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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 2 — Jan. 18, 2010
  • pp: 877–884

Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy

Paul A. Dalgarno, Heather I. C. Dalgarno, Aurélie Putoud, Robert Lambert, Lynn Paterson, David C. Logan, David P. Towers, Richard J. Warburton, and Alan H. Greenaway  »View Author Affiliations

Optics Express, Vol. 18, Issue 2, pp. 877-884 (2010)

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A conventional microscope produces a sharp image from just a single object-plane. This is often a limitation, notably in cell biology. We present a microscope attachment which records sharp images from several object-planes simultaneously. The key concept is to introduce a distorted diffraction grating into the optical system, establishing an order-dependent focussing power in order to generate several images, each arising from a different object-plane. We exploit this multiplane imaging not just for bio-imaging but also for nano-particle tracking, achieving ~10 nm z position resolution by parameterising the images with an image sharpness metric.

© 2010 OSA

OCIS Codes
(050.0050) Diffraction and gratings : Diffraction and gratings
(050.1950) Diffraction and gratings : Diffraction gratings
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.0180) Microscopy : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: October 23, 2009
Revised Manuscript: December 17, 2009
Manuscript Accepted: December 21, 2009
Published: January 6, 2010

Virtual Issues
Vol. 5, Iss. 3 Virtual Journal for Biomedical Optics

Paul A. Dalgarno, Heather I. C. Dalgarno, Aurélie Putoud, Robert Lambert, Lynn Paterson, David C. Logan, David P. Towers, Richard J. Warburton, and Alan H. Greenaway, "Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy," Opt. Express 18, 877-884 (2010)

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