OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 11 — May. 24, 2010
  • pp: 11472–11482

2D label-free imaging of resonant grating biochips in ultraviolet

K. Bougot-Robin, J-L. Reverchon, M. Fromant, L. Mugherli, P. Plateau, and H. Benisty  »View Author Affiliations

Optics Express, Vol. 18, Issue 11, pp. 11472-11482 (2010)

View Full Text Article

Enhanced HTML    Acrobat PDF (1043 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



2D images of label-free biochips exploiting resonant waveguide grating (RWG) are presented. They indicate sensitivities on the order of 1 pg/mm2 for proteins in air, and hence 10 pg/mm2 in water can be safely expected. A 320×256 pixels Aluminum-Gallium-Nitride-based sensor array is used, with an intrinsic narrow spectral window centered at 280 nm. The additional role of characteristic biological layer absorption at this wavelength is calculated, and regimes revealing its impact are discussed. Experimentally, the resonance of a chip coated with protein is revealed and the sensitivity evaluated through angular spectroscopy and imaging. In addition to a sensitivity similar to surface plasmon resonance (SPR), the RWGs resonance can be flexibly tailored to gain spatial, biochemical, or spectral sensitivity.

© 2010 OSA

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(300.1030) Spectroscopy : Absorption
(280.1415) Remote sensing and sensors : Biological sensing and sensors
(310.2785) Thin films : Guided wave applications
(050.5745) Diffraction and gratings : Resonance domain

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: March 26, 2010
Revised Manuscript: May 5, 2010
Manuscript Accepted: May 6, 2010
Published: May 14, 2010

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

K. Bougot-Robin, J-L. Reverchon, M. Fromant, L. Mugherli, P. Plateau, and H. Benisty, "2D label-free imaging of resonant grating biochips in ultraviolet," Opt. Express 18, 11472-11482 (2010)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. A. Hessel and A. A. Oliner, “A New Theory of Wood’s Anomalies on Optical Gratings,” Appl. Opt. 4(10), 1275–1297 (1965). [CrossRef]
  2. U. Fano, “Effects of Configuration Interaction on Intensities and Phase Shifts,” Phys. Rev. 124(6), 1866–1878 (1961). [CrossRef]
  3. I. E. Araci, S. B. Mendes, N. Yurt, S. Honkanen, and N. Peyghambarian, “Highly sensitive spectroscopic detection of heme-protein submonolayer films by channel integrated optical waveguide,” Opt. Express 15(9), 5595–5603 (2007). [CrossRef] [PubMed]
  4. M. Nakkach, P. Lecaruyer, F. Bardin, J. Sakly, Z. Ben Lakhdar, and M. Canva, “Absorption and related optical dispersion effects on the spectral response of a surface plasmon resonance sensor,” Appl. Opt. 47(33), 6177–6182 (2008). [CrossRef] [PubMed]
  5. I. D. Block, P. C. Mathias, N. Ganesh, S. I. Jones, B. R. Dorvel, V. Chaudhery, L. O. Vodkin, R. Bashir, and B. T. Cunningham, “A detection instrument for enhanced-fluorescence and label-free imaging on photonic crystal surfaces,” Opt. Express 17(15), 13222–13235 (2009). [CrossRef] [PubMed]
  6. I. D. Block, P. C. Mathias, S. I. Jones, L. O. Vodkin, and B. T. Cunningham, “Optimizing the spatial resolution of photonic crystal label-free imaging,” Appl. Opt. 48(34), 6567–6574 (2009). [CrossRef] [PubMed]
  7. R. B. M. Schasfoort, and A. McWhirter, in Handbook of surface plasmon resonance (R.B.M Schasfoort and A. J. Tudos, RSC Publishing Enschede, Netherlands, 2007), Chap. 3.
  8. P. Y. Li, B. Lin, J. Gerstenmaier, and B. T. Cunningham, “A new method for label-free imaging of biomolecular interactions,” Sens. Act. Chem. 99(1), 6–13 (2004). [CrossRef]
  9. E. M. Yeatman, “Resolution and sensitivity in surface plasmon microscopy and sensing,” Bios. Bioelec. 11(6-7), 635–649 (1996). [CrossRef]
  10. N. Ganesh, I. D. Block, and B. T. Cunningham, “Near ultraviolet-wavelength photonic-crystal biosensor with enhanced surface-to-bulk sensitivity ratio,” Appl. Phys. Lett. 89(2), 023901 (2006). [CrossRef]
  11. K. Robin, J. L. Reverchon, L. Mugherli, M. Fromant, P. Plateau, and H. Benisty, “Detection of biological macromolecules on a biochip dedicated to UV specific absorption,” Bios. Bioelec. 24(6), 1585–1591 (2009). [CrossRef]
  12. J. L. Reverchon, J. A. Robo, J. P. Truffer, J. P. Caumes, I. Mourad, J. Brault, and J. Y. Duboz, “AlGaN-based focal plane arrays for selective UV imaging at 310nm and 280nm and route toward deep UV imaging”, Proc. SPIE 6744 (2007). [CrossRef]
  13. K. Robin, J. L. Reverchon, L. Mugherli, M. Fromant, and H. Benisty, “Biodetection of DNA and proteins using enhanced UV absorption by structuration of the chip surface”, Proc. SPIE 7188-04 (2009). [CrossRef]
  14. L. Li, “Formulation and comparison of two recursive matrix algorithms for modeling layered diffraction gratings,” J. Opt. Soc. Am. A 13(5), 1024–1035 (1996). [CrossRef]
  15. A. David, “High efficiency GaN-based LEDs: light extraction by photonic crystals,” Ann. Phys. (Paris) 31(6), 1–235 (2006).
  16. F. David, Edwards, “Silicon (Si)” in Handbook of optical constants of Solids, E. D. Palik ed.(Academic, Press, 1985).
  17. V. Brioude and O. Parriaux, “Normalised analysis for the design of evanescent-wave sensors and its use for tolerance evaluation,” Opt. Quant. Elec. 32(6/8), 899–908 (2000). [CrossRef]
  18. D. Bahatt, J. E. Cahill, K. Nishikida, E. G. Picozza, P. G. Saviano, D. H. Tracy, and Y. Wang, “Optical resonance analysis system”, US patent 7251085, (2007).
  19. E. Özkumur, J. W. Needham, D. A. Bergstein, R. Gonzalez, M. Cabodi, J. M. Gershoni, B. B. Goldberg, and M. S. Unlü, “Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications,” Proc. Natl. Acad. Sci. U.S.A. 105(23), 7988–7992 (2008). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited