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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 18 — Aug. 30, 2010
  • pp: 18720–18731

Simultaneous two-photon imaging and photo-stimulation with structured light illumination

Marco Dal Maschio, Francesco Difato, Riccardo Beltramo, Axel Blau, Fabio Benfenati, and Tommaso Fellin  »View Author Affiliations


Optics Express, Vol. 18, Issue 18, pp. 18720-18731 (2010)
http://dx.doi.org/10.1364/OE.18.018720


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Abstract

Holographic microscopy is increasingly recognized as a promising tool for the study of the central nervous system. Here we present a “holographic module”, a simple optical path that can be combined with commercial scanheads for simultaneous imaging and uncaging with structured two-photon light. The present microscope is coupled to two independently tunable lasers and has two principal configurations: holographic imaging combined with galvo-steered uncaging and holographic uncaging combined with conventional scanning imaging. We applied this flexible system for simultaneous two-photon imaging and photostimulation of neuronal cells with complex light patterns, opening new perspectives for the study of brain function in situ and in vivo.

© 2010 OSA

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(090.1995) Holography : Digital holography
(180.4315) Microscopy : Nonlinear microscopy
(070.6120) Fourier optics and signal processing : Spatial light modulators

ToC Category:
Microscopy

History
Original Manuscript: May 26, 2010
Revised Manuscript: July 29, 2010
Manuscript Accepted: August 7, 2010
Published: August 18, 2010

Virtual Issues
Vol. 5, Iss. 13 Virtual Journal for Biomedical Optics

Citation
Marco Dal Maschio, Francesco Difato, Riccardo Beltramo, Axel Blau, Fabio Benfenati, and Tommaso Fellin, "Simultaneous two-photon imaging and photo-stimulation with structured light illumination," Opt. Express 18, 18720-18731 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-18-18720


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