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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 14 — Jul. 5, 2010
  • pp: 14859–14870

Influence of birefringence on polarization resolved nonlinear microscopy and collagen SHG structural imaging

D. Aït-Belkacem, A. Gasecka, F. Munhoz, S. Brustlein, and S. Brasselet  »View Author Affiliations

Optics Express, Vol. 18, Issue 14, pp. 14859-14870 (2010)

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We analyze the influence of the anisotropy of molecular and biological samples on polarization resolved nonlinear microscopy imaging. We show in particular the detrimental influence of birefringence on Second Harmonic Generation (SHG) and Two-Photon Excited Fluorescence (TPEF) polarization resolved microscopy imaging, which, if not accounted for, can lead to an erroneous determination of the sample properties and thus to a misinterpretation of the read-out information. We propose a method to measure this birefringence and account for this effect in nonlinear polarization resolved experiments.

© 2010 Optical Society of America

OCIS Codes
(190.1900) Nonlinear optics : Diagnostic applications of nonlinear optics
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: May 3, 2010
Manuscript Accepted: May 26, 2010
Published: June 28, 2010

Virtual Issues
Vol. 5, Iss. 11 Virtual Journal for Biomedical Optics

Sophie Brasselet, Dora Aït-Belkacem, Alicja Gasecka, Fabiana Munhoz, Sophie Brustlein, and S. Brasselet, "Influence of birefringence on polarization resolved nonlinear microscopy and collagen SHG structural imaging," Opt. Express 18, 14859-14870 (2010)

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