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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 10 — May. 10, 2010
  • pp: 9840–9851

Label-free second harmonic generation holographic microscopy of biological specimens

Omid Masihzadeh, Philip Schlup, and Randy A. Bartels  »View Author Affiliations

Optics Express, Vol. 18, Issue 10, pp. 9840-9851 (2010)

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Second-order nonlinear holographic microscopy for highspeed, three-dimensional imaging is demonstrated. The use of harmonic generation allows image formation of endogenous features in biological samples such as muscle tissue. We have acquired holograms with acquisition times as short as 10 ms, limited by the switching speed of our shutter; frame rates of 100’s of Hz are expected to be possible. The samples are imaged with a Yb:KGW femtosecond laser oscillator, whose 1027 nm wavelength is well suited to minimize absorption and scattering. The low average power of the oscillator prevents damage to the sample.

© 2010 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(190.7110) Nonlinear optics : Ultrafast nonlinear optics
(090.1995) Holography : Digital holography

ToC Category:

Original Manuscript: April 8, 2010
Manuscript Accepted: April 23, 2010
Published: April 27, 2010

Virtual Issues
Vol. 5, Iss. 9 Virtual Journal for Biomedical Optics

Omid Masihzadeh, Philip Schlup, and Randy A. Bartels, "Label-free second harmonic generation holographic microscopy of biological specimens," Opt. Express 18, 9840-9851 (2010)

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