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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 13 — Jun. 21, 2010
  • pp: 14063–14078

Quantitative SLM-based differential interference contrast imaging

Timothy J. McIntyre, Christian Maurer, Stephanie Fassl, Saranjam Khan, Stefan Bernet, and Monika Ritsch-Marte  »View Author Affiliations

Optics Express, Vol. 18, Issue 13, pp. 14063-14078 (2010)

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We describe the implementation of quantitative Differential Interference Contrast (DIC) Microscopy using a spatial light modulator (SLM) as a flexible Fourier filter in the optical path. The experimental arrangement allows for the all-electronic acquisition of multiple phase shifted DIC-images at video rates which are analyzed to yield the optical path length variation of the sample. The resolution of the technique is analyzed by retrieving the phase profiles of polystyrene spheres in immersion oil, and the method is then applied for quantitative imaging of biological samples. By reprogramming the diffractive structure displayed at the SLM it is possible to record the whole set of phase shifted DIC images simultaneously in different areas of the same camera chip. This allows for quantitative snap-shot imaging of a sample, which has applications for the investigation of dynamic processes.

© 2010 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.3170) Microscopy : Interference microscopy

ToC Category:

Original Manuscript: April 16, 2010
Revised Manuscript: May 28, 2010
Manuscript Accepted: June 6, 2010
Published: June 15, 2010

Virtual Issues
Vol. 5, Iss. 11 Virtual Journal for Biomedical Optics

Timothy J. McIntyre, Christian Maurer, Stephanie Fassl, Saranjam Khan, Stefan Bernet, and Monika Ritsch-Marte, "Quantitative SLM-based differential interference contrast imaging," Opt. Express 18, 14063-14078 (2010)

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