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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 16 — Aug. 2, 2010
  • pp: 17209–17219

Fast image analysis in polarization SHG microscopy.

Ivan Amat-Roldan, Sotiris Psilodimitrakopoulos, Pablo Loza-Alvarez, and David Artigas  »View Author Affiliations

Optics Express, Vol. 18, Issue 16, pp. 17209-17219 (2010)

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Pixel resolution polarization-sensitive second harmonic generation (PSHG) imaging has been recently shown as a promising imaging modality, by largely enhancing the capabilities of conventional intensity-based SHG microscopy. PSHG is able to obtain structural information from the elementary SHG active structures, which play an important role in many biological processes. Although the technique is of major interest, acquiring such information requires long offline processing, even with current computers. In this paper, we present an approach based on Fourier analysis of the anisotropy signature that allows processing the PSHG images in less than a second in standard single core computers. This represents a temporal improvement of several orders of magnitude compared to conventional fitting algorithms. This opens up the possibility for fast PSHG information with the subsequent benefit of potential use in medical applications.

© 2010 OSA

OCIS Codes
(100.2960) Image processing : Image analysis
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: May 12, 2010
Revised Manuscript: July 8, 2010
Manuscript Accepted: July 15, 2010
Published: July 29, 2010

Virtual Issues
Vol. 5, Iss. 12 Virtual Journal for Biomedical Optics

Ivan Amat-Roldan, Sotiris Psilodimitrakopoulos, Pablo Loza-Alvarez, and David Artigas, "Fast image analysis in polarization SHG microscopy.," Opt. Express 18, 17209-17219 (2010)

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