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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 11 — May. 24, 2010
  • pp: 11472–11482

2D label-free imaging of resonant grating biochips in ultraviolet

K. Bougot-Robin, J-L. Reverchon, M. Fromant, L. Mugherli, P. Plateau, and H. Benisty  »View Author Affiliations


Optics Express, Vol. 18, Issue 11, pp. 11472-11482 (2010)
http://dx.doi.org/10.1364/OE.18.011472


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Abstract

2D images of label-free biochips exploiting resonant waveguide grating (RWG) are presented. They indicate sensitivities on the order of 1 pg/mm2 for proteins in air, and hence 10 pg/mm2 in water can be safely expected. A 320×256 pixels Aluminum-Gallium-Nitride-based sensor array is used, with an intrinsic narrow spectral window centered at 280 nm. The additional role of characteristic biological layer absorption at this wavelength is calculated, and regimes revealing its impact are discussed. Experimentally, the resonance of a chip coated with protein is revealed and the sensitivity evaluated through angular spectroscopy and imaging. In addition to a sensitivity similar to surface plasmon resonance (SPR), the RWGs resonance can be flexibly tailored to gain spatial, biochemical, or spectral sensitivity.

© 2010 OSA

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(300.1030) Spectroscopy : Absorption
(280.1415) Remote sensing and sensors : Biological sensing and sensors
(310.2785) Thin films : Guided wave applications
(050.5745) Diffraction and gratings : Resonance domain

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: March 26, 2010
Revised Manuscript: May 5, 2010
Manuscript Accepted: May 6, 2010
Published: May 14, 2010

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

Citation
K. Bougot-Robin, J-L. Reverchon, M. Fromant, L. Mugherli, P. Plateau, and H. Benisty, "2D label-free imaging of resonant grating biochips in ultraviolet," Opt. Express 18, 11472-11482 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-11-11472


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