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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 24 — Nov. 22, 2010
  • pp: 24983–24993

Quantitative analysis of collagen fiber organization in injured tendons using Fourier transform-second harmonic generation imaging

Mayandi Sivaguru, Sushmitha Durgam, Raghu Ambekar, David Luedtke, Glenn Fried, Allison Stewart, and Kimani C. Toussaint, Jr.  »View Author Affiliations

Optics Express, Vol. 18, Issue 24, pp. 24983-24993 (2010)

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Fourier transform-second harmonic generation (FT-SHG) imaging is used as a technique for evaluating collagenase-induced injury in horse tendons. The differences in collagen fiber organization between normal and injured tendon are quantified. Results indicate that the organization of collagen fibers is regularly oriented in normal tendons and randomly organized in injured tendons. This is further supported through the use of additional metrics, in particular, the number of dark (no/minimal signal) and isotropic (no preferred fiber orientation) regions in the images, and the ratio of forward-to-backward second-harmonic intensity. FT-SHG microscopy is also compared with the conventional polarized light microscopy and is shown to be more sensitive to assessing injured tendons than the latter. Moreover, sample preparation artifacts that affect the quantitative evaluation of collagen fiber organization can be circumvented by using FT-SHG microscopy. The technique has potential as an assessment tool for evaluating the impact of various injuries that affect collagen fiber organization.

© 2010 OSA

OCIS Codes
(100.2960) Image processing : Image analysis
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 20, 2010
Revised Manuscript: October 15, 2010
Manuscript Accepted: November 8, 2010
Published: November 15, 2010

Mayandi Sivaguru, Sushmitha Durgam, Raghu Ambekar, David Luedtke, Glenn Fried, Allison Stewart, and Kimani C. Toussaint, "Quantitative analysis of collagen fiber organization in injured tendons using Fourier transform-second harmonic generation imaging," Opt. Express 18, 24983-24993 (2010)

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