OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 19 — Sep. 13, 2010
  • pp: 20505–20511

Supercontinuum source tuned by an on-axis monochromator for fluorescence lifetime imaging

Raffaella Mercatelli, Silvia Soria, Giuseppe Molesini, Federica Bianco, Giancarlo Righini, and Franco Quercioli  »View Author Affiliations

Optics Express, Vol. 18, Issue 19, pp. 20505-20511 (2010)

View Full Text Article

Enhanced HTML    Acrobat PDF (1047 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We report on the application of an optically tunable coherent white light source, based on the supercontinuum generation from microstructured optical fibres, to fluorescence lifetime imaging (FLIM) microscopy and Foerster resonance energy transfer (FRET). A prototype lens based on monotonic longitudinal chromatic aberration is used for tuning the supercontinuum wavelengths from 400 to 1000 nm and acts as an axial monochromator, suitable for fibre delivery in confocal microscopy.

© 2010 OSA

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(220.3620) Optical design and fabrication : Lens system design

ToC Category:

Original Manuscript: July 22, 2010
Revised Manuscript: August 26, 2010
Manuscript Accepted: August 28, 2010
Published: September 10, 2010

Virtual Issues
Vol. 5, Iss. 13 Virtual Journal for Biomedical Optics

Raffaella Mercatelli, Silvia Soria, Giuseppe Molesini, Federica Bianco, Giancarlo Righini, and Franco Quercioli, "Supercontinuum source tuned by an on-axis monochromator for fluorescence lifetime imaging," Opt. Express 18, 20505-20511 (2010)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. J. R. Lakowicz, Principles of Fluorescence Spectroscopy, 3rd Edition (Springer, 2006).
  2. E. A. Jares-Erijman and T. M. Jovin, “Imaging molecular interactions in living cells by FRET microscopy,” Curr. Opin. Chem. Biol. 10(5), 409–416 (2006). [CrossRef] [PubMed]
  3. D. M. Owen, E. Auksorius, H. B. Manning, C. B. Talbot, P. A. A. de Beule, C. Dunsby, M. A. A. Neil, and P. M. W. French, “Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source,” Opt. Lett. 32(23), 3408–3410 (2007). [CrossRef] [PubMed]
  4. G. McConnell, “Confocal laser scanning fluorescence microscopy with a visible continuum source,” Opt. Express 12(13), 2844–2850 (2004). [CrossRef] [PubMed]
  5. H. N. Paulsen, K. M. Hilligsøe, J. Thøgersen, S. R. Keiding, and J. J. Larsen, “Coherent anti-Stokes Raman scattering microscopy with a photonic crystal fiber based light source,” Opt. Lett. 28(13), 1123–1125 (2003). [CrossRef] [PubMed]
  6. J. A. Palero, V. O. Boer, J. C. Vijverberg, H. C. Gerritsen, and H. J. C. M. Sterenborg, “Short-wavelength two-photon excitation fluorescence microscopy of tryptophan with a photonic crystal fiber based light source,” Opt. Express 13(14), 5363–5368 (2005). [CrossRef] [PubMed]
  7. C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004). [CrossRef]
  8. D. M. Grant, J. McGinty, E. J. McGhee, T. D. Bunney, D. M. Owen, C. B. Talbot, W. Zhang, S. Kumar, I. Munro, P. M. P. Lanigan, G. T. Kennedy, C. Dunsby, A. I. Magee, P. Courtney, M. Katan, M. A. A. Neil, and P. M. W. French, “High speed optically sectioned fluorescence lifetime imaging permits study of live cell signaling events,” Opt. Express 15(24), 15656–15673 (2007). [CrossRef] [PubMed]
  9. P. Blandin, S. Lévêque-Fort, S. Lécart, J. C. Cossec, M.-C. Potier, Z. Lenkei, F. Druon, and P. Georges, “Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source,” Appl. Opt. 48(3), 553–559 (2009). [CrossRef] [PubMed]
  10. A. Gurtler, J. Gilijamse, A. Wetzels, L. Noordam, E. Sali, and M. Bellini, “Frequency selection of supercontinuum ultrashort pulses using a Fresnel zone plate,” Opt. Commun. 270(2), 336–339 (2007). [CrossRef]
  11. K. Shi, P. Li, S. Yin, and Z. Liu, “Chromatic confocal microscopy using supercontinuum light,” Opt. Express 12(10), 2096–2101 (2004). [CrossRef] [PubMed]
  12. G. McConnell, S. Poland, and J. M. Girkin, “Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy,” Rev. Sci. Instrum. 77(1), 013702 (2006). [CrossRef]
  13. F. Quercioli, B. Tiribilli, and G. Molesini, “Optical Surface profile transducer,” Opt. Eng. 27, 135–142 (1988).
  14. B. J. Bacskai, J. Skoch, G. A. Hickey, R. Allen, and B. T. Hyman, “Fluorescence resonance energy transfer determinations using multiphoton fluorescence lifetime imaging microscopy to characterize amyloid-beta plaques,” J. Biomed. Opt. 8(3), 368–375 (2003). [CrossRef] [PubMed]
  15. C. Biskup, L. Kelbauskas, T. Zimmer, K. Benndorf, A. Bergmann, W. Becker, J. P. Ruppersberg, C. Stockklausner, and N. Klöcker, “Interaction of PSD-95 with potassium channels visualized by fluorescence lifetime-based resonance energy transfer imaging,” J. Biomed. Opt. 9(4), 753–759 (2004). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3
Fig. 4 Fig. 5

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited