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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 18 — Aug. 30, 2010
  • pp: 19232–19241

Structured light sheet fluorescence microscopy based on four beam interference

Ming Lei and Andreas Zumbusch  »View Author Affiliations


Optics Express, Vol. 18, Issue 18, pp. 19232-19241 (2010)
http://dx.doi.org/10.1364/OE.18.019232


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Abstract

A 3D structured light sheet microscope using a four-faceted symmetric pyramid is presented. The sample is illuminated by the resulting four beam interference field. This approach combines advantages of standing wave and structured illumination microscopy. Examples of micrographs of fluorescently labeled Chinese hamster ovary (CHO) cells as well as of the compound eyes of drosophila are shown and the optical sectioning ability of our system is demonstrated. The capabilities and the limitations of the scheme are discussed.

© 2010 OSA

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(260.3160) Physical optics : Interference

ToC Category:
Microscopy

History
Original Manuscript: July 21, 2010
Revised Manuscript: August 13, 2010
Manuscript Accepted: August 16, 2010
Published: August 25, 2010

Virtual Issues
Vol. 5, Iss. 13 Virtual Journal for Biomedical Optics

Citation
Ming Lei and Andreas Zumbusch, "Structured light sheet fluorescence microscopy based on four beam interference," Opt. Express 18, 19232-19241 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-18-19232


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