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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 2 — Jan. 18, 2010
  • pp: 938–946

Image patterned molecular delivery into live cells using gold particle coated substrates

Ting-Hsiang Wu, Sheraz Kalim, Caitlin Callahan, Michael A. Teitell, and Pei-Yu Chiou  »View Author Affiliations

Optics Express, Vol. 18, Issue 2, pp. 938-946 (2010)

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An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein. Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.

© 2010 OSA

OCIS Codes
(140.3540) Lasers and laser optics : Lasers, Q-switched
(170.1420) Medical optics and biotechnology : Biology
(170.1530) Medical optics and biotechnology : Cell analysis

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 4, 2009
Revised Manuscript: October 30, 2009
Manuscript Accepted: November 4, 2009
Published: January 7, 2010

Virtual Issues
Vol. 5, Iss. 3 Virtual Journal for Biomedical Optics

Ting-Hsiang Wu, Sheraz Kalim, Caitlin Callahan, Michael A. Teitell, and Pei-Yu Chiou, "Image patterned molecular delivery into live cells using gold particle coated substrates," Opt. Express 18, 938-946 (2010)

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