenhoff, and K. R. Wilson, "Third harmonic generation microscopy," Opt. Express 3, 315-324 (1998). [CrossRef] [PubMed]
  29. M. Muller, J. Squier, K. R. Wilson, and G. J. Brakenhoff, "3D microscopy of transparent objects using thirdharmonic generation," J. Microsc.-Oxford 191, 266-274 (1998). [CrossRef]
  30. M. D. Duncan, J. Reintjes, and T. J. Manuccia, "Scanning coherent anti-Stokes Raman microscope," Opt. Lett. 7, 350-352 (1982). [CrossRef] [PubMed]
  31. A. Zumbusch, G. R. Holtom, and X. S. Xie, "Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering," Biophys. J. 82, 4142-4145 (1999).
  32. E. Cuche, P. Marquet, and C. Depeursinge, "Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms," Appl. Opt. 38, 6994-7001 (1999). [CrossRef]
  33. F. Palacios, J. Ricardo, D. Palacios, E. Goncalves, J. L. Valin, and R. De Souza, "3D image reconstruction of transparent microscopic objects using digital holography," Opt. Commun. 248, 41-50 (2005). [CrossRef]
  34. U. Schnars and W. Juptner, "Direct recording of holograms by a CCD target and numerical reconstruction," Appl. Opt. 33, 179-181 (1994). [CrossRef] [PubMed]
  35. U. Schnars and W. P. O. Juptner, "Digital recording and numerical reconstruction of holograms", Meas. Sci. Technol. 13, R85-R101 (2002). [CrossRef]
  36. J. W. Goodman, Fourier Optics (3rd ed., Roberts & Company, Greenwood Village, CO, 2005).
  37. E. N. Leith and J. Upatniek, "Reconstructed wavefronts and communication theory," J. Opt. Soc. Am. 52, 1123-1128 (1962). [CrossRef]
  38. E. N. Leith and J. Upatniek, "Microscopy by wavefront reconstruction,". J. Opt. Soc. Am. 55, 569-570 (1965). [CrossRef]
  39. Y. Pu, M. Centurion and D. Psaltis, "Harmonic holography: a new holographic principle," Appl. Opt. 47, 103-110 (2008). [CrossRef]
  40. C. L. Hsieh, R. Grange, Y. Pu, and D. Psaltis, "Three-dimensional harmonic holographic microcopy using nanoparticles as probes for cell imaging," Opt. Express 17, 2880-2891 (2009). [CrossRef] [PubMed]
  41. E. Shaffer, N. Pavillon, J. Kuhn, and C. Depeursinge, "Digital holographic microscopy investigation of second harmonic generated at a glass/air interface," Opt. Lett. 34, 2450-2452 (2009). [CrossRef] [PubMed]
  42. V. Tuchin, Tissue Optics: Light Scattering Methods and Instruments for Medical Diagnosis (2nd ed., SPIE, Belingham, WA, 2007).
  43. L. Novotny and B. Hecht, Principles of nano-optics (Cambridge University Press, Cambridge, UK, 2007).
  44. R. A. Bartels, A. Paul, H. Green, H. C. Kapteyn, M. M. Murnane, S. Backus, I. P. Christov, Y. W. Liu, D. Attwood, and C. Jacobsen, "Generation of spatially coherent light at extreme ultraviolet wavelengths," Science 297, 376-378 (2002). [PubMed]
  45. T. Colomb, J. Kuhn, F. Charriere, and C. Depeursing, "Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram," Opt. Express 14, 4300-4306 (2006). [CrossRef] [PubMed]
  46. S. D. Nocola, P. Ferraro, A Finizio, and G. Pierattini, "Wave front reconstruction of Fresnel off-axis holograms with compensation of aberrations by means of phase-shifting digital holography," Opt. Laser Eng. 37, 331-340 (2002). [CrossRef]
  47. T. Colomb, E. Cuche, F Charriere, J. Huhn, N. Aspert, F. Montfort, P. Marquet, and C. Depeursinge, "Automatic procedure for aberration compensation in digital holographic microscopy and applications to specimen shape compensation," Appl. Opt. 45, 851-863 (2006). [CrossRef] [PubMed]
  48. G. Cox, E. Kable, A. Jones, I. Fraser, F. Manconi, and M. D. Gorrell, "3-dimensional imaging of collagen using second harmonic generation," J. Struct. Biol. 141, 53-62 (2003). [CrossRef] [PubMed]
  49. S. Roth and I. Freund, "Coherent optical harmonic generation in rat-tail," Opt. Commun. 33, 292-296 (1980). [CrossRef]
  50. I. Freund, M. Deutsch, and A. Sprecher, "Optical second-harmonic microscopy, crossed-beam summation, and small-angle scattering in rat-tail tendon," Biophys. J. 50, 693-712 (1986). [CrossRef] [PubMed]
  51. P. J. Campagnola, H. A. Clark, W. A. Mohler, A. Lewis, and L. M. Loew, "Second-harmonic imaging microscopy of living cells," J. Biomed. Opt. 6, 277-286 (2001). [CrossRef] [PubMed]
  52. A. T. Yeh, N. Nassif, A. Zoumi, and B. J. Tromberg, "Selective corneal imaging using combined second-harmonic generation and two-photon excited fluorescence," Opt. Lett. 27, 2082-2084 (2002). [CrossRef]
  53. F. Tiaho, G. Recher, and D. Rouede, "Estimation of helical angles of myosin and collagen by second harmonic generation imaging microscopy," Opt. Express 15, 12286-12295 (2007). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Supplementary Material


» Media 1: MOV (3500 KB)     
» Media 2: AVI (14146 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited