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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 17 — Aug. 16, 2010
  • pp: 17983–17996

Phase contrast optical tweezers

Ali Mahmoudi and S. Nader S. Reihani  »View Author Affiliations

Optics Express, Vol. 18, Issue 17, pp. 17983-17996 (2010)

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In this paper, for the first time, we report on systematic theoretical and experimental investigation of Phase Contrast Optical Tweezers (PCOT) which could be an indispensable tool for micromanipulation of the transparent micro and nano objects such as biological tissues and vesicles. The quadrant photodiode detection scheme and the power-spectrum calibration method is shown to be valid for this case. We have shown that the phase objective with new designed phase plates can provide nearly aberration-free condition at a desired depth. This could be a valuable advantage for simultaneous in-depth micro-manipulations and visualization of the sample.

© 2010 Optical Society of America

OCIS Codes
(000.3110) General : Instruments, apparatus, and components common to the sciences
(140.7010) Lasers and laser optics : Laser trapping
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Optical Trapping and Manipulation

Original Manuscript: March 23, 2010
Revised Manuscript: April 17, 2010
Manuscript Accepted: April 19, 2010
Published: August 6, 2010

Virtual Issues
Vol. 5, Iss. 13 Virtual Journal for Biomedical Optics

Ali Mahmoudi and S. Nader S. Reihani, "Phase contrast optical tweezers," Opt. Express 18, 17983-17996 (2010)

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  1. A. Ashkin, and J. M. Dziedzic, “Optical trapping and manipulation of viruses and bacteria,” Science 235, 1517–1520 (1987). [CrossRef] [PubMed]
  2. C. Bustamante, Z. Bryant, and S. B. Smith, “Ten years of tension: single-molecule DNA mechanics,” Nature 421, 423–427 (2003). [CrossRef] [PubMed]
  3. S. M. Block, D. F. Blair, and H. C. Berg, “Compliance of bacterial flagella measured with optical tweezers,” Nature 338, 514–518 (1989). [CrossRef] [PubMed]
  4. T. M. Hansen, S. N. S. Reihani, L. B. Oddershede, and M. A. Sorensen, “Correlation between mechanical strength of messenger RNA pseudoknots and ribosomal frame shifting,” Proc. Natl. Acad. Sci. U.S.A. 104, 5830–5835 (2007). [CrossRef] [PubMed]
  5. R. Agarwal, K. Ladavac, Y. Roichman, G. Yu, C. M. Lieber, and D. G. Grier, “Manipulation and assembly of nanowires with holographic optical traps,” Opt. Express 13, 8906–8912 (2005). [CrossRef] [PubMed]
  6. S. Tan, H. A. Lopez, C. W. Cai, and Y. Zhang, “Optical Trapping of Single-Walled Carbon Nanotubes,” Nano Lett. 4, 1415–1419 (2004). [CrossRef]
  7. C. Selhuber-Unkel, I. Zins, O. Schubert, C. Sönnichsen, and L. B. Oddershede, “Quantitative Optical Trapping of Single Gold Nanorods,” Nano Lett. 8(9), 2998–3003 (2008). [CrossRef]
  8. Y. Seol, A. E. Carpenter, and T. T. Perkins, “Gold nanoparticles: enhanced optical trapping and sensitivity coupled with significant heating,” Opt. Lett. 31, 2429–2431 (2006). [CrossRef] [PubMed]
  9. F. Hajizadeh, and S. N. S. Reihani, “Optimized optical trapping of gold nanoparticles,” Opt. Express 18, 551–559 (2010). [CrossRef] [PubMed]
  10. A. Ashkin, J. M. Dziedzic, J. E. Bjorkholm, and S. Chu, “Observation of a single-beam gradient force optical trap for dielectric particles,” Opt. Lett. 11, 288–290 (1986). [CrossRef] [PubMed]
  11. M. Capitanio, G. Romano, R. Ballerini, M. Giuntini, F. S. Pavone, D. Dunlap, and L. Finzi, “Calibration of optical tweezers with differential interference contrast signals,” Rev. Sci. Instrum. 73, 1687 (2002). [CrossRef]
  12. R. Wayne, Light and video microscopy (Elsevier, 2009).
  13. http://microscopyu.com
  14. F. Zernike, “Phase-contrast, a new method for the microscopic observation of transparent objects,” Physica 9(Part I), 689–698 (1942a).
  15. F. Zernike, “Phase-contrast, a new method for the microscopic observation of transparent objects,” Physica 9(Part II), 974–986 (1942b).
  16. R. Dimova, S. Aranda, N. Bezlyepkina, V. Nikolov, K. A. Riske, and R. Lipowsky, “A practical guide to giant vesicles. Probing the membrane nanoregime via optical microscopy,” J. Phys. Condens. Matter 18, S1151 (2006).
  17. F. Gittes, and C. F. Schmidt, “Interference model for back-focal plane displacement detection in optical tweezers,” Opt. Lett. 23, 7–9 (1998). [CrossRef]
  18. K. B. Sørensen, and H. Flyvbjerg, “Power spectrum analysis for optical tweezers,” Rev. Sci. Instrum. 75, 594–612 (2004). [CrossRef]
  19. N. B. Viana, M. S. Rocha, O. N. Mesquita, A. Mazolli, and P. A. Maia Neto, “Characterization of objective transmittance for optical tweezers,” Appl. Opt. 45, 4263–4269 (2006). [CrossRef]
  20. E. Schäffer, S. F. Nørrelykke, and J. Howard, “Surface Forces and Drag Coefficients of Microspheres near a Plane Surface Measured with Optical Tweezers,” Langmuir 23, 3654–3665 (2007). [CrossRef] [PubMed]
  21. E. J. G. Peterman, F. Gittes, and C. F. Schmidt, “Laser-Induced Heating in Optical Traps,” Biophys. J. 84, 1308–1316 (2003). [CrossRef] [PubMed]
  22. J. K. Dreyer, K. B. Sørensen, and L. Oddershede, “Improved axial position detection in optical tweezers measurements,” Appl. Opt. 43, 1991–1995 (2004). [CrossRef] [PubMed]
  23. S. N. S. Reihani, and B. Lene, “Oddershede, “Optimizing immersion media refractive index improves optical trapping by compensating spherical aberrations,” Opt. Lett. 32, 1998–2000 (2007). [CrossRef] [PubMed]
  24. N. B. Simpson, D. McGloin, K. Dholakia, L. Allen, and M. J. Padgett, “Optical tweezers with increased axial trapping efficiency,” J. Mod. Opt. 45, 1943–1949 (1998). [CrossRef]
  25. P. C. Ke, and M. Gu, “Characterization of trapping force in the presence of spherical aberration,” J. Mod. Opt. 45, 2159–2168 (1998). [CrossRef]
  26. A. Samadi and S. N. S. Reihani, “Optimal beam diameter for optical tweezers,” (Accepted for publication in Opt. Lett., Manuscript ID: 120249).
  27. P. Török, P. Varga, Z. Laczik, and G. R. Booker, “Electromagnetic diffraction of light focused through a planar interface between materials of mismatched refractive indices: an integral representation,” J. Opt. Soc. Am. A 12, 325–332 (1995). [CrossRef]
  28. J. Marvin Weber, Handbook of optical materials (CRC Press, 2002).

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