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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 25 — Dec. 6, 2010
  • pp: 25794–25807

A robust collagen scoring method for human liver fibrosis by second harmonic microscopy

Thomas Guilbert, Christophe Odin, Yann Le Grand, Luc Gailhouste, Bruno Turlin, Frédéric Ezan, Yoann Désille, Georges Baffet, and Dominique Guyader  »View Author Affiliations

Optics Express, Vol. 18, Issue 25, pp. 25794-25807 (2010)

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Second Harmonic Generation (SHG) microscopy offers the opportunity to image collagen of type I without staining. We recently showed that a simple scoring method, based on SHG images of histological human liver biopsies, correlates well with the Metavir assessment of fibrosis level (Gailhouste et al., J. Hepatol., 2010). In this article, we present a detailed study of this new scoring method with two different objective lenses. By using measurements of the objectives point spread functions and of the photomultiplier gain, and a simple model of the SHG intensity, we show that our scoring method, applied to human liver biopsies, is robust to the objective’s numerical aperture (NA) for low NA, the choice of the reference sample and laser power, and the spatial sampling rate. The simplicity and robustness of our collagen scoring method may open new opportunities in the quantification of collagen content in different organs, which is of main importance in providing diagnostic information and evaluation of therapeutic efficiency.

© 2010 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 2, 2010
Revised Manuscript: October 18, 2010
Manuscript Accepted: October 21, 2010
Published: November 24, 2010

Thomas Guilbert, Christophe Odin, Yann Le Grand, Luc Gailhouste, Bruno Turlin, Frédérick Ezan, Yoann Désille, Georges Baffet, and Dominique Guyader, "A robust collagen scoring method for human liver fibrosis by second harmonic microscopy," Opt. Express 18, 25794-25807 (2010)

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