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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 13 — Jun. 21, 2010
  • pp: 13516–13528

Applicability of an EM-CCD for spatially resolved TIR-ICS

Daniel Boening, Teja W. Groemer, and Jurgen Klingauf  »View Author Affiliations

Optics Express, Vol. 18, Issue 13, pp. 13516-13528 (2010)

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In this work we systematically explored performance of an EM-CCD as a detector for spatially resolved total internal reflection image correlation spectroscopy (TIR-ICS) with respect to adjustable parameters. We show that variations in the observation volume (pixel binning) can be well described by a simple structural term ω. To test the sensitivity of camera-based TIR-ICS we measured diffusion coefficients and particle numbers (PN) of fluorescent probes of different sizes (Fluorospheres, GFP and labeled antibodies) at varying viscosities, concentrations, and sampling rates. TIR-ICS allowed distinguishing between different probe concentrations with differences in PN of 5% and differences of 6% in D by acquiring only 15 independent measurement runs.

© 2010 OSA

OCIS Codes
(040.1520) Detectors : CCD, charge-coupled device
(100.4550) Image processing : Correlators
(180.0180) Microscopy : Microscopy
(260.2510) Physical optics : Fluorescence
(260.6970) Physical optics : Total internal reflection
(300.0300) Spectroscopy : Spectroscopy

ToC Category:

Original Manuscript: April 12, 2010
Revised Manuscript: May 27, 2010
Manuscript Accepted: May 27, 2010
Published: June 8, 2010

Virtual Issues
Vol. 5, Iss. 11 Virtual Journal for Biomedical Optics

Daniel Boening, Teja W. Groemer, and Jurgen Klingauf, "Applicability of an EM-CCD for spatially resolved TIR-ICS," Opt. Express 18, 13516-13528 (2010)

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