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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 18 — Aug. 30, 2010
  • pp: 18875–18885

Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI)

Thomas Dertinger, Ryan Colyer, Robert Vogel, Jörg Enderlein, and Shimon Weiss  »View Author Affiliations

Optics Express, Vol. 18, Issue 18, pp. 18875-18885 (2010)

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uperresolution Optical Fluctuation Imaging (SOFI) as initially demonstrated allows for a resolution enhancement in imaging by a factor of square-root of two. Here, we demonstrate how to increase the resolution of SOFI images by re-weighting the Optical Transfer Function (OTF). Furthermore, we demonstrate how cross-cumulants can be exploited to obtain a fair approximation of the underlying Point-Spread Function. We show a two-fold increase of resolution (over the diffraction limit) of near-infrared quantum dot labeled tubulin-network of 3T3 fibroblasts

© 2010 OSA

OCIS Codes
(100.6640) Image processing : Superresolution
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:

Original Manuscript: June 18, 2010
Revised Manuscript: July 18, 2010
Manuscript Accepted: July 21, 2010
Published: August 19, 2010

Virtual Issues
Vol. 5, Iss. 13 Virtual Journal for Biomedical Optics

Thomas Dertinger, Ryan Colyer, Robert Vogel, Jörg Enderlein, and Shimon Weiss, "Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI)," Opt. Express 18, 18875-18885 (2010)

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