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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 18 — Aug. 30, 2010
  • pp: 18519–18524

Spectroscopic photoacoustic microscopy using a photonic crystal fiber supercontinuum source

Yazan N. Billeh, Mengyang Liu, and Takashi Buma  »View Author Affiliations


Optics Express, Vol. 18, Issue 18, pp. 18519-18524 (2010)
http://dx.doi.org/10.1364/OE.18.018519


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Abstract

Photoacoustic microscopy (PAM) provides high resolution images with excellent image contrast based on optical absorption. The compact size and high repetition rate of pulsed microchip lasers make them attractive sources for PAM. However, their fixed wavelength output precludes their use in spectroscopic PAM. We are developing a tunable optical source based on a microchip laser that is suitable for spectroscopic PAM. Pulses from a 6.6 kHz repetition rate Q-switched Nd:YAG microchip laser are sent through a photonic crystal fiber with a zero dispersion wavelength at 1040 nm. The highly nonlinear optical propagation produces a supercontinuum spectrum spanning 500 – 1300 nm. A tunable band pass filter selects the desired wavelength band from the supercontinuum. Our PAM system employs optical focusing and a 25 MHz spherically focused detection transducer. En-face imaging experiments were performed at seven different wavelengths from 575 to 875 nm. A simple discriminant analysis of the multiwavelength photoacoustic data produces images that clearly distinguish the different absorbing regions of ink phantoms. These results suggest the potential of this compact tunable source for spectroscopic photoacoustic microscopy.

© 2010 OSA

OCIS Codes
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(110.4234) Imaging systems : Multispectral and hyperspectral imaging
(320.6629) Ultrafast optics : Supercontinuum generation

ToC Category:
Microscopy

History
Original Manuscript: May 17, 2010
Revised Manuscript: July 31, 2010
Manuscript Accepted: August 9, 2010
Published: August 16, 2010

Virtual Issues
Vol. 5, Iss. 13 Virtual Journal for Biomedical Optics

Citation
Yazan N. Billeh, Mengyang Liu, and Takashi Buma, "Spectroscopic photoacoustic microscopy using a photonic crystal fiber supercontinuum source," Opt. Express 18, 18519-18524 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-18-18519


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