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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 16 — Aug. 2, 2010
  • pp: 16640–16645

Efficient blue conversion from a 1064 nm microchip laser in long photonic crystal fiber tapers for fluorescence microscopy

A. Kudlinski, M. Lelek, B. Barviau, L. Audry, and A. Mussot  »View Author Affiliations


Optics Express, Vol. 18, Issue 16, pp. 16640-16645 (2010)
http://dx.doi.org/10.1364/OE.18.016640


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Abstract

Using a low-cost microchip laser and a long photonic crystal fiber taper, we report a supercontinuum source with a very efficient visible conversion, especially in the blue region (around 420 nm). About 30 % of the total average output power is located in the 350–600 nm band, which is of primary importance in a number of biophotonics applications such as flow cytometry or fluorescence imaging microscopy for instance. We successfully demonstrate the use of this visible-enhanced source for a three-color imaging of HeLa cells in wide-field microscopy.

© 2010 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(180.2520) Microscopy : Fluorescence microscopy
(190.4370) Nonlinear optics : Nonlinear optics, fibers
(190.5530) Nonlinear optics : Pulse propagation and temporal solitons
(060.5295) Fiber optics and optical communications : Photonic crystal fibers

ToC Category:
Nonlinear Optics

History
Original Manuscript: March 26, 2010
Revised Manuscript: April 20, 2010
Manuscript Accepted: April 21, 2010
Published: July 23, 2010

Virtual Issues
Vol. 5, Iss. 12 Virtual Journal for Biomedical Optics

Citation
A. Kudlinski, M. Lelek, B. Barviau, L. Audry, and A. Mussot, "Efficient blue conversion from a 1064 nm microchip laser in long photonic crystal fiber tapers for fluorescence microscopy," Opt. Express 18, 16640-16645 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-16-16640


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References

  1. See e. g. www.nktphotonics.com or www.fianium.com or www.leukos-systems.com
  2. www.leica-microsystems.com
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