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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 16 — Aug. 2, 2010
  • pp: 16289–16301

Time-resolved diffusing wave spectroscopy with a CCD camera

Katarzyna Zarychta, Eric Tinet, Leila Azizi, Sigrid Avrillier, Dominique Ettori, and Jean-Michel Tualle  »View Author Affiliations

Optics Express, Vol. 18, Issue 16, pp. 16289-16301 (2010)

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We show how time-resolved measurements of the diffuse light transmitted through a thick scattering slab can be performed with a standard CCD camera, thanks to an interferometric protocol. Time-resolved correlations measured at a fixed photon transit time are also presented. The high number of pixels of the camera allows us to attain a quite good sensitivity for a reasonably low acquisition time.

© 2010 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(110.0113) Imaging systems : Imaging through turbid media

ToC Category:
Imaging Systems

Original Manuscript: April 2, 2010
Revised Manuscript: May 31, 2010
Manuscript Accepted: June 16, 2010
Published: July 19, 2010

Virtual Issues
Vol. 5, Iss. 12 Virtual Journal for Biomedical Optics

Katarzyna Zarychta, Eric Tinet, Leila Azizi, Sigrid Avrillier, Dominique Ettori, and Jean-Michel Tualle, "Time-resolved diffusing wave spectroscopy with a CCD camera," Opt. Express 18, 16289-16301 (2010)

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  1. A. Torricelli, A. Pifferi, L. Spinelli, R. Cubeddu, F. Martelli, S. Del Bianco, and G. Zaccanti, “Time-resolved reflectance at null source-detector separation: improving contrast and resolution in diffuse optical imaging,” Phys. Rev. Lett. 95(7), 078101 (2005). [CrossRef] [PubMed]
  2. A. Pifferi, A. Torricelli, L. Spinelli, D. Contini, R. Cubeddu, F. Martelli, G. Zaccanti, A. Tosi, A. Dalla Mora, F. Zappa, and S. Cova, “Time-resolved diffuse reflectance using small source-detector separation and fast single-photon gating,” Phys. Rev. Lett. 100(13), 138101 (2008). [CrossRef] [PubMed]
  3. R. Esposito, S. De Nicola, M. Brambilla, A. Pifferi, L. Spinelli, and M. Lepore, “Depth dependence of estimated optical properties of a scattering inclusion by time-resolved contrast functions,” Opt. Express 16(22), 17667–17681 (2008), http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-22-17667 . [CrossRef] [PubMed]
  4. A. Liebert, H. Wabnitz, J. Steinbrink, H. Obrig, M. Möller, R. Macdonald, A. Villringer, and H. Rinneberg, “Time-resolved multidistance near-infrared spectroscopy of the adult head: intracerebral and extracerebral absorption changes from moments of distribution of times of flight of photons,” Appl. Opt. 43(15), 3037–3047 (2004). [CrossRef] [PubMed]
  5. A. Liebert, H. Wabnitz, N. Zołek, and R. Macdonald, “Monte Carlo algorithm for efficient simulation of time-resolved fluorescence in layered turbid media,” Opt. Express 16(17), 13188–13202 (2008), http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-17-13188 . [CrossRef] [PubMed]
  6. J. Selb, J. J. Stott, M. A. Franceschini, A. G. Sorensen, and D. A. Boas, “Improved sensitivity to cerebral hemodynamics during brain activation with a time-gated optical system: analytical model and experimental validation,” J. Biomed. Opt. 10(1), 011013 (2005). [CrossRef]
  7. A. Kienle, M. S. Patterson, N. Dögnitz, R. Bays, G. Wagniνres, and H. van den Bergh, “Noninvasive Determination of the Optical Properties of Two-Layered Turbid Media,” Appl. Opt. 37(4), 779–791 (1998). [CrossRef]
  8. L. Gagnon, C. Gauthier, R. D. Hoge, F. Lesage, J. Selb, and D. A. Boas, “Double-layer estimation of intra- and extracerebral hemoglobin concentration with a time-resolved system,” J. Biomed. Opt. 13(5), 054019 (2008). [CrossRef] [PubMed]
  9. B. Montcel, R. Chabrier, and P. Poulet, “Detection of cortical activation with time-resolved diffuse optical methods,” Appl. Opt. 44(10), 1942–1947 (2005). [CrossRef] [PubMed]
  10. J.-M. Tualle, H. L. Nghiem, D. Ettori, R. Sablong, É. Tinet, and S. Avrillier, “Asymptotic behavior and inverse problem in layered scattering media,” J. Opt. Soc. Am. A 21(1), 24–34 (2004). [CrossRef]
  11. S. Andersson-Engels, R. Berg, S. Svanberg, and O. Jarlman, “Time-resolved transillumination for medical diagnostics,” Opt. Lett. 15(21), 1179–1181 (1990). [CrossRef] [PubMed]
  12. G. Le Tolguenec, F. Devaux, and E. Lantz, “Imaging through thick biological tissues by parametric image amplification and phase conjugation,” J. Opt. 28(5), 214–217 (1997). [CrossRef]
  13. V. Chernomordik, A. Gandjbakhche, M. Lepore, R. Esposito, and I. Delfino, “Depth dependence of the analytical expression for the width of the point spread function (spatial resolution) in time-resolved transillumination,” J. Biomed. Opt. 6(4), 441–445 (2001). [CrossRef] [PubMed]
  14. L. Azizi, K. Zarychta, D. Ettori, E. Tinet, and J.-M. Tualle, “Ultimate spatial resolution with Diffuse Optical Tomography,” Opt. Express 17(14), 12132–12144 (2009), http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-14-12132 . [CrossRef] [PubMed]
  15. A. Kienle and T. Glanzmann, “In vivo determination of the optical properties of muscle with time-resolved reflectance using a layered model,” Phys. Med. Biol. 44(11), 2689–2702 (1999). [CrossRef] [PubMed]
  16. J. C. Hebden, A. Gibson, R. M. Yusof, N. Everdell, E. M. C. Hillman, D. T. Delpy, S. R. Arridge, T. Austin, J. H. Meek, and J. S. Wyatt, “Three-dimensional optical tomography of the premature infant brain,” Phys. Med. Biol. 47(23), 4155–4166 (2002). [CrossRef] [PubMed]
  17. E. M. C. Hillman, J. C. Hebden, M. Schweiger, H. Dehghani, F. E. W. Schmidt, D. T. Delpy, and S. R. Arridge, “Time resolved optical tomography of the human forearm,” Phys. Med. Biol. 46(4), 1117–1130 (2001). [CrossRef] [PubMed]
  18. T. D. Yates, J. C. Hebden, A. P. Gibson, N. L. Everdell, S. R. Arridge, and M. Douek, “Optical tomography of the breast using a multi-channel time-resolved imager,” Phys. Med. Biol. 50(11), 2503–2517 (2005). [CrossRef] [PubMed]
  19. C. V. Zint, W. Uhring, M. Torregrossa, B. Cunin, and P. Poulet, “Streak camera: a multidetector for diffuse optical tomography,” Appl. Opt. 42(16), 3313–3320 (2003). [CrossRef] [PubMed]
  20. J.-M. Tualle, B. Gélébart, E. Tinet, S. Avrillier, and J. P. Ollivier, “Real time optical coefficients evaluation from time and space resolved reflectance measurements in biological tissues,” Opt. Commun. 124(3–4), 216–221 (1996). [CrossRef]
  21. A. Pifferi, J. Swartling, E. Chikoidze, A. Torricelli, P. Taroni, A. Bassi, S. Andersson-Engels, and R. Cubeddu, “Spectroscopic time-resolved diffuse reflectance and transmittance measurements of the female breast at different interfiber distances,” J. Biomed. Opt. 9(6), 1143–1151 (2004). [CrossRef] [PubMed]
  22. D. Grosenick, K. T. Moesta, M. Möller, J. Mucke, H. Wabnitz, B. Gebauer, C. Stroszczynski, B. Wassermann, P. M. Schlag, and H. Rinneberg, “Time-domain scanning optical mammography: I. Recording and assessment of mammograms of 154 patients,” Phys. Med. Biol. 50(11), 2429–2449 (2005). [CrossRef] [PubMed]
  23. P. Taroni, A. Torricelli, L. Spinelli, A. Pifferi, F. Arpaia, G. Danesini, and R. Cubeddu, “Time-resolved optical mammography between 637 and 985 nm: clinical study on the detection and identification of breast lesions,” Phys. Med. Biol. 50(11), 2469–2488 (2005). [CrossRef] [PubMed]
  24. R. Cubeddu, A. Pifferi, P. Taroni, A. Torricelli, and G. Valentini, “Compact tissue oximeter based on dual-wavelength multichannel time-resolved reflectance,” Appl. Opt. 38(16), 3670–3680 (1999). [CrossRef]
  25. J.-M. Tualle, “Method for analyzing a diffusing sample by time resolution measurement”, US Patent 6903825 (2001).
  26. J.-M. Tualle, E. Tinet, and S. Avrillier, “A new and easy way to perform time-resolved measurements of the light scattered by a turbid medium,” Opt. Commun. 189(4–6), 211–220 (2001). [CrossRef]
  27. J.-M. Tualle, H. L. Nghiêm, C. Schäfauer, P. Berthaud, É. Tinet, D. Ettori, and S. Avrillier, “Time-resolved measurements from speckle interferometry,” Opt. Lett. 30(1), 50–52 (2005). [CrossRef] [PubMed]
  28. J.-M. Tualle, H. L. Nghiêm, M. Cheikh, D. Ettori, E. Tinet, and S. Avrillier, “Time-resolved diffusing wave spectroscopy beyond 300 transport mean free paths,” J. Opt. Soc. Am. A 23(6), 1452 (2006). [CrossRef]
  29. M. Cheikh, H. L. Nghiêm, D. Ettori, E. Tinet, S. Avrillier, and J. M. Tualle, “Time-resolved diffusing wave spectroscopy applied to dynamic heterogeneity imaging,” Opt. Lett. 31(15), 2311–2313 (2006). [CrossRef] [PubMed]
  30. G. Maret and P. E. Wolf, “Multiple light scattering from disordered media. The effect of Brownian motion of scatterers,” Z. Phys. B Condens. Matter 65(4), 409–413 (1987). [CrossRef]
  31. M. J. Stephen, “Temporal fluctuations in wave propagation in random media,” Phys. Rev. B Condens. Matter 37(1), 1–5 (1988). [CrossRef] [PubMed]
  32. D. J. Pine, D. A. Weitz, P. M. Chaikin, and E. Herbolzheimer, “Diffusing wave spectroscopy,” Phys. Rev. Lett. 60(12), 1134–1137 (1988). [CrossRef] [PubMed]
  33. X. L. Wu, D. J. Pine, P. M. Chaikin, J. S. Huang, and D. A. Weitz, “Diffusing-wave spectroscopy in a shear flow,” J. Opt. Soc. Am. B 7(1), 15–20 (1990). [CrossRef]
  34. T. Durduran, R. Choe, G. Yu, C. Zhou, J. C. Tchou, B. J. Czerniecki, and A. G. Yodh, “Diffuse optical measurement of blood flow in breast tumors,” Opt. Lett. 30(21), 2915–2917 (2005). [CrossRef] [PubMed]
  35. J. Li, F. Jaillon, G. Dietsche, G. Maret, and T. Gisler, “Pulsation-resolved deep tissue dynamics measured with diffusing-wave spectroscopy,” Opt. Express 14(17), 7841–7851 (2006), http://www.opticsinfobase.org/abstract.cfm?URI=oe-14-17-7841 . [CrossRef] [PubMed]
  36. L. Gagnon, M. Desjardins, J. Jehanne-Lacasse, L. Bherer, and F. Lesage, “Investigation of diffuse correlation spectroscopy in multi-layered media including the human head,” Opt. Express 16(20), 15514–15530 (2008), http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-20-15514 . [CrossRef] [PubMed]
  37. G. Dietsche, M. Ninck, C. Ortolf, J. Li, F. Jaillon, and T. Gisler, “Fiber-based multispeckle detection for time-resolved diffusing-wave spectroscopy: characterization and application to blood flow detection in deep tissue,” Appl. Opt. 46(35), 8506–8514 (2007). [CrossRef] [PubMed]
  38. T. Spirig, P. Seitz, O. Vietze, and F. Heitger, “The lock-in CCD- two-dimensional synchronous detection of light,” IEEE J. Quantum Electron. 31(9), 1705–1708 (1995). [CrossRef]
  39. P. Gleyzes, A. C. Boccara, and H. Saint-Jalmes, “Multichannel Nomarski microscope with polarization modulation: performance and applications,” Opt. Lett. 22(20), 1529–1531 (1997). [CrossRef]
  40. M. Gross, P. Goy, B. C. Forget, M. Atlan, F. Ramaz, A. C. Boccara, and A. K. Dunn, “Heterodyne detection of multiply scattered monochromatic light with a multipixel detector,” Opt. Lett. 30(11), 1357–1359 (2005), http://www.opticsinfobase.org/abstract.cfm?URI=ol-30-11-1357 . [CrossRef] [PubMed]
  41. D. Ettori, K. Zarychta, E. Tinet, S. Avrillier, and J.-M. Tualle, “Time-resolved measurement of the scattered light with an interferometric method based on the use of a camera”, in Diffuse Optical Imaging of Tissue, R. Cubeddu ed., Vol. 6629 of Proceedings of SPIE-OSA Biomedical Optics (Optical Society of America, 2007), paper 6629_20.
  42. A. V. Oppenheim, R. W. Schafer, and J. R. Buck, Discrete-time signal processing” (Prentice-Hall signal processing series, ISBN 0–13–754920–2, 1999).
  43. M. Firbank, S. R. Arridge, M. Schweiger, and D. T. Delpy, “An investigation of light transport through scattering bodies with non-scattering regions,” Phys. Med. Biol. 41(4), 767–783 (1996). [CrossRef] [PubMed]
  44. T. Durduran, R. Choe, J. P. Culver, L. Zubkov, M. J. Holboke, J. Giammarco, B. Chance, and A. G. Yodh, “Bulk optical properties of healthy female breast tissue,” Phys. Med. Biol. 47(16), 2847–2861 (2002). [CrossRef] [PubMed]
  45. D. Grosenick, H. Wabnitz, K. T. Moesta, J. Mucke, P. M. Schlag, and H. Rinneberg, “Time-domain scanning optical mammography: II. Optical properties and tissue parameters of 87 carcinomas,” Phys. Med. Biol. 50(11), 2451–2468 (2005). [CrossRef] [PubMed]
  46. M. S. Patterson, B. Chance, and B. C. Wilson, “Time resolved reflectance and transmittance for the non-invasive measurement of tissue optical properties,” Appl. Opt. 28(12), 2331–2336 (1989). [CrossRef] [PubMed]
  47. R. Aronson, “Boundary conditions for diffusion of light,” J. Opt. Soc. Am. A 12(11), 2532–2539 (1995). [CrossRef]
  48. A. Pifferi, A. Torricelli, A. Bassi, P. Taroni, R. Cubeddu, H. Wabnitz, D. Grosenick, M. Möller, R. Macdonald, J. Swartling, T. Svensson, S. Andersson-Engels, R. L. P. van Veen, H. J. C. M. Sterenborg, J.-M. Tualle, H. L. Nghiem, S. Avrillier, M. Whelan, and H. Stamm, “Performance assessment of photon migration instruments: the MEDPHOT protocol,” Appl. Opt. 44(11), 2104–2114 (2005), http://www.opticsinfobase.org/abstract.cfm?URI=ao-44-11-2104 . [CrossRef] [PubMed]
  49. M. A. Webster, T. D. Gerke, A. M. Weiner, and K. J. Webb, “Spectral and temporal speckle field measurements of a random medium,” Opt. Lett. 29(13), 1491–1493 (2004), http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-29-13-1491 . [CrossRef] [PubMed]
  50. B. Varghese, V. Rajan, T. G. Van Leeuwen, and W. Steenbergen, “Path-length-resolved measurements of multiple scattered photons in static and dynamic turbid media using phase-modulated low-coherence interferometry,” J. Biomed. Opt. 12(2), 024020 (2007). [CrossRef] [PubMed]
  51. A. K. Dunn, H. Bolay, M. A. Moskowitz, and D. A. Boas, “Dynamic imaging of cerebral blood flow using laser speckle,” J. Cereb. Blood Flow Metab. 21(3), 195–201 (2001). [CrossRef] [PubMed]
  52. F. G. Santamaria, Photonic crystals based on silica microsphères”, Ph. D. thesis:Institude de Ciencia de Materials de Madride, (2003).
  53. http://www.me.rochester.edu/courses/ME241/SE3.html

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