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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 25 — Dec. 6, 2010
  • pp: 25519–25534

Spectroscopic optical coherence elastography

Steven G. Adie, Xing Liang, Brendan F. Kennedy, Renu John, David D. Sampson, and Stephen A. Boppart  »View Author Affiliations

Optics Express, Vol. 18, Issue 25, pp. 25519-25534 (2010)

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We present an optical technique to image the frequency-dependent complex mechanical response of a viscoelastic sample. Three-dimensional hyperspectral data, comprising two-dimensional B-mode images and a third dimension corresponding to vibration frequency, were acquired from samples undergoing external mechanical excitation in the audio-frequency range. We describe the optical coherence tomography (OCT) signal when vibration is applied to a sample and detail the processing and acquisition techniques used to extract the local complex mechanical response from three-dimensional data that, due to a wide range of vibration frequencies, possess a wide range of sample velocities. We demonstrate frequency-dependent contrast of the displacement amplitude and phase of a silicone phantom containing inclusions of higher stiffness. Measurements of an ex vivo tumor margin demonstrate distinct spectra between adipose and tumor regions, and images of displacement amplitude and phase demonstrated spatially-resolved contrast. Contrast was also observed in displacement amplitude and phase images of a rat muscle sample. These results represent the first demonstration of mechanical spectroscopy based on B-mode OCT imaging. Spectroscopic optical coherence elastography (S-OCE) provides a high-resolution imaging capability for the detection of tissue pathologies that are characterized by a frequency-dependent viscoelastic response.

© 2010 OSA

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(350.0350) Other areas of optics : Other areas of optics
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Imaging Systems

Original Manuscript: August 24, 2010
Revised Manuscript: October 28, 2010
Manuscript Accepted: October 29, 2010
Published: November 22, 2010

Steven G. Adie, Xing Liang, Brendan F. Kennedy, Renu John, David D. Sampson, and Stephen A. Boppart, "Spectroscopic optical coherence elastography," Opt. Express 18, 25519-25534 (2010)

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