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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 24 — Nov. 22, 2010
  • pp: 24441–24450

Experimental Cerenkov luminescence tomography of the mouse model with SPECT imaging validation

Zhenhua Hu, Jimin Liang, Weidong Yang, Weiwei Fan, Congye Li, Xiaowei Ma, Xueli Chen, Xiaopeng Ma, Xiangsi Li, Xiaochao Qu, Jing Wang, Feng Cao, and Jie Tian  »View Author Affiliations

Optics Express, Vol. 18, Issue 24, pp. 24441-24450 (2010)

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Optical molecular imaging resulting from Cerenkov radiation has become a motivating topic recently and will potentially open new avenues for the study of small animal imaging. Cerenkov-based optical imaging taken from living animals in vivo has been studied with two-dimensional (2D) planar geometry and three-dimensional (3D) homogeneous mouse model. In this study, we performed 3D Cerenkov-based luminescence tomography (CLT) using a heterogeneous mouse model with an implanted Na131I radioactive source, which provided the accurate location for the reconstructed source. Furthermore, single photon emission computed tomography (SPECT) was utilized to verify the results of 3D CLT. We reconstructed the localization and intensity of an embedded radioactive source with various concentrations, and established a quantitative relationship between the radiotracer activity and the reconstructed intensity. The results showed the ability of in vivo CLT to recover the radioactive probe distribution in the heterogeneous mouse model and the potential of a SPECT imaging validation strategy to verify the results of optical molecular tomography.

© 2010 OSA

OCIS Codes
(110.6960) Imaging systems : Tomography
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(260.3800) Physical optics : Luminescence
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: July 6, 2010
Revised Manuscript: September 8, 2010
Manuscript Accepted: November 2, 2010
Published: November 9, 2010

Zhenhua Hu, Jimin Liang, Weidong Yang, Weiwei Fan, Congye Li, Xiaowei Ma, Xueli Chen, Xiaopeng Ma, Xiangsi Li, Xiaochao Qu, Jing Wang, Feng Cao, and Jie Tian, "Experimental Cerenkov luminescence tomography of the mouse model with SPECT imaging validation," Opt. Express 18, 24441-24450 (2010)

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