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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 19 — Sep. 13, 2010
  • pp: 20125–20135

Study of cell-biosubstrate contacts via surface plasmon polariton phase microscopy

Yuan-Deng Su, Kuo-Chih Chiu, Nan-Shan Chang, Hua-Lin Wu, and Shean-Jen Chen  »View Author Affiliations


Optics Express, Vol. 18, Issue 19, pp. 20125-20135 (2010)
http://dx.doi.org/10.1364/OE.18.020125


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Abstract

This study utilized a developed surface plasmon polariton (SPP) phase microscopy to observe cell-biosubstrate contacts. The developed SPP phase microscopy is highly sensitive to cell membrane contact with biosubstrates and also provides long-term phase stability to achieve time-lapse living cell observation. As such, an SPP intensity and phase sensitivity comparison demonstrates that the sensitivity of the phase measurement can be 100-fold greater than that of the intensity measurement. Also, a more than 2-hour cell apoptosis observation via the SPP phase microscopy is presented. To implement the incident angle from 70° to 78°, cell-biosubstrate contact images corresponding to the surface plasmon resonance (SPR) angles are obtained by utilizing the SPP phase measurement. According to the information of the corresponding SPR angle image and a multilayer simulation, the contact distances between a living melanoma cell and a bovine serum albumin substrate at four different locations have been estimated.

© 2010 Optical Society of America

OCIS Codes
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.1530) Medical optics and biotechnology : Cell analysis
(240.6680) Optics at surfaces : Surface plasmons

ToC Category:
Optics at Surfaces

History
Original Manuscript: June 28, 2010
Revised Manuscript: September 1, 2010
Manuscript Accepted: September 1, 2010
Published: September 7, 2010

Virtual Issues
Vol. 5, Iss. 13 Virtual Journal for Biomedical Optics

Citation
Yuan-Deng Su, Kuo-Chih Chiu, Nan-Shan Chang, Hua-Lin Wu, and Shean-Jen Chen, "Study of cell-biosubstrate contacts via surface plasmon polariton phase microscopy," Opt. Express 18, 20125-20135 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-19-20125


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