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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 12 — Jun. 7, 2010
  • pp: 13136–13150

Zeeman laser scanning confocal microscope and its ability on reduction of specimen-induced spherical aberration

Jheng-Syong Wu, Chien Chou, Chi-Hui Chang, Li-Ping Yu, Li-Dek Chou, Hsiu-Fong Chang, Hon-Fai Yau, and Cheng-Chung Lee  »View Author Affiliations


Optics Express, Vol. 18, Issue 12, pp. 13136-13150 (2010)
http://dx.doi.org/10.1364/OE.18.013136


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Abstract

The spherical aberration induced by refractive-index mismatch results in the degradation on the quality of sectioning images in conventional confocal laser scanning microscope (CLSM). In this research, we have derived the theory of image formation in a Zeeman laser scanning confocal microscope (ZLSCM) and conducted experiments in order to verify the ability of reducing spherical aberration in ZLSCM. A Zeeman laser is used as the light source and produces the linearly polarized photon-pairs (LPPP) laser beam. With the features of common-path propagation of LPPP and optical heterodyne detection, ZLSCM shows the ability of reducing the specimen-induced spherical aberration and improving the axial resolution simultaneously.

© 2010 OSA

OCIS Codes
(080.1010) Geometric optics : Aberrations (global)
(110.2990) Imaging systems : Image formation theory
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(180.1790) Microscopy : Confocal microscopy

ToC Category:
Microscopy

History
Original Manuscript: March 23, 2010
Revised Manuscript: May 14, 2010
Manuscript Accepted: May 14, 2010
Published: June 3, 2010

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Jheng-Syong Wu, Chien Chou, Chi-Hui Chang, Li-Ping Yu, Li-Dek Chou, Hsiu-Fong Chang, Hon-Fai Yau, and Cheng-Chung Lee, "Zeeman laser scanning confocal microscope and its ability on reduction of specimen-induced spherical aberration," Opt. Express 18, 13136-13150 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-12-13136


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