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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 18 — Aug. 30, 2010
  • pp: 19263–19272

A method for achieving super-resolved widefield CARS microscopy

Kim M. Hajek, Brad Littleton, Douglas Turk, Timothy J. McIntyre, and Halina Rubinsztein-Dunlop  »View Author Affiliations


Optics Express, Vol. 18, Issue 18, pp. 19263-19272 (2010)
http://dx.doi.org/10.1364/OE.18.019263


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Abstract

We propose a scheme for achieving widefield coherent anti-Stokes Raman scattering (CARS) microscopy images with sub-diffraction-limited resolution. This approach adds structured illumination to the widefield CARS configuration [Applied Physics Letters 84, 816 (2004)]. By capturing a number of images at different phases of the standing wave pattern, an image with up to three times the resolution of the original can be constructed. We develop a theoretical treatment of this system and perform numerical simulations for a typical CARS system, which indicate that resolutions around 120 nm are obtainable with the present scheme. As an imaging system, this method combines the advantages of sub-diffraction-limited resolution, endogenous contrast generation, and a wide field of view.

© 2010 OSA

OCIS Codes
(110.0180) Imaging systems : Microscopy
(120.4120) Instrumentation, measurement, and metrology : Moire' techniques
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(330.6130) Vision, color, and visual optics : Spatial resolution

ToC Category:
Microscopy

History
Original Manuscript: June 1, 2010
Revised Manuscript: August 5, 2010
Manuscript Accepted: August 11, 2010
Published: August 26, 2010

Virtual Issues
Vol. 5, Iss. 13 Virtual Journal for Biomedical Optics

Citation
Kim M. Hajek, Brad Littleton, Douglas Turk, Timothy J. McIntyre, and Halina Rubinsztein-Dunlop, "A method for achieving super-resolved widefield CARS microscopy," Opt. Express 18, 19263-19272 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-18-19263


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