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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 2 — Jan. 18, 2010
  • pp: 988–999

Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence

Chunqiang Li, Riikka K. Pastila, Costas Pitsillides, Judith M. Runnels, Mehron Puoris’haag, Daniel Côté, and Charles P. Lin  »View Author Affiliations


Optics Express, Vol. 18, Issue 2, pp. 988-999 (2010)
http://dx.doi.org/10.1364/OE.18.000988


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Abstract

We describe a new method for imaging leukocytes in vivo by exciting the endogenous protein fluorescence in the ultraviolet (UV) spectral region where tryptophan is the major fluorophore. Two-photon excitation near 590 nm allows noninvasive optical sectioning through the epidermal cell layers into the dermis of mouse skin, where leukocytes can be observed by video-rate microscopy to interact dynamically with the dermal vascular endothelium. Inflammation significantly enhances leukocyte rolling, adhesion, and tissue infiltration. After exiting the vasculature, leukocytes continue to move actively in tissue as observed by time-lapse microscopy, and are distinguishable from resident autofluorescent cells that are not motile. Because the new method alleviates the need to introduce exogenous labels, it is potentially applicable for tracking leukocytes and monitoring inflammatory cellular reactions in humans.

© 2010 OSA

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: October 21, 2009
Revised Manuscript: December 13, 2009
Manuscript Accepted: December 21, 2009
Published: January 7, 2010

Virtual Issues
Vol. 5, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Chunqiang Li, Riikka K. Pastila, Costas Pitsillides, Judith M. Runnels, Mehron Puoris’haag, Daniel Côté, and Charles P. Lin, "Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence," Opt. Express 18, 988-999 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-2-988


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