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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 23 — Nov. 8, 2010
  • pp: 24101–24108

MEMS scanner enabled real-time depth sensitive hyperspectral imaging of biological tissue

Youmin Wang, Sheldon Bish, James W Tunnell, and Xiaojing Zhang  »View Author Affiliations

Optics Express, Vol. 18, Issue 23, pp. 24101-24108 (2010)

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We demonstrate a hyperspectral and depth sensitive diffuse optical imaging microsystem, where fast scanning is provided by a CMOS compatible 2-axis MEMS mirror. By using lissajous scanning patterns, large field-of-view (FOV) of 1.2cm x 1.2cm images with lateral resolution of 100µm can be taken at 1.3 frames-per-second (fps). Hyperspectral and depth-sensitive images were acquired on tissue simulating phantom samples containing quantum dots (QDs) patterned at various depths in Polydimethylsiloxane (PDMS). Device performance delivers 6 nm spectral resolution and 0.43 wavelengths per second acquisition speed. A sample of porcine epithelium with subcutaneously placed QDs was also imaged. Images of the biological sample were processed by spectral unmixing in order to qualitatively separate chromophores in the final images and demonstrate spectral performance of the imaging system.

© 2010 OSA

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(300.2530) Spectroscopy : Fluorescence, laser-induced
(110.0113) Imaging systems : Imaging through turbid media
(230.4685) Optical devices : Optical microelectromechanical devices

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: August 27, 2010
Revised Manuscript: October 18, 2010
Manuscript Accepted: October 22, 2010
Published: November 3, 2010

Virtual Issues
Vol. 6, Iss. 1 Virtual Journal for Biomedical Optics

Youmin Wang, Sheldon Bish, James W Tunnell, and Xiaojing Zhang, "MEMS scanner enabled real-time depth sensitive hyperspectral imaging of biological tissue," Opt. Express 18, 24101-24108 (2010)

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