A compact acousto-optic lens for 2D and 3D femtosecond based 2-photon microscopy

doi:10.1364/OE.18.013720

A compact acousto-optic lens for 2D and 3D femtosecond based 2-photon microscopy

Paul A. Kirkby, K. M. Naga Srinivas Nadella, and R. Angus Silver »View Author Affiliations

Optics Express, Vol. 18, Issue 13, pp. 13720-13744 (2010)
http://dx.doi.org/10.1364/OE.18.013720

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Abstract

We describe a high speed 3D Acousto-Optic Lens Microscope (AOLM) for femtosecond 2-photon imaging. By optimizing the design of the 4 AO Deflectors (AODs) and by deriving new control algorithms, we have developed a compact spherical AOL with a low temporal dispersion that enables 2-photon imaging at 10-fold lower power than previously reported. We show that the AOLM can perform high speed 2D raster-scan imaging (>150 Hz) without scan rate dependent astigmatism. It can deflect and focus a laser beam in a 3D random access sequence at 30 kHz and has an extended focusing range (>137 μm; 40X 0.8NA objective). These features are likely to make the AOLM a useful tool for studying fast physiological processes distributed in 3D space

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(230.1040) Optical devices : Acousto-optical devices
(320.2250) Ultrafast optics : Femtosecond phenomena

ToC Category:
Microscopy

History
Original Manuscript: March 15, 2010
Revised Manuscript: June 1, 2010
Manuscript Accepted: June 6, 2010
Published: June 11, 2010

Virtual Issues
Vol. 5, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Paul A. Kirkby, K. M. Naga Srinivas Nadella, and R. Angus Silver, "A compact acousto-optic lens for 2D and 3D femtosecond based 2-photon microscopy," Opt. Express 18, 13720-13744 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-13-13720

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References

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003). [CrossRef] [PubMed]
K. Svoboda and R. Yasuda, “Principles of two-photon excitation microscopy and its applications to neuroscience,” Neuron 50(6), 823–839 (2006). [CrossRef] [PubMed]
W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990). [CrossRef] [PubMed]
F. Helmchen and W. Denk, “Deep tissue two-photon microscopy,” Nat. Methods 2(12), 932–940 (2005). [CrossRef] [PubMed]
J. B. Pawley, Handbook of Biological Confocal Microscopy (Plenum Press, New York, 1995).
J. D. Lechleiter, D. T. Lin, and I. Sieneart, “Multi-photon laser scanning microscopy using an acoustic optical deflector,” Biophys. J. 83(4), 2292–2299 (2002). [CrossRef] [PubMed]
R. D. Roorda, T. M. Hohl, R. Toledo-Crow, and G. Miesenböck, “Video-rate nonlinear microscopy of neuronal membrane dynamics with genetically encoded probes,” J. Neurophysiol. 92(1), 609–621 (2004). [CrossRef] [PubMed]
V. Iyer, B. E. Losavio, and P. Saggau, “Compensation of spatial and temporal dispersion for acousto-optic multiphoton laser-scanning microscopy,” J. Biomed. Opt. 8(3), 460–471 (2003). [CrossRef] [PubMed]
X. Lv, C. Zhan, S. Zeng, W. R. Chen, and Q. Luo, “Construction of multiphoton laser scanning microscope based on dual-axis acousto-optic deflector,” Rev. Sci. Instrum. 77(4), 046101–046103 (2006). [CrossRef]
V. Iyer, T. M. Hoogland, and P. Saggau, “Fast functional imaging of single neurons using random-access multiphoton (RAMP) microscopy,” J. Neurophysiol. 95(1), 535–545 (2005). [CrossRef] [PubMed]
R. Salomé, Y. Kremer, S. Dieudonné, J. F. Léger, O. Krichevsky, C. Wyart, D. Chatenay, and L. Bourdieu, “Ultrafast random-access scanning in two-photon microscopy using acousto-optic deflectors,” J. Neurosci. Methods 154(1-2), 161–174 (2006). [CrossRef] [PubMed]
Y. Otsu, V. Bormuth, J. Wong, B. Mathieu, G. P. Dugué, A. Feltz, and S. Dieudonné, “Optical monitoring of neuronal activity at high frame rate with a digital random-access multiphoton (RAMP) microscope,” J. Neurosci. Methods 173(2), 259–270 (2008). [CrossRef] [PubMed]
Y. Kremer, J. F. Léger, R. Lapole, N. Honnorat, Y. Candela, S. Dieudonné, and L. Bourdieu, “A spatio-temporally compensated acousto-optic scanner for two-photon microscopy providing large field of view,” Opt. Express 16(14), 10066–10076 (2008). [CrossRef] [PubMed]
S. Shoham, D. H. O’Connor, D. V. Sarkisov, and S. S. Wang, “Rapid neurotransmitter uncaging in spatially defined patterns,” Nat. Methods 2(11), 837–843 (2005). [CrossRef] [PubMed]
B. Losavio, V. Iyer, and P. Saggau, “Two photon microscope for multisite microphotolysis of caged neurotransmitters in acute brain slices,” J. Biomed. Opt . 14, 064033 064031–064013 (2009). [CrossRef]
J. Xu, and R. Stroud, Acousto-Optic Devices, Principles, Design and Applications (John Wiley and Sons Inc., 1992).
N. Friedman, A. Kaplan, and N. Davidson, “Acousto-optic scanning system with very fast nonlinear scans,” Opt. Lett. 25(24), 1762–1764 (2000). [CrossRef]
W. Göbel, B. M. Kampa, and F. Helmchen, “Imaging cellular network dynamics in three dimensions using fast 3D laser scanning,” Nat. Methods 4(1), 73–79 (2007). [CrossRef]
E. J. Botcherby, R. Juskaitis, M. J. Booth, and T. Wilson, “Aberration-free optical refocusing in high numerical aperture microscopy,” Opt. Lett. 32(14), 2007–2009 (2007). [CrossRef] [PubMed]
A. Kaplan, N. Friedman, and N. Davidson, “Acousto-optic lens with very fast focus scanning,” Opt. Lett. 26(14), 1078–1080 (2001). [CrossRef]
G. Duemani Reddy, K. Kelleher, R. Fink, and P. Saggau, “Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity,” Nat. Neurosci. 11(6), 713–720 (2008). [CrossRef] [PubMed]
G. D. Reddy and P. Saggau, “Fast three-dimensional laser scanning scheme using acousto-optic deflectors,” J. Biomed. Opt. 10(6), 064038 (2005). [CrossRef]
G. Reddy, “A multiphoton microscope for three dimensional functional recording of fast neuronal activity,” (Rice University, Houston Texas USA, 2007).
P. A. Kirkby, R. A. Silver, and K. M. N. S. Nadella, “IMAGING APPARATUS AND METHODS,” (20.03.2008).
D. Vucinić, T. J. Sejnowski, and B. Lu, “A compact multiphoton 3D imaging system for recording fast neuronal activity,” PLoS ONE 2(8), e699 (2007). [CrossRef] [PubMed]
Z. Kam, D. A. Agard, and J. W. Sedat, “Three-dimensional microscopy in thick biological samples: A fresh approach for adjusting focus and correcting spherical aberration,” Bioimaging 5(1), 40–49 (1997). [CrossRef]
A. P. Goutzoulis, D. R. Pape, and S. V. Kulakov, Design and Fabrication of Acousto Optic Devices (Marcel Dekker, 1994).
E. H. Young, H. C. Ho, and L. J. Harrison, “Optically rotated long time aperture TeO2 Bragg cell,” Proc. SPIE 1296, 304–315 (1990). [CrossRef]
B. K. A. Ngoi, K. Venkatakrishnan, L. E. Lim, B. Tan, and L. E. N. Lim, “Angular dispersion compensation for acousto-optic devices used for ultrashort-pulsed laser micromachining,” Opt. Express 9(4), 200–206 (2001). [CrossRef] [PubMed]
D. Reddy, and P. Saggau, “Fast Three-Dimensional Random Access Multi-Photon Microscopy for Functional Recording of Neuronal Activity,” Proc. SPIE 6630, 66301A 66301–66308 (2007).
L. Zhu, P. C. Sun, and Y. Fainman, “Aberration-free dynamic focusing with a multichannel micromachined membrane deformable mirror,” Appl. Opt. 38(25), 5350–5354 (1999). [CrossRef]

Appl. Opt.

L. Zhu, P. C. Sun, and Y. Fainman, “Aberration-free dynamic focusing with a multichannel micromachined membrane deformable mirror,” Appl. Opt. 38(25), 5350–5354 (1999). [CrossRef]

Bioimaging

Z. Kam, D. A. Agard, and J. W. Sedat, “Three-dimensional microscopy in thick biological samples: A fresh approach for adjusting focus and correcting spherical aberration,” Bioimaging 5(1), 40–49 (1997). [CrossRef]

Biophys. J.

J. D. Lechleiter, D. T. Lin, and I. Sieneart, “Multi-photon laser scanning microscopy using an acoustic optical deflector,” Biophys. J. 83(4), 2292–2299 (2002). [CrossRef] [PubMed]

J. Biomed. Opt

B. Losavio, V. Iyer, and P. Saggau, “Two photon microscope for multisite microphotolysis of caged neurotransmitters in acute brain slices,” J. Biomed. Opt . 14, 064033 064031–064013 (2009). [CrossRef]

J. Biomed. Opt.

V. Iyer, B. E. Losavio, and P. Saggau, “Compensation of spatial and temporal dispersion for acousto-optic multiphoton laser-scanning microscopy,” J. Biomed. Opt. 8(3), 460–471 (2003). [CrossRef] [PubMed]
G. D. Reddy and P. Saggau, “Fast three-dimensional laser scanning scheme using acousto-optic deflectors,” J. Biomed. Opt. 10(6), 064038 (2005). [CrossRef]

J. Neurophysiol.

R. D. Roorda, T. M. Hohl, R. Toledo-Crow, and G. Miesenböck, “Video-rate nonlinear microscopy of neuronal membrane dynamics with genetically encoded probes,” J. Neurophysiol. 92(1), 609–621 (2004). [CrossRef] [PubMed]
V. Iyer, T. M. Hoogland, and P. Saggau, “Fast functional imaging of single neurons using random-access multiphoton (RAMP) microscopy,” J. Neurophysiol. 95(1), 535–545 (2005). [CrossRef] [PubMed]

J. Neurosci. Methods

R. Salomé, Y. Kremer, S. Dieudonné, J. F. Léger, O. Krichevsky, C. Wyart, D. Chatenay, and L. Bourdieu, “Ultrafast random-access scanning in two-photon microscopy using acousto-optic deflectors,” J. Neurosci. Methods 154(1-2), 161–174 (2006). [CrossRef] [PubMed]
Y. Otsu, V. Bormuth, J. Wong, B. Mathieu, G. P. Dugué, A. Feltz, and S. Dieudonné, “Optical monitoring of neuronal activity at high frame rate with a digital random-access multiphoton (RAMP) microscope,” J. Neurosci. Methods 173(2), 259–270 (2008). [CrossRef] [PubMed]

Nat. Biotechnol.

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003). [CrossRef] [PubMed]

Nat. Methods

F. Helmchen and W. Denk, “Deep tissue two-photon microscopy,” Nat. Methods 2(12), 932–940 (2005). [CrossRef] [PubMed]
S. Shoham, D. H. O’Connor, D. V. Sarkisov, and S. S. Wang, “Rapid neurotransmitter uncaging in spatially defined patterns,” Nat. Methods 2(11), 837–843 (2005). [CrossRef] [PubMed]
W. Göbel, B. M. Kampa, and F. Helmchen, “Imaging cellular network dynamics in three dimensions using fast 3D laser scanning,” Nat. Methods 4(1), 73–79 (2007). [CrossRef]

Nat. Neurosci.

G. Duemani Reddy, K. Kelleher, R. Fink, and P. Saggau, “Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity,” Nat. Neurosci. 11(6), 713–720 (2008). [CrossRef] [PubMed]

Neuron

K. Svoboda and R. Yasuda, “Principles of two-photon excitation microscopy and its applications to neuroscience,” Neuron 50(6), 823–839 (2006). [CrossRef] [PubMed]

Opt. Express

Opt. Lett.

PLoS ONE

D. Vucinić, T. J. Sejnowski, and B. Lu, “A compact multiphoton 3D imaging system for recording fast neuronal activity,” PLoS ONE 2(8), e699 (2007). [CrossRef] [PubMed]

Proc. SPIE

E. H. Young, H. C. Ho, and L. J. Harrison, “Optically rotated long time aperture TeO2 Bragg cell,” Proc. SPIE 1296, 304–315 (1990). [CrossRef]

Rev. Sci. Instrum.

X. Lv, C. Zhan, S. Zeng, W. R. Chen, and Q. Luo, “Construction of multiphoton laser scanning microscope based on dual-axis acousto-optic deflector,” Rev. Sci. Instrum. 77(4), 046101–046103 (2006). [CrossRef]

Science

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990). [CrossRef] [PubMed]

Other

J. B. Pawley, Handbook of Biological Confocal Microscopy (Plenum Press, New York, 1995).
J. Xu, and R. Stroud, Acousto-Optic Devices, Principles, Design and Applications (John Wiley and Sons Inc., 1992).
D. Reddy, and P. Saggau, “Fast Three-Dimensional Random Access Multi-Photon Microscopy for Functional Recording of Neuronal Activity,” Proc. SPIE 6630, 66301A 66301–66308 (2007).
A. P. Goutzoulis, D. R. Pape, and S. V. Kulakov, Design and Fabrication of Acousto Optic Devices (Marcel Dekker, 1994).
G. Reddy, “A multiphoton microscope for three dimensional functional recording of fast neuronal activity,” (Rice University, Houston Texas USA, 2007).
P. A. Kirkby, R. A. Silver, and K. M. N. S. Nadella, “IMAGING APPARATUS AND METHODS,” (20.03.2008).

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