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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 16 — Aug. 2, 2010
  • pp: 17382–17391

Miniaturized video-rate epi-third-harmonic-generation fiber-microscope

Shih-Hsuan Chia, Che-Hang Yu, Chih-Han Lin, Nai-Chia Cheng, Tzu-Ming Liu, Ming-Che Chan, I-Hsiu Chen, and Chi-Kuang Sun  »View Author Affiliations

Optics Express, Vol. 18, Issue 16, pp. 17382-17391 (2010)

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With a micro-electro-mechanical system (MEMS) mirror, we successfully developed a miniaturized epi-third-harmonic-generation (epi-THG) fiber-microscope with a video frame rate (31Hz), which was designed for in vivo optical biopsy of human skin. With a large-mode-area (LMA) photonic crystal fiber (PCF) and a regular microscopic objective, the nonlinear distortion of the ultrafast pulses delivery could be much reduced while still achieving a 0.4μm lateral resolution for epi-THG signals. In vivo real time virtual biopsy of the Asian skin with a video rate (31Hz) and a sub-micron resolution was obtained. The result indicates that this miniaturized system was compact enough for the least invasive hand-held clinical use.

© 2010 OSA

OCIS Codes
(190.2620) Nonlinear optics : Harmonic generation and mixing
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: April 26, 2010
Revised Manuscript: June 29, 2010
Manuscript Accepted: July 1, 2010
Published: July 30, 2010

Virtual Issues
Vol. 5, Iss. 12 Virtual Journal for Biomedical Optics

Shih-Hsuan Chia, Che-Hang Yu, Chih-Han Lin, Nai-Chia Cheng, Tzu-Ming Liu, Ming-Che Chan, I-Hsiu Chen, and Chi-Kuang Sun, "Miniaturized video-rate epi-third-harmonic-generation fiber-microscope," Opt. Express 18, 17382-17391 (2010)

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