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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 19 — Sep. 13, 2010
  • pp: 20049–20058

Development of a time-gated system for Raman spectroscopy of biological samples

Florian Knorr, Zachary J. Smith, and Sebastian Wachsmann-Hogiu  »View Author Affiliations

Optics Express, Vol. 18, Issue 19, pp. 20049-20058 (2010)

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A time gating system has been constructed that is capable of recording high quality Raman spectra of highly fluorescing biological samples while operating below the photodamage threshold. Using a collinear gating geometry and careful attention to power conservation, we have achieved all-optical switching with a one picosecond gating time and 5% peak gating efficiency. The energy per pulse in this instrument is more than 3 orders of magnitude weaker than previous reports. Using this system we have performed proof-of-concept experiments on a sample composed of perylene dissolved in toluene, and the stem of a Jasminum multiflorum plant, the latter case being particularly important for the study of plants used in production of cellulosic biofuels. In both cases, a high SNR spectrum of the high-wavenumber region of the spectrum was recorded in the presence of an overwhelming fluorescence background.

© 2010 Optical Society of America

OCIS Codes
(120.6200) Instrumentation, measurement, and metrology : Spectrometers and spectroscopic instrumentation
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(190.3270) Nonlinear optics : Kerr effect

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: August 9, 2010
Revised Manuscript: August 30, 2010
Manuscript Accepted: August 31, 2010
Published: September 3, 2010

Virtual Issues
Vol. 5, Iss. 13 Virtual Journal for Biomedical Optics

Florian Knorr, Zachary J. Smith, and Sebastian Wachsmann-Hogiu, "Development of a time-gated system for Raman spectroscopy of biological samples," Opt. Express 18, 20049-20058 (2010)

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