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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 19 — Sep. 13, 2010
  • pp: 20263–20272

Three-dimensional nano-localization of single fluorescent emitters

Iwan Märki, Noelia L. Bocchio, Stefan Geissbuehler, François Aguet, Alberto Bilenca, and Theo Lasser  »View Author Affiliations


Optics Express, Vol. 18, Issue 19, pp. 20263-20272 (2010)
http://dx.doi.org/10.1364/OE.18.020263


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Abstract

We present a combination of self-interference microscopy with lateral super-resolution microscopy and introduce a novel approach for localizing a single nano-emitter to within a few nanometers in all three dimensions over a large axial range. We demonstrate nanometer displacements of quantum dots placed on top of polymer bilayers that undergo swelling when changing from an air to a water environment, achieving standard deviations below 10 nm for axial and lateral localization.

© 2010 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:
Microscopy

History
Original Manuscript: June 29, 2010
Revised Manuscript: September 1, 2010
Manuscript Accepted: September 2, 2010
Published: September 8, 2010

Virtual Issues
Vol. 5, Iss. 13 Virtual Journal for Biomedical Optics

Citation
Iwan Märki, Noelia L. Bocchio, Stefan Geissbuehler, François Aguet, Alberto Bilenca, and Theo Lasser, "Three-dimensional nano-localization of single fluorescent emitters," Opt. Express 18, 20263-20272 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-19-20263


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