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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 19 — Sep. 13, 2010
  • pp: 20505–20511

Supercontinuum source tuned by an on-axis monochromator for fluorescence lifetime imaging

Raffaella Mercatelli, Silvia Soria, Giuseppe Molesini, Federica Bianco, Giancarlo Righini, and Franco Quercioli  »View Author Affiliations


Optics Express, Vol. 18, Issue 19, pp. 20505-20511 (2010)
http://dx.doi.org/10.1364/OE.18.020505


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Abstract

We report on the application of an optically tunable coherent white light source, based on the supercontinuum generation from microstructured optical fibres, to fluorescence lifetime imaging (FLIM) microscopy and Foerster resonance energy transfer (FRET). A prototype lens based on monotonic longitudinal chromatic aberration is used for tuning the supercontinuum wavelengths from 400 to 1000 nm and acts as an axial monochromator, suitable for fibre delivery in confocal microscopy.

© 2010 OSA

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(220.3620) Optical design and fabrication : Lens system design

ToC Category:
Microscopy

History
Original Manuscript: July 22, 2010
Revised Manuscript: August 26, 2010
Manuscript Accepted: August 28, 2010
Published: September 10, 2010

Virtual Issues
Vol. 5, Iss. 13 Virtual Journal for Biomedical Optics

Citation
Raffaella Mercatelli, Silvia Soria, Giuseppe Molesini, Federica Bianco, Giancarlo Righini, and Franco Quercioli, "Supercontinuum source tuned by an on-axis monochromator for fluorescence lifetime imaging," Opt. Express 18, 20505-20511 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-19-20505


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