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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 23 — Nov. 8, 2010
  • pp: 23796–23804

Miniaturized multimodal CARS microscope based on MEMS scanning and a single laser source

Sangeeta Murugkar, Brett Smith, Prateek Srivastava, Adrian Moica, Majid Naji, Craig Brideau, Peter K. Stys, and Hanan Anis  »View Author Affiliations


Optics Express, Vol. 18, Issue 23, pp. 23796-23804 (2010)
http://dx.doi.org/10.1364/OE.18.023796


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Abstract

We demonstrate a novel miniaturized multimodal coherent anti-Stokes Raman scattering (CARS) microscope based on microelectromechanical systems (MEMS) scanning mirrors and custom miniature optics. A single Ti:sapphire femtosecond pulsed laser is used as the light source to produce the CARS, two photon excitation fluorescence (TPEF) and second harmonic generation (SHG) images using this miniaturized microscope. The high resolution and distortion-free images obtained from various samples such as a USAF target, fluorescent and polystyrene microspheres and biological tissue successfully demonstrate proof of concept, and pave the path towards future integration of parts into a handheld multimodal CARS probe for non- or minimally-invasive in vivo imaging.

© 2010 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(190.4180) Nonlinear optics : Multiphoton processes
(180.4315) Microscopy : Nonlinear microscopy
(230.4685) Optical devices : Optical microelectromechanical devices

ToC Category:
Microscopy

History
Original Manuscript: September 20, 2010
Revised Manuscript: October 21, 2010
Manuscript Accepted: October 21, 2010
Published: October 27, 2010

Virtual Issues
Vol. 6, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Sangeeta Murugkar, Brett Smith, Prateek Srivastava, Adrian Moica, Majid Naji, Craig Brideau, Peter K. Stys, and Hanan Anis, "Miniaturized multimodal CARS microscope based on MEMS scanning and a single laser source," Opt. Express 18, 23796-23804 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-23-23796


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