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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 22 — Oct. 25, 2010
  • pp: 22722–22733

Real-time particle tracking at 10,000 fps using optical fiber illumination

Oliver Otto, Fabian Czerwinski, Joanne L. Gornall, Gunter Stober, Lene B. Oddershede, Ralf Seidel, and Ulrich F. Keyser  »View Author Affiliations

Optics Express, Vol. 18, Issue 22, pp. 22722-22733 (2010)

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We introduce optical fiber illumination for real-time tracking of optically trapped micrometer-sized particles with microsecond time resolution. Our light source is a high-radiance mercury arc lamp and a 600μm optical fiber for short-distance illumination of the sample cell. Particle tracking is carried out with a software implemented cross-correlation algorithm following image acquisition from a CMOS camera. Our image data reveals that fiber illumination results in a signal-to-noise ratio usually one order of magnitude higher compared to standard Köhler illumination. We demonstrate position determination of a single optically trapped colloid with up to 10,000 frames per second over hours. We calibrate our optical tweezers and compare the results with quadrant photo diode measurements. Finally, we determine the positional accuracy of our setup to 2 nm by calculating the Allan variance. Our results show that neither illumination nor software algorithms limit the speed of real-time particle tracking with CMOS technology.

© 2010 Optical Society of America

OCIS Codes
(000.3110) General : Instruments, apparatus, and components common to the sciences
(060.2310) Fiber optics and optical communications : Fiber optics
(110.4280) Imaging systems : Noise in imaging systems
(120.4800) Instrumentation, measurement, and metrology : Optical standards and testing
(350.4800) Other areas of optics : Optical standards and testing
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Instrumentation, Measurement, and Metrology

Original Manuscript: August 9, 2010
Revised Manuscript: September 30, 2010
Manuscript Accepted: October 1, 2010
Published: October 12, 2010

Oliver Otto, Fabian Czerwinski, Joanne L. Gornall, Gunter Stober, Lene B. Oddershede, Ralf Seidel, and Ulrich F. Keyser, "Real-time particle tracking at 10,000 fps using optical fiber illumination," Opt. Express 18, 22722-22733 (2010)

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