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A robust collagen scoring method for human liver fibrosis by second harmonic microscopyThomas Guilbert, Christophe Odin, Yann Le Grand, Luc Gailhouste, Bruno Turlin, Frédérick Ezan, Yoann Désille, Georges Baffet, and Dominique Guyader »View Author Affiliations
Thomas Guilbert,1
Christophe Odin,1,*
Yann Le Grand,1,2
Luc Gailhouste,3
Bruno Turlin,4
Frédéric Ezan,3
Yoann Désille,5
Georges Baffet,3
and Dominique Guyader5
1Institut of Physics of Rennes IPR/UMR CNRS 6251, University of Rennes I, Campus de Beaulieu, Bat 11A, 35042 Rennes Cedex, France 2Laboratoire de Spectrométrie et Optique Laser, EA 938, University of Bretagne Occidentale, 29238 Brest, France 3EA eRAIC 4427, IFR 140, University of Rennes 1, 35033 Rennes, France 4Department of Pathology, Hôpital Pontchaillou, 35033 Rennes, France 5Liver Department Hôpital Pontchaillou, 35033 Rennes, France *Corresponding author: christophe.odin@univ-rennes1.fr |
Optics Express, Vol. 18, Issue 25, pp. 25794-25807 (2010)
http://dx.doi.org/10.1364/OE.18.025794
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Abstract
Second Harmonic Generation (SHG) microscopy offers the opportunity to image collagen of type I without staining. We recently showed that a simple scoring method, based on SHG images of histological human liver biopsies, correlates well with the Metavir assessment of fibrosis level (Gailhouste et al., J. Hepatol., 2010). In this article, we present a detailed study of this new scoring method with two different objective lenses. By using measurements of the objectives point spread functions and of the photomultiplier gain, and a simple model of the SHG intensity, we show that our scoring method, applied to human liver biopsies, is robust to the objective’s numerical aperture (NA) for low NA, the choice of the reference sample and laser power, and the spatial sampling rate. The simplicity and robustness of our collagen scoring method may open new opportunities in the quantification of collagen content in different organs, which is of main importance in providing diagnostic information and evaluation of therapeutic efficiency.
© 2010 Optical Society of America
OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(180.4315) Microscopy : Nonlinear microscopy
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: September 2, 2010
Revised Manuscript: October 18, 2010
Manuscript Accepted: October 21, 2010
Published: November 24, 2010
Citation
Thomas Guilbert, Christophe Odin, Yann Le Grand, Luc Gailhouste, Bruno Turlin, Frédérick Ezan, Yoann Désille, Georges Baffet, and Dominique Guyader, "A robust collagen scoring method for human liver fibrosis by second harmonic
microscopy," Opt. Express 18, 25794-25807 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-25-25794
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References
- S. L. Friedman, “Liver fibrosis - from bench to bedside,” J. Hepatol. 38, 38–53 (2003). [CrossRef]
- R. Bataller, and D. A. Brenner, “Liver fibrosis,” J. Clin. Invest. 115, 209–218 (2005). [PubMed]
- N. H. Afdhal, and D. Nunes, “Evaluation of liver fibrosis: a concise review,” Am. J. Gastroenterol. 99, 1160–1174 (2004). [CrossRef] [PubMed]
- G. L. Davis, J. E. Albright, S. F. Cook, and D. M. Rosenberg, “Projecting future complications of chronic hepatitis C in the United States,” Liver Transpl. 9, 331–338 (2003). [CrossRef] [PubMed]
- F. Oberti, E. Valsesia, C. Pilette, M. C. Rousselet, P. Bedossa, C. Aub, Y. Gallois, H. Rifflet, M. Y. Maga, D. Penneau-Fontbonne, and P. Cals, “Noninvasive diagnosis of hepatic fibrosis or cirrhosis,” Gastroenterology 113, 1609–1616 (1997). [CrossRef] [PubMed]
- N. H. Afdhal, A. P. Keaveny, S. B. Cohen, D. P. Nunes, N. Maldonado, M. O’Brien, and P. J. Stone, “Urinary assays for desmosine and hydroxylysylpyridinoline in the detection of cirrhosis,” J. Hepatol. 27, 993–1002 (1997). [CrossRef]
- S. C. Faria, K. Ganesan, I. Mwangi, M. Shiehmorteza, B. Viamonte, S. Mazhar, M. Peterson, Y. Kono, C. Santillan, G. Casola, and C. B. Sirlin, “MR Imaging of Liver Fibrosis: Current State of the Art,” Radiographics 29, 1615–1635 (2009). [CrossRef] [PubMed]
- C. Aube, F. Oberti, N. Koralin, M.-A. Namour, D. Loisel, M. C. Rousselet, P. Bedossa, H. Rifflet, M. Y. Maga, C. Penneau, C. Caron, and P. Cals, “Ultrasonographic diagnosis of hepatic fibrosis or cirrhosis,” J. Hepatol. 30, 472–478 (1999). [CrossRef] [PubMed]
- H. A. Saleh, and A. H. Abu-Rashed, “Liver Biopsy Remains the Gold Standard for Evaluation of Chronic Hepatitis and Fibrosis,” J. Gastrointestin. Liver Dis. 16, 425–426 (2007).
- P. Bedossa, D. Dargre, and V. Paradis, “Sampling variability of liver fibrosis in chronic hepatitis C,” J. Hepatol. 38, 1356–1358 (2003).
- P. J. Scheuer, “Classification of of chronic viral hepatitis: a need for reassessment,” J. Hepatol. 13, 372–374 (1991). [CrossRef] [PubMed]
- K. Ishak, A. Baptista, L. Bianchi, F. Callea, J. De Groote, F. Gudat, H. Denk, V. Desmet, G. Korb, and R. N. MacSween, “Histological grading and staging of chronic hepatitis,” J. Hepatol. 22, 696–699 (1995). [CrossRef] [PubMed]
- The French METAVIR Cooperative Study Group, “Intraobserver, interobserver variations in liver biopsy interpretation in patients with chronic hepatitis C,” J. Hepatol. 20, 15–20 (1995).
- M. Masseroli, and T. Caballero, “F. OValle, R. M. Del Moral, A. Perez-Milena, and R. G. Del Moral “Automatic quantification of liver fibrosis design and validation of a new image analysis method: comparison with semiquantitative indexes of fibrosis,”,” J. Hepatol. 32, 453–464 (2000). [CrossRef] [PubMed]
- A. Y. Hui, C.-T. Liew, M. Y.-Y. Go, A. M.-L. Chim, H. L.-Y. Chan, N. W.-Y. Leung, and J. J.-Y. Sung, “Quantitative assessment of fibrosis in liver biopsies from patients with chronic hepatitis B,” Liver Int. 24, 611–618 (2004). [CrossRef]
- M. A. Friedenberg, L. Miller, C. Y. Chung, F. Fleszler, F. L. Banson, R. Thomas, K. P. Swartz, and F. K. Friedenberg, “Simplified method of hepatic fibrosis quantification: design of a new morphometric analysis application,” Liver Int. 25, 1156–1161 (2005). [CrossRef]
- K. König, “Multiphoton microscopy in life sciences,” J. Microsc. 200, 83–104 (2000). [CrossRef] [PubMed]
- A. Zoumi, A. Yeh, and B. J. Tromberg, “Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence,” Proc. Natl. Acad. Sci. U.S.A.. 20, 11014–11019 (2002). [CrossRef]
- P. J. Campagnola, and L. M. Loew, “Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms,” Nat. Biotechnol. 21, 1356–1360 (2003). [CrossRef]
- W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21, 1369–1377 (2003). [CrossRef]
- W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, “Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation,” Proc. Natl. Acad. Sci. U.S.A. 100, 7075–7080 (2003). [CrossRef] [PubMed]
- S. Roth, and I. Freund, “Second harmonic generation in collagen,” J. Chem. Phys. 70, 1637–1643 (1979). [CrossRef]
- S. W. Chu, S. Y. Chen, G. W. Chern, T. H. Tsai, Y. C. Chen, B. L. Lin, and C. K. Sun, “Studies of χ(2)/χ(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy,” Biophys. J. 86, 3914–3922 (2004). [CrossRef] [PubMed]
- R. M. Williams, W. R. Zipfel, and W. W. Webb, “Interpreting Second-Harmonic Generation Images of Collagen I Fibrils,” Biophys. J. 88, 1377–1386 (2005). [CrossRef]
- S. V. Plotnikov, A. C. Millard, and P. J. Campagnola, and W. A. Mohler, “Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres,” Biophys. J. 90, 693–703 (2006). [CrossRef]
- C. Odin, Y. Le Grand, A. Renault, L. Gailhouste, and G. Baffet, “Orientation fields of nonlinear biological fibrils by second harmonic generation microscopy,” J. Microsc. 229, 32–38 (2008). [CrossRef]
- C. Odin, T. Guilbert, A. Alkilani, O. Boryskina, V. Fleury, and Y. Le Grand, “Collagen and myosin characterization by orientation field second harmonic microscopy,” Opt. Express 16, 16151–16165 (2008). [CrossRef] [PubMed]
- C. Odin, C. Heichette, D. Chretien, and Y. Le Grand, “Second harmonic microscopy of axonemes,” Opt. Express 17, 9235–9240 (2009). [CrossRef] [PubMed]
- S. Psilodimitrakopoulos, D. Artigas, G. Soria, I. Amat-Roldan, A. Planas, and P. Loza-Alvarez, “Quantitative discrimination between endogenousSHG sources in mammalian tissue, based ontheir polarization response,” Opt. Express 17, 10168–10176 (2009). [CrossRef] [PubMed]
- S. Psilodimitrakopoulos, V. Petegnief, G. Soria, I. Amat-Roldan, D. Artigas, A. Planas, and P. Loza-Alvarez, “Estimation of the effective orientation of the SHG source in primary cortical neurons,” Opt. Express 17, 14418–14425 (2009). [CrossRef] [PubMed]
- M. Strupler, A. Pena, M. Hernest, P. Tharaux, J. Martin, E. Beaurepaire, and M. Schanne-Klein, “Second harmonic imaging and scoring of collagen in fibrotic tissues,” Opt. Express 15, 4054–4065 (2007). [CrossRef] [PubMed]
- A.-M. Pena, A. Fabre, D. Dbarre, J. Marchal-Somme, B. Crestani, J.-L. Martin, E. Beaurepaire, and M.-C. Schanne-Klein, “Three-dimensional investigation and scoring of extracellular matrix remodeling during lung fibrosis using multiphoton microscopy,” Microsc. Res. Tech. 70, 162–170 (2007). [CrossRef]
- X. Han, R. M. Burke, M. L. Zettel, P. Tang, and E. B. Brown, “Second harmonic properties of tumor collagen: determining the structural relationship between reactive stroma and healthy stroma,” Opt. Express 16, 1846–1859 (2008), http://www.opticsinfobase.org/abstract.cfm?URI=oe-16-3-1846. [CrossRef] [PubMed]
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
- L. Gailhouste, Y. Le Grand, C. Odin, D. Guyader, B. Turlin, F. Ezan, Y. Dsille, T. Guilbert, A. Bessard, C. Frmin, N. Theret, and G. Baffet, “Fibrillar Collagen Scoring by Second Harmonic Microscopy as a New Tool in the Assessment of Liver Fibrosis,” J. Hepatol. 52, 398–406 (2010). [CrossRef]
- P. Bedossa, “Harmony in liver fibrosis.,” J. Hepatol. 52, 313–314 (2010). [CrossRef]
- J. Mertz, and L. Moreaux, “Second-harmonic generation by focused excitation of inhomogeneously distributed scatterers,” Opt. Commun. 196, 325–330 (2001). [CrossRef]
- L. I.-K. Lin, “A concordance correlation coefficient to evaluate reproducibility,” Biometrics 45, 255–268 (1989). [CrossRef]
- L. I.-K. Lin, “A note on the concordance correlation coefficient,” Biometrics 56, 324–325 (2000).
- H. Bao, A. Boussioutas, R. Jeremy, S. Russell, and M. Gu, “Second harmonic generation imaging via nonlinear endomicroscopy,” Opt. Express 18, 1255–1260 (2010). [CrossRef] [PubMed]
Abu-Rashed, A. H.
Afdhal, N. H.
Albright, J. E.
Alkilani, A.
Amat-Roldan, I.
Artigas, D.
Aub, C.
Aube, C.
Baffet, G.
Banson, F. L.
Bao, H.
Baptista, A.
Bataller, R.
Beaurepaire, E.
Bedossa, P.
Bessard, A.
Bianchi, L.
Boryskina, O.
Boussioutas, A.
Brenner, D. A.
Brown, E. B.
Burke, R. M.
Caballero, T.
Callea, F.
Cals, P.
Campagnola, P. J.
Caron, C.
Casola, G.
Chan, H. L.-Y.
Chang, S.
Chen, S. Y.
Chen, Y. C.
Cheng, C. L.
Chern, G. W.
Chia, S. M.
Chim, A. M.-L.
Chretien, D.
Christie, R.
Chu, S. W.
Chung, C. Y.
Cohen, S. B.
Cook, S. F.
Crestani, B.
Dargre, D.
Davis, G. L.
Dbarre, D.
De Groote, J.
Denk, H.
Desmet, V.
Dsille, Y.
Ezan, F.
Fabre, A.
Faria, S. C.
Fleszler, F.
Fleury, V.
Freund, I.
Friedenberg, F. K.
Friedenberg, M. A.
Friedman, S. L.
Frmin, C.
Gailhouste, L.
Gallois, Y.
Ganesan, K.
Go, M. Y.-Y.
Gu, M.
Gudat, F.
Guilbert, T.
Guyader, D.
Han, X.
Heichette, C.
Hernest, M.
Hui, A. Y.
Hyman, B. T.
Ishak, K.
Jeremy, R.
Kang, C. H.
Keaveny, A. P.
König, K.
Kono, Y.
Koralin, N.
Korb, G.
Le Grand, Y.
Leung, N. W.-Y.
Li Wei, C.
Liew, C.-T.
Lin, B. L.
Lin, L. I.-K.
Loew, L. M.
Loisel, D.
Loza-Alvarez, P.
MacSween, R. N.
Maga, M. Y.
Maldonado, N.
Marchal-Somme, J.
Martin, J.
Martin, J.-L.
Masseroli, M.
Mazhar, S.
Mertz, J.
Millard, A. C.
Miller, L.
Mohler, W. A.
Moreaux, L.
Mwangi, I.
Namour, M.-A.
Nikitin, A. Y.
Nunes, D.
Nunes, D. P.
O’Brien, M.
Oberti, F.
Odin, C.
Paradis, V.
Pena, A.
Pena, A.-M.
Penneau, C.
Penneau-Fontbonne, D.
Petegnief, V.
Peterson, M.
Pilette, C.
Planas, A.
Plotnikov, S. V.
Psilodimitrakopoulos, S.
Raja, A. M.
Rajapakse, J. C.
Renault, A.
Rifflet, H.
Rosenberg, D. M.
Roth, S.
Rousselet, M. C.
Russell, S.
Saleh, H. A.
Santillan, C.
Schanne-Klein, M.
Schanne-Klein, M.-C.
Scheuer, P. J.
Shiehmorteza, M.
Shing Chen, C.
Sirlin, C. B.
So, P. T. C.
Soria, G.
Stone, P. J.
Strupler, M.
Sun, C. K.
Sung, J. J.-Y.
Swartz, K. P.
Tai, D. C. S.
Tan, N.
Tang, H.-H.
Tang, P.
Tharaux, P.
Theret, N.
Thomas, R.
Tromberg, B. J.
Tsai, T. H.
Turlin, B.
Valsesia, E.
Viamonte, B.
Webb, W. W.
Wee, A.
Williams, R. M.
Xiao, G.
Xu, S.
Yeh, A.
Yu, H.
Zettel, M. L.
Zipfel, W. R.
Zoumi, A.
- H. A. Saleh, and A. H. Abu-Rashed, “Liver Biopsy Remains the Gold Standard for Evaluation of Chronic Hepatitis and Fibrosis,” J. Gastrointestin. Liver Dis. 16, 425–426 (2007).
- N. H. Afdhal, and D. Nunes, “Evaluation of liver fibrosis: a concise review,” Am. J. Gastroenterol. 99, 1160–1174 (2004). [CrossRef] [PubMed]
- N. H. Afdhal, A. P. Keaveny, S. B. Cohen, D. P. Nunes, N. Maldonado, M. O’Brien, and P. J. Stone, “Urinary assays for desmosine and hydroxylysylpyridinoline in the detection of cirrhosis,” J. Hepatol. 27, 993–1002 (1997). [CrossRef]
- G. L. Davis, J. E. Albright, S. F. Cook, and D. M. Rosenberg, “Projecting future complications of chronic hepatitis C in the United States,” Liver Transpl. 9, 331–338 (2003). [CrossRef] [PubMed]
- S. Psilodimitrakopoulos, D. Artigas, G. Soria, I. Amat-Roldan, A. Planas, and P. Loza-Alvarez, “Quantitative discrimination between endogenousSHG sources in mammalian tissue, based ontheir polarization response,” Opt. Express 17, 10168–10176 (2009). [CrossRef] [PubMed]
- S. Psilodimitrakopoulos, V. Petegnief, G. Soria, I. Amat-Roldan, D. Artigas, A. Planas, and P. Loza-Alvarez, “Estimation of the effective orientation of the SHG source in primary cortical neurons,” Opt. Express 17, 14418–14425 (2009). [CrossRef] [PubMed]
- S. Psilodimitrakopoulos, V. Petegnief, G. Soria, I. Amat-Roldan, D. Artigas, A. Planas, and P. Loza-Alvarez, “Estimation of the effective orientation of the SHG source in primary cortical neurons,” Opt. Express 17, 14418–14425 (2009). [CrossRef] [PubMed]
- S. Psilodimitrakopoulos, D. Artigas, G. Soria, I. Amat-Roldan, A. Planas, and P. Loza-Alvarez, “Quantitative discrimination between endogenousSHG sources in mammalian tissue, based ontheir polarization response,” Opt. Express 17, 10168–10176 (2009). [CrossRef] [PubMed]
- F. Oberti, E. Valsesia, C. Pilette, M. C. Rousselet, P. Bedossa, C. Aub, Y. Gallois, H. Rifflet, M. Y. Maga, D. Penneau-Fontbonne, and P. Cals, “Noninvasive diagnosis of hepatic fibrosis or cirrhosis,” Gastroenterology 113, 1609–1616 (1997). [CrossRef] [PubMed]
- C. Aube, F. Oberti, N. Koralin, M.-A. Namour, D. Loisel, M. C. Rousselet, P. Bedossa, H. Rifflet, M. Y. Maga, C. Penneau, C. Caron, and P. Cals, “Ultrasonographic diagnosis of hepatic fibrosis or cirrhosis,” J. Hepatol. 30, 472–478 (1999). [CrossRef] [PubMed]
- L. Gailhouste, Y. Le Grand, C. Odin, D. Guyader, B. Turlin, F. Ezan, Y. Dsille, T. Guilbert, A. Bessard, C. Frmin, N. Theret, and G. Baffet, “Fibrillar Collagen Scoring by Second Harmonic Microscopy as a New Tool in the Assessment of Liver Fibrosis,” J. Hepatol. 52, 398–406 (2010). [CrossRef]
- C. Odin, Y. Le Grand, A. Renault, L. Gailhouste, and G. Baffet, “Orientation fields of nonlinear biological fibrils by second harmonic generation microscopy,” J. Microsc. 229, 32–38 (2008). [CrossRef]
- M. A. Friedenberg, L. Miller, C. Y. Chung, F. Fleszler, F. L. Banson, R. Thomas, K. P. Swartz, and F. K. Friedenberg, “Simplified method of hepatic fibrosis quantification: design of a new morphometric analysis application,” Liver Int. 25, 1156–1161 (2005). [CrossRef]
- K. Ishak, A. Baptista, L. Bianchi, F. Callea, J. De Groote, F. Gudat, H. Denk, V. Desmet, G. Korb, and R. N. MacSween, “Histological grading and staging of chronic hepatitis,” J. Hepatol. 22, 696–699 (1995). [CrossRef] [PubMed]
- R. Bataller, and D. A. Brenner, “Liver fibrosis,” J. Clin. Invest. 115, 209–218 (2005). [PubMed]
- M. Strupler, A. Pena, M. Hernest, P. Tharaux, J. Martin, E. Beaurepaire, and M. Schanne-Klein, “Second harmonic imaging and scoring of collagen in fibrotic tissues,” Opt. Express 15, 4054–4065 (2007). [CrossRef] [PubMed]
- A.-M. Pena, A. Fabre, D. Dbarre, J. Marchal-Somme, B. Crestani, J.-L. Martin, E. Beaurepaire, and M.-C. Schanne-Klein, “Three-dimensional investigation and scoring of extracellular matrix remodeling during lung fibrosis using multiphoton microscopy,” Microsc. Res. Tech. 70, 162–170 (2007). [CrossRef]
- P. Bedossa, “Harmony in liver fibrosis.,” J. Hepatol. 52, 313–314 (2010). [CrossRef]
- P. Bedossa, D. Dargre, and V. Paradis, “Sampling variability of liver fibrosis in chronic hepatitis C,” J. Hepatol. 38, 1356–1358 (2003).
- C. Aube, F. Oberti, N. Koralin, M.-A. Namour, D. Loisel, M. C. Rousselet, P. Bedossa, H. Rifflet, M. Y. Maga, C. Penneau, C. Caron, and P. Cals, “Ultrasonographic diagnosis of hepatic fibrosis or cirrhosis,” J. Hepatol. 30, 472–478 (1999). [CrossRef] [PubMed]
- F. Oberti, E. Valsesia, C. Pilette, M. C. Rousselet, P. Bedossa, C. Aub, Y. Gallois, H. Rifflet, M. Y. Maga, D. Penneau-Fontbonne, and P. Cals, “Noninvasive diagnosis of hepatic fibrosis or cirrhosis,” Gastroenterology 113, 1609–1616 (1997). [CrossRef] [PubMed]
- L. Gailhouste, Y. Le Grand, C. Odin, D. Guyader, B. Turlin, F. Ezan, Y. Dsille, T. Guilbert, A. Bessard, C. Frmin, N. Theret, and G. Baffet, “Fibrillar Collagen Scoring by Second Harmonic Microscopy as a New Tool in the Assessment of Liver Fibrosis,” J. Hepatol. 52, 398–406 (2010). [CrossRef]
- K. Ishak, A. Baptista, L. Bianchi, F. Callea, J. De Groote, F. Gudat, H. Denk, V. Desmet, G. Korb, and R. N. MacSween, “Histological grading and staging of chronic hepatitis,” J. Hepatol. 22, 696–699 (1995). [CrossRef] [PubMed]
- R. Bataller, and D. A. Brenner, “Liver fibrosis,” J. Clin. Invest. 115, 209–218 (2005). [PubMed]
- X. Han, R. M. Burke, M. L. Zettel, P. Tang, and E. B. Brown, “Second harmonic properties of tumor collagen: determining the structural relationship between reactive stroma and healthy stroma,” Opt. Express 16, 1846–1859 (2008), http://www.opticsinfobase.org/abstract.cfm?URI=oe-16-3-1846. [CrossRef] [PubMed]
- X. Han, R. M. Burke, M. L. Zettel, P. Tang, and E. B. Brown, “Second harmonic properties of tumor collagen: determining the structural relationship between reactive stroma and healthy stroma,” Opt. Express 16, 1846–1859 (2008), http://www.opticsinfobase.org/abstract.cfm?URI=oe-16-3-1846. [CrossRef] [PubMed]
- M. Masseroli, and T. Caballero, “F. OValle, R. M. Del Moral, A. Perez-Milena, and R. G. Del Moral “Automatic quantification of liver fibrosis design and validation of a new image analysis method: comparison with semiquantitative indexes of fibrosis,”,” J. Hepatol. 32, 453–464 (2000). [CrossRef] [PubMed]
- K. Ishak, A. Baptista, L. Bianchi, F. Callea, J. De Groote, F. Gudat, H. Denk, V. Desmet, G. Korb, and R. N. MacSween, “Histological grading and staging of chronic hepatitis,” J. Hepatol. 22, 696–699 (1995). [CrossRef] [PubMed]
- C. Aube, F. Oberti, N. Koralin, M.-A. Namour, D. Loisel, M. C. Rousselet, P. Bedossa, H. Rifflet, M. Y. Maga, C. Penneau, C. Caron, and P. Cals, “Ultrasonographic diagnosis of hepatic fibrosis or cirrhosis,” J. Hepatol. 30, 472–478 (1999). [CrossRef] [PubMed]
- F. Oberti, E. Valsesia, C. Pilette, M. C. Rousselet, P. Bedossa, C. Aub, Y. Gallois, H. Rifflet, M. Y. Maga, D. Penneau-Fontbonne, and P. Cals, “Noninvasive diagnosis of hepatic fibrosis or cirrhosis,” Gastroenterology 113, 1609–1616 (1997). [CrossRef] [PubMed]
- S. V. Plotnikov, A. C. Millard, and P. J. Campagnola, and W. A. Mohler, “Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres,” Biophys. J. 90, 693–703 (2006). [CrossRef]
- P. J. Campagnola, and L. M. Loew, “Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms,” Nat. Biotechnol. 21, 1356–1360 (2003). [CrossRef]
- C. Aube, F. Oberti, N. Koralin, M.-A. Namour, D. Loisel, M. C. Rousselet, P. Bedossa, H. Rifflet, M. Y. Maga, C. Penneau, C. Caron, and P. Cals, “Ultrasonographic diagnosis of hepatic fibrosis or cirrhosis,” J. Hepatol. 30, 472–478 (1999). [CrossRef] [PubMed]
- S. C. Faria, K. Ganesan, I. Mwangi, M. Shiehmorteza, B. Viamonte, S. Mazhar, M. Peterson, Y. Kono, C. Santillan, G. Casola, and C. B. Sirlin, “MR Imaging of Liver Fibrosis: Current State of the Art,” Radiographics 29, 1615–1635 (2009). [CrossRef] [PubMed]
- A. Y. Hui, C.-T. Liew, M. Y.-Y. Go, A. M.-L. Chim, H. L.-Y. Chan, N. W.-Y. Leung, and J. J.-Y. Sung, “Quantitative assessment of fibrosis in liver biopsies from patients with chronic hepatitis B,” Liver Int. 24, 611–618 (2004). [CrossRef]
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
- S. W. Chu, S. Y. Chen, G. W. Chern, T. H. Tsai, Y. C. Chen, B. L. Lin, and C. K. Sun, “Studies of χ(2)/χ(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy,” Biophys. J. 86, 3914–3922 (2004). [CrossRef] [PubMed]
- S. W. Chu, S. Y. Chen, G. W. Chern, T. H. Tsai, Y. C. Chen, B. L. Lin, and C. K. Sun, “Studies of χ(2)/χ(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy,” Biophys. J. 86, 3914–3922 (2004). [CrossRef] [PubMed]
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
- S. W. Chu, S. Y. Chen, G. W. Chern, T. H. Tsai, Y. C. Chen, B. L. Lin, and C. K. Sun, “Studies of χ(2)/χ(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy,” Biophys. J. 86, 3914–3922 (2004). [CrossRef] [PubMed]
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
- A. Y. Hui, C.-T. Liew, M. Y.-Y. Go, A. M.-L. Chim, H. L.-Y. Chan, N. W.-Y. Leung, and J. J.-Y. Sung, “Quantitative assessment of fibrosis in liver biopsies from patients with chronic hepatitis B,” Liver Int. 24, 611–618 (2004). [CrossRef]
- W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, “Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation,” Proc. Natl. Acad. Sci. U.S.A. 100, 7075–7080 (2003). [CrossRef] [PubMed]
- S. W. Chu, S. Y. Chen, G. W. Chern, T. H. Tsai, Y. C. Chen, B. L. Lin, and C. K. Sun, “Studies of χ(2)/χ(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy,” Biophys. J. 86, 3914–3922 (2004). [CrossRef] [PubMed]
- M. A. Friedenberg, L. Miller, C. Y. Chung, F. Fleszler, F. L. Banson, R. Thomas, K. P. Swartz, and F. K. Friedenberg, “Simplified method of hepatic fibrosis quantification: design of a new morphometric analysis application,” Liver Int. 25, 1156–1161 (2005). [CrossRef]
- N. H. Afdhal, A. P. Keaveny, S. B. Cohen, D. P. Nunes, N. Maldonado, M. O’Brien, and P. J. Stone, “Urinary assays for desmosine and hydroxylysylpyridinoline in the detection of cirrhosis,” J. Hepatol. 27, 993–1002 (1997). [CrossRef]
- G. L. Davis, J. E. Albright, S. F. Cook, and D. M. Rosenberg, “Projecting future complications of chronic hepatitis C in the United States,” Liver Transpl. 9, 331–338 (2003). [CrossRef] [PubMed]
- A.-M. Pena, A. Fabre, D. Dbarre, J. Marchal-Somme, B. Crestani, J.-L. Martin, E. Beaurepaire, and M.-C. Schanne-Klein, “Three-dimensional investigation and scoring of extracellular matrix remodeling during lung fibrosis using multiphoton microscopy,” Microsc. Res. Tech. 70, 162–170 (2007). [CrossRef]
- P. Bedossa, D. Dargre, and V. Paradis, “Sampling variability of liver fibrosis in chronic hepatitis C,” J. Hepatol. 38, 1356–1358 (2003).
- G. L. Davis, J. E. Albright, S. F. Cook, and D. M. Rosenberg, “Projecting future complications of chronic hepatitis C in the United States,” Liver Transpl. 9, 331–338 (2003). [CrossRef] [PubMed]
- A.-M. Pena, A. Fabre, D. Dbarre, J. Marchal-Somme, B. Crestani, J.-L. Martin, E. Beaurepaire, and M.-C. Schanne-Klein, “Three-dimensional investigation and scoring of extracellular matrix remodeling during lung fibrosis using multiphoton microscopy,” Microsc. Res. Tech. 70, 162–170 (2007). [CrossRef]
- K. Ishak, A. Baptista, L. Bianchi, F. Callea, J. De Groote, F. Gudat, H. Denk, V. Desmet, G. Korb, and R. N. MacSween, “Histological grading and staging of chronic hepatitis,” J. Hepatol. 22, 696–699 (1995). [CrossRef] [PubMed]
- K. Ishak, A. Baptista, L. Bianchi, F. Callea, J. De Groote, F. Gudat, H. Denk, V. Desmet, G. Korb, and R. N. MacSween, “Histological grading and staging of chronic hepatitis,” J. Hepatol. 22, 696–699 (1995). [CrossRef] [PubMed]
- K. Ishak, A. Baptista, L. Bianchi, F. Callea, J. De Groote, F. Gudat, H. Denk, V. Desmet, G. Korb, and R. N. MacSween, “Histological grading and staging of chronic hepatitis,” J. Hepatol. 22, 696–699 (1995). [CrossRef] [PubMed]
- L. Gailhouste, Y. Le Grand, C. Odin, D. Guyader, B. Turlin, F. Ezan, Y. Dsille, T. Guilbert, A. Bessard, C. Frmin, N. Theret, and G. Baffet, “Fibrillar Collagen Scoring by Second Harmonic Microscopy as a New Tool in the Assessment of Liver Fibrosis,” J. Hepatol. 52, 398–406 (2010). [CrossRef]
- L. Gailhouste, Y. Le Grand, C. Odin, D. Guyader, B. Turlin, F. Ezan, Y. Dsille, T. Guilbert, A. Bessard, C. Frmin, N. Theret, and G. Baffet, “Fibrillar Collagen Scoring by Second Harmonic Microscopy as a New Tool in the Assessment of Liver Fibrosis,” J. Hepatol. 52, 398–406 (2010). [CrossRef]
- A.-M. Pena, A. Fabre, D. Dbarre, J. Marchal-Somme, B. Crestani, J.-L. Martin, E. Beaurepaire, and M.-C. Schanne-Klein, “Three-dimensional investigation and scoring of extracellular matrix remodeling during lung fibrosis using multiphoton microscopy,” Microsc. Res. Tech. 70, 162–170 (2007). [CrossRef]
- S. C. Faria, K. Ganesan, I. Mwangi, M. Shiehmorteza, B. Viamonte, S. Mazhar, M. Peterson, Y. Kono, C. Santillan, G. Casola, and C. B. Sirlin, “MR Imaging of Liver Fibrosis: Current State of the Art,” Radiographics 29, 1615–1635 (2009). [CrossRef] [PubMed]
- M. A. Friedenberg, L. Miller, C. Y. Chung, F. Fleszler, F. L. Banson, R. Thomas, K. P. Swartz, and F. K. Friedenberg, “Simplified method of hepatic fibrosis quantification: design of a new morphometric analysis application,” Liver Int. 25, 1156–1161 (2005). [CrossRef]
- S. Roth, and I. Freund, “Second harmonic generation in collagen,” J. Chem. Phys. 70, 1637–1643 (1979). [CrossRef]
- M. A. Friedenberg, L. Miller, C. Y. Chung, F. Fleszler, F. L. Banson, R. Thomas, K. P. Swartz, and F. K. Friedenberg, “Simplified method of hepatic fibrosis quantification: design of a new morphometric analysis application,” Liver Int. 25, 1156–1161 (2005). [CrossRef]
- M. A. Friedenberg, L. Miller, C. Y. Chung, F. Fleszler, F. L. Banson, R. Thomas, K. P. Swartz, and F. K. Friedenberg, “Simplified method of hepatic fibrosis quantification: design of a new morphometric analysis application,” Liver Int. 25, 1156–1161 (2005). [CrossRef]
- S. L. Friedman, “Liver fibrosis - from bench to bedside,” J. Hepatol. 38, 38–53 (2003). [CrossRef]
- L. Gailhouste, Y. Le Grand, C. Odin, D. Guyader, B. Turlin, F. Ezan, Y. Dsille, T. Guilbert, A. Bessard, C. Frmin, N. Theret, and G. Baffet, “Fibrillar Collagen Scoring by Second Harmonic Microscopy as a New Tool in the Assessment of Liver Fibrosis,” J. Hepatol. 52, 398–406 (2010). [CrossRef]
- L. Gailhouste, Y. Le Grand, C. Odin, D. Guyader, B. Turlin, F. Ezan, Y. Dsille, T. Guilbert, A. Bessard, C. Frmin, N. Theret, and G. Baffet, “Fibrillar Collagen Scoring by Second Harmonic Microscopy as a New Tool in the Assessment of Liver Fibrosis,” J. Hepatol. 52, 398–406 (2010). [CrossRef]
- C. Odin, Y. Le Grand, A. Renault, L. Gailhouste, and G. Baffet, “Orientation fields of nonlinear biological fibrils by second harmonic generation microscopy,” J. Microsc. 229, 32–38 (2008). [CrossRef]
- F. Oberti, E. Valsesia, C. Pilette, M. C. Rousselet, P. Bedossa, C. Aub, Y. Gallois, H. Rifflet, M. Y. Maga, D. Penneau-Fontbonne, and P. Cals, “Noninvasive diagnosis of hepatic fibrosis or cirrhosis,” Gastroenterology 113, 1609–1616 (1997). [CrossRef] [PubMed]
- S. C. Faria, K. Ganesan, I. Mwangi, M. Shiehmorteza, B. Viamonte, S. Mazhar, M. Peterson, Y. Kono, C. Santillan, G. Casola, and C. B. Sirlin, “MR Imaging of Liver Fibrosis: Current State of the Art,” Radiographics 29, 1615–1635 (2009). [CrossRef] [PubMed]
- A. Y. Hui, C.-T. Liew, M. Y.-Y. Go, A. M.-L. Chim, H. L.-Y. Chan, N. W.-Y. Leung, and J. J.-Y. Sung, “Quantitative assessment of fibrosis in liver biopsies from patients with chronic hepatitis B,” Liver Int. 24, 611–618 (2004). [CrossRef]
- K. Ishak, A. Baptista, L. Bianchi, F. Callea, J. De Groote, F. Gudat, H. Denk, V. Desmet, G. Korb, and R. N. MacSween, “Histological grading and staging of chronic hepatitis,” J. Hepatol. 22, 696–699 (1995). [CrossRef] [PubMed]
- L. Gailhouste, Y. Le Grand, C. Odin, D. Guyader, B. Turlin, F. Ezan, Y. Dsille, T. Guilbert, A. Bessard, C. Frmin, N. Theret, and G. Baffet, “Fibrillar Collagen Scoring by Second Harmonic Microscopy as a New Tool in the Assessment of Liver Fibrosis,” J. Hepatol. 52, 398–406 (2010). [CrossRef]
- C. Odin, T. Guilbert, A. Alkilani, O. Boryskina, V. Fleury, and Y. Le Grand, “Collagen and myosin characterization by orientation field second harmonic microscopy,” Opt. Express 16, 16151–16165 (2008). [CrossRef] [PubMed]
- L. Gailhouste, Y. Le Grand, C. Odin, D. Guyader, B. Turlin, F. Ezan, Y. Dsille, T. Guilbert, A. Bessard, C. Frmin, N. Theret, and G. Baffet, “Fibrillar Collagen Scoring by Second Harmonic Microscopy as a New Tool in the Assessment of Liver Fibrosis,” J. Hepatol. 52, 398–406 (2010). [CrossRef]
- X. Han, R. M. Burke, M. L. Zettel, P. Tang, and E. B. Brown, “Second harmonic properties of tumor collagen: determining the structural relationship between reactive stroma and healthy stroma,” Opt. Express 16, 1846–1859 (2008), http://www.opticsinfobase.org/abstract.cfm?URI=oe-16-3-1846. [CrossRef] [PubMed]
- A. Y. Hui, C.-T. Liew, M. Y.-Y. Go, A. M.-L. Chim, H. L.-Y. Chan, N. W.-Y. Leung, and J. J.-Y. Sung, “Quantitative assessment of fibrosis in liver biopsies from patients with chronic hepatitis B,” Liver Int. 24, 611–618 (2004). [CrossRef]
- W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, “Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation,” Proc. Natl. Acad. Sci. U.S.A. 100, 7075–7080 (2003). [CrossRef] [PubMed]
- K. Ishak, A. Baptista, L. Bianchi, F. Callea, J. De Groote, F. Gudat, H. Denk, V. Desmet, G. Korb, and R. N. MacSween, “Histological grading and staging of chronic hepatitis,” J. Hepatol. 22, 696–699 (1995). [CrossRef] [PubMed]
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
- N. H. Afdhal, A. P. Keaveny, S. B. Cohen, D. P. Nunes, N. Maldonado, M. O’Brien, and P. J. Stone, “Urinary assays for desmosine and hydroxylysylpyridinoline in the detection of cirrhosis,” J. Hepatol. 27, 993–1002 (1997). [CrossRef]
- K. König, “Multiphoton microscopy in life sciences,” J. Microsc. 200, 83–104 (2000). [CrossRef] [PubMed]
- S. C. Faria, K. Ganesan, I. Mwangi, M. Shiehmorteza, B. Viamonte, S. Mazhar, M. Peterson, Y. Kono, C. Santillan, G. Casola, and C. B. Sirlin, “MR Imaging of Liver Fibrosis: Current State of the Art,” Radiographics 29, 1615–1635 (2009). [CrossRef] [PubMed]
- C. Aube, F. Oberti, N. Koralin, M.-A. Namour, D. Loisel, M. C. Rousselet, P. Bedossa, H. Rifflet, M. Y. Maga, C. Penneau, C. Caron, and P. Cals, “Ultrasonographic diagnosis of hepatic fibrosis or cirrhosis,” J. Hepatol. 30, 472–478 (1999). [CrossRef] [PubMed]
- K. Ishak, A. Baptista, L. Bianchi, F. Callea, J. De Groote, F. Gudat, H. Denk, V. Desmet, G. Korb, and R. N. MacSween, “Histological grading and staging of chronic hepatitis,” J. Hepatol. 22, 696–699 (1995). [CrossRef] [PubMed]
- L. Gailhouste, Y. Le Grand, C. Odin, D. Guyader, B. Turlin, F. Ezan, Y. Dsille, T. Guilbert, A. Bessard, C. Frmin, N. Theret, and G. Baffet, “Fibrillar Collagen Scoring by Second Harmonic Microscopy as a New Tool in the Assessment of Liver Fibrosis,” J. Hepatol. 52, 398–406 (2010). [CrossRef]
- C. Odin, C. Heichette, D. Chretien, and Y. Le Grand, “Second harmonic microscopy of axonemes,” Opt. Express 17, 9235–9240 (2009). [CrossRef] [PubMed]
- C. Odin, T. Guilbert, A. Alkilani, O. Boryskina, V. Fleury, and Y. Le Grand, “Collagen and myosin characterization by orientation field second harmonic microscopy,” Opt. Express 16, 16151–16165 (2008). [CrossRef] [PubMed]
- C. Odin, Y. Le Grand, A. Renault, L. Gailhouste, and G. Baffet, “Orientation fields of nonlinear biological fibrils by second harmonic generation microscopy,” J. Microsc. 229, 32–38 (2008). [CrossRef]
- A. Y. Hui, C.-T. Liew, M. Y.-Y. Go, A. M.-L. Chim, H. L.-Y. Chan, N. W.-Y. Leung, and J. J.-Y. Sung, “Quantitative assessment of fibrosis in liver biopsies from patients with chronic hepatitis B,” Liver Int. 24, 611–618 (2004). [CrossRef]
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
- A. Y. Hui, C.-T. Liew, M. Y.-Y. Go, A. M.-L. Chim, H. L.-Y. Chan, N. W.-Y. Leung, and J. J.-Y. Sung, “Quantitative assessment of fibrosis in liver biopsies from patients with chronic hepatitis B,” Liver Int. 24, 611–618 (2004). [CrossRef]
- S. W. Chu, S. Y. Chen, G. W. Chern, T. H. Tsai, Y. C. Chen, B. L. Lin, and C. K. Sun, “Studies of χ(2)/χ(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy,” Biophys. J. 86, 3914–3922 (2004). [CrossRef] [PubMed]
- L. I.-K. Lin, “A note on the concordance correlation coefficient,” Biometrics 56, 324–325 (2000).
- L. I.-K. Lin, “A concordance correlation coefficient to evaluate reproducibility,” Biometrics 45, 255–268 (1989). [CrossRef]
- P. J. Campagnola, and L. M. Loew, “Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms,” Nat. Biotechnol. 21, 1356–1360 (2003). [CrossRef]
- C. Aube, F. Oberti, N. Koralin, M.-A. Namour, D. Loisel, M. C. Rousselet, P. Bedossa, H. Rifflet, M. Y. Maga, C. Penneau, C. Caron, and P. Cals, “Ultrasonographic diagnosis of hepatic fibrosis or cirrhosis,” J. Hepatol. 30, 472–478 (1999). [CrossRef] [PubMed]
- S. Psilodimitrakopoulos, D. Artigas, G. Soria, I. Amat-Roldan, A. Planas, and P. Loza-Alvarez, “Quantitative discrimination between endogenousSHG sources in mammalian tissue, based ontheir polarization response,” Opt. Express 17, 10168–10176 (2009). [CrossRef] [PubMed]
- S. Psilodimitrakopoulos, V. Petegnief, G. Soria, I. Amat-Roldan, D. Artigas, A. Planas, and P. Loza-Alvarez, “Estimation of the effective orientation of the SHG source in primary cortical neurons,” Opt. Express 17, 14418–14425 (2009). [CrossRef] [PubMed]
- K. Ishak, A. Baptista, L. Bianchi, F. Callea, J. De Groote, F. Gudat, H. Denk, V. Desmet, G. Korb, and R. N. MacSween, “Histological grading and staging of chronic hepatitis,” J. Hepatol. 22, 696–699 (1995). [CrossRef] [PubMed]
- C. Aube, F. Oberti, N. Koralin, M.-A. Namour, D. Loisel, M. C. Rousselet, P. Bedossa, H. Rifflet, M. Y. Maga, C. Penneau, C. Caron, and P. Cals, “Ultrasonographic diagnosis of hepatic fibrosis or cirrhosis,” J. Hepatol. 30, 472–478 (1999). [CrossRef] [PubMed]
- F. Oberti, E. Valsesia, C. Pilette, M. C. Rousselet, P. Bedossa, C. Aub, Y. Gallois, H. Rifflet, M. Y. Maga, D. Penneau-Fontbonne, and P. Cals, “Noninvasive diagnosis of hepatic fibrosis or cirrhosis,” Gastroenterology 113, 1609–1616 (1997). [CrossRef] [PubMed]
- N. H. Afdhal, A. P. Keaveny, S. B. Cohen, D. P. Nunes, N. Maldonado, M. O’Brien, and P. J. Stone, “Urinary assays for desmosine and hydroxylysylpyridinoline in the detection of cirrhosis,” J. Hepatol. 27, 993–1002 (1997). [CrossRef]
- A.-M. Pena, A. Fabre, D. Dbarre, J. Marchal-Somme, B. Crestani, J.-L. Martin, E. Beaurepaire, and M.-C. Schanne-Klein, “Three-dimensional investigation and scoring of extracellular matrix remodeling during lung fibrosis using multiphoton microscopy,” Microsc. Res. Tech. 70, 162–170 (2007). [CrossRef]
- A.-M. Pena, A. Fabre, D. Dbarre, J. Marchal-Somme, B. Crestani, J.-L. Martin, E. Beaurepaire, and M.-C. Schanne-Klein, “Three-dimensional investigation and scoring of extracellular matrix remodeling during lung fibrosis using multiphoton microscopy,” Microsc. Res. Tech. 70, 162–170 (2007). [CrossRef]
- M. Masseroli, and T. Caballero, “F. OValle, R. M. Del Moral, A. Perez-Milena, and R. G. Del Moral “Automatic quantification of liver fibrosis design and validation of a new image analysis method: comparison with semiquantitative indexes of fibrosis,”,” J. Hepatol. 32, 453–464 (2000). [CrossRef] [PubMed]
- S. C. Faria, K. Ganesan, I. Mwangi, M. Shiehmorteza, B. Viamonte, S. Mazhar, M. Peterson, Y. Kono, C. Santillan, G. Casola, and C. B. Sirlin, “MR Imaging of Liver Fibrosis: Current State of the Art,” Radiographics 29, 1615–1635 (2009). [CrossRef] [PubMed]
- J. Mertz, and L. Moreaux, “Second-harmonic generation by focused excitation of inhomogeneously distributed scatterers,” Opt. Commun. 196, 325–330 (2001). [CrossRef]
- S. V. Plotnikov, A. C. Millard, and P. J. Campagnola, and W. A. Mohler, “Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres,” Biophys. J. 90, 693–703 (2006). [CrossRef]
- M. A. Friedenberg, L. Miller, C. Y. Chung, F. Fleszler, F. L. Banson, R. Thomas, K. P. Swartz, and F. K. Friedenberg, “Simplified method of hepatic fibrosis quantification: design of a new morphometric analysis application,” Liver Int. 25, 1156–1161 (2005). [CrossRef]
- S. V. Plotnikov, A. C. Millard, and P. J. Campagnola, and W. A. Mohler, “Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres,” Biophys. J. 90, 693–703 (2006). [CrossRef]
- J. Mertz, and L. Moreaux, “Second-harmonic generation by focused excitation of inhomogeneously distributed scatterers,” Opt. Commun. 196, 325–330 (2001). [CrossRef]
- S. C. Faria, K. Ganesan, I. Mwangi, M. Shiehmorteza, B. Viamonte, S. Mazhar, M. Peterson, Y. Kono, C. Santillan, G. Casola, and C. B. Sirlin, “MR Imaging of Liver Fibrosis: Current State of the Art,” Radiographics 29, 1615–1635 (2009). [CrossRef] [PubMed]
- C. Aube, F. Oberti, N. Koralin, M.-A. Namour, D. Loisel, M. C. Rousselet, P. Bedossa, H. Rifflet, M. Y. Maga, C. Penneau, C. Caron, and P. Cals, “Ultrasonographic diagnosis of hepatic fibrosis or cirrhosis,” J. Hepatol. 30, 472–478 (1999). [CrossRef] [PubMed]
- W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, “Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation,” Proc. Natl. Acad. Sci. U.S.A. 100, 7075–7080 (2003). [CrossRef] [PubMed]
- N. H. Afdhal, and D. Nunes, “Evaluation of liver fibrosis: a concise review,” Am. J. Gastroenterol. 99, 1160–1174 (2004). [CrossRef] [PubMed]
- N. H. Afdhal, A. P. Keaveny, S. B. Cohen, D. P. Nunes, N. Maldonado, M. O’Brien, and P. J. Stone, “Urinary assays for desmosine and hydroxylysylpyridinoline in the detection of cirrhosis,” J. Hepatol. 27, 993–1002 (1997). [CrossRef]
- N. H. Afdhal, A. P. Keaveny, S. B. Cohen, D. P. Nunes, N. Maldonado, M. O’Brien, and P. J. Stone, “Urinary assays for desmosine and hydroxylysylpyridinoline in the detection of cirrhosis,” J. Hepatol. 27, 993–1002 (1997). [CrossRef]
- C. Aube, F. Oberti, N. Koralin, M.-A. Namour, D. Loisel, M. C. Rousselet, P. Bedossa, H. Rifflet, M. Y. Maga, C. Penneau, C. Caron, and P. Cals, “Ultrasonographic diagnosis of hepatic fibrosis or cirrhosis,” J. Hepatol. 30, 472–478 (1999). [CrossRef] [PubMed]
- F. Oberti, E. Valsesia, C. Pilette, M. C. Rousselet, P. Bedossa, C. Aub, Y. Gallois, H. Rifflet, M. Y. Maga, D. Penneau-Fontbonne, and P. Cals, “Noninvasive diagnosis of hepatic fibrosis or cirrhosis,” Gastroenterology 113, 1609–1616 (1997). [CrossRef] [PubMed]
- L. Gailhouste, Y. Le Grand, C. Odin, D. Guyader, B. Turlin, F. Ezan, Y. Dsille, T. Guilbert, A. Bessard, C. Frmin, N. Theret, and G. Baffet, “Fibrillar Collagen Scoring by Second Harmonic Microscopy as a New Tool in the Assessment of Liver Fibrosis,” J. Hepatol. 52, 398–406 (2010). [CrossRef]
- C. Odin, C. Heichette, D. Chretien, and Y. Le Grand, “Second harmonic microscopy of axonemes,” Opt. Express 17, 9235–9240 (2009). [CrossRef] [PubMed]
- C. Odin, T. Guilbert, A. Alkilani, O. Boryskina, V. Fleury, and Y. Le Grand, “Collagen and myosin characterization by orientation field second harmonic microscopy,” Opt. Express 16, 16151–16165 (2008). [CrossRef] [PubMed]
- C. Odin, Y. Le Grand, A. Renault, L. Gailhouste, and G. Baffet, “Orientation fields of nonlinear biological fibrils by second harmonic generation microscopy,” J. Microsc. 229, 32–38 (2008). [CrossRef]
- P. Bedossa, D. Dargre, and V. Paradis, “Sampling variability of liver fibrosis in chronic hepatitis C,” J. Hepatol. 38, 1356–1358 (2003).
- A.-M. Pena, A. Fabre, D. Dbarre, J. Marchal-Somme, B. Crestani, J.-L. Martin, E. Beaurepaire, and M.-C. Schanne-Klein, “Three-dimensional investigation and scoring of extracellular matrix remodeling during lung fibrosis using multiphoton microscopy,” Microsc. Res. Tech. 70, 162–170 (2007). [CrossRef]
- C. Aube, F. Oberti, N. Koralin, M.-A. Namour, D. Loisel, M. C. Rousselet, P. Bedossa, H. Rifflet, M. Y. Maga, C. Penneau, C. Caron, and P. Cals, “Ultrasonographic diagnosis of hepatic fibrosis or cirrhosis,” J. Hepatol. 30, 472–478 (1999). [CrossRef] [PubMed]
- F. Oberti, E. Valsesia, C. Pilette, M. C. Rousselet, P. Bedossa, C. Aub, Y. Gallois, H. Rifflet, M. Y. Maga, D. Penneau-Fontbonne, and P. Cals, “Noninvasive diagnosis of hepatic fibrosis or cirrhosis,” Gastroenterology 113, 1609–1616 (1997). [CrossRef] [PubMed]
- S. C. Faria, K. Ganesan, I. Mwangi, M. Shiehmorteza, B. Viamonte, S. Mazhar, M. Peterson, Y. Kono, C. Santillan, G. Casola, and C. B. Sirlin, “MR Imaging of Liver Fibrosis: Current State of the Art,” Radiographics 29, 1615–1635 (2009). [CrossRef] [PubMed]
- F. Oberti, E. Valsesia, C. Pilette, M. C. Rousselet, P. Bedossa, C. Aub, Y. Gallois, H. Rifflet, M. Y. Maga, D. Penneau-Fontbonne, and P. Cals, “Noninvasive diagnosis of hepatic fibrosis or cirrhosis,” Gastroenterology 113, 1609–1616 (1997). [CrossRef] [PubMed]
- S. Psilodimitrakopoulos, V. Petegnief, G. Soria, I. Amat-Roldan, D. Artigas, A. Planas, and P. Loza-Alvarez, “Estimation of the effective orientation of the SHG source in primary cortical neurons,” Opt. Express 17, 14418–14425 (2009). [CrossRef] [PubMed]
- S. Psilodimitrakopoulos, D. Artigas, G. Soria, I. Amat-Roldan, A. Planas, and P. Loza-Alvarez, “Quantitative discrimination between endogenousSHG sources in mammalian tissue, based ontheir polarization response,” Opt. Express 17, 10168–10176 (2009). [CrossRef] [PubMed]
- S. V. Plotnikov, A. C. Millard, and P. J. Campagnola, and W. A. Mohler, “Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres,” Biophys. J. 90, 693–703 (2006). [CrossRef]
- S. Psilodimitrakopoulos, V. Petegnief, G. Soria, I. Amat-Roldan, D. Artigas, A. Planas, and P. Loza-Alvarez, “Estimation of the effective orientation of the SHG source in primary cortical neurons,” Opt. Express 17, 14418–14425 (2009). [CrossRef] [PubMed]
- S. Psilodimitrakopoulos, D. Artigas, G. Soria, I. Amat-Roldan, A. Planas, and P. Loza-Alvarez, “Quantitative discrimination between endogenousSHG sources in mammalian tissue, based ontheir polarization response,” Opt. Express 17, 10168–10176 (2009). [CrossRef] [PubMed]
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
- C. Odin, Y. Le Grand, A. Renault, L. Gailhouste, and G. Baffet, “Orientation fields of nonlinear biological fibrils by second harmonic generation microscopy,” J. Microsc. 229, 32–38 (2008). [CrossRef]
- C. Aube, F. Oberti, N. Koralin, M.-A. Namour, D. Loisel, M. C. Rousselet, P. Bedossa, H. Rifflet, M. Y. Maga, C. Penneau, C. Caron, and P. Cals, “Ultrasonographic diagnosis of hepatic fibrosis or cirrhosis,” J. Hepatol. 30, 472–478 (1999). [CrossRef] [PubMed]
- F. Oberti, E. Valsesia, C. Pilette, M. C. Rousselet, P. Bedossa, C. Aub, Y. Gallois, H. Rifflet, M. Y. Maga, D. Penneau-Fontbonne, and P. Cals, “Noninvasive diagnosis of hepatic fibrosis or cirrhosis,” Gastroenterology 113, 1609–1616 (1997). [CrossRef] [PubMed]
- G. L. Davis, J. E. Albright, S. F. Cook, and D. M. Rosenberg, “Projecting future complications of chronic hepatitis C in the United States,” Liver Transpl. 9, 331–338 (2003). [CrossRef] [PubMed]
- S. Roth, and I. Freund, “Second harmonic generation in collagen,” J. Chem. Phys. 70, 1637–1643 (1979). [CrossRef]
- C. Aube, F. Oberti, N. Koralin, M.-A. Namour, D. Loisel, M. C. Rousselet, P. Bedossa, H. Rifflet, M. Y. Maga, C. Penneau, C. Caron, and P. Cals, “Ultrasonographic diagnosis of hepatic fibrosis or cirrhosis,” J. Hepatol. 30, 472–478 (1999). [CrossRef] [PubMed]
- F. Oberti, E. Valsesia, C. Pilette, M. C. Rousselet, P. Bedossa, C. Aub, Y. Gallois, H. Rifflet, M. Y. Maga, D. Penneau-Fontbonne, and P. Cals, “Noninvasive diagnosis of hepatic fibrosis or cirrhosis,” Gastroenterology 113, 1609–1616 (1997). [CrossRef] [PubMed]
- H. A. Saleh, and A. H. Abu-Rashed, “Liver Biopsy Remains the Gold Standard for Evaluation of Chronic Hepatitis and Fibrosis,” J. Gastrointestin. Liver Dis. 16, 425–426 (2007).
- S. C. Faria, K. Ganesan, I. Mwangi, M. Shiehmorteza, B. Viamonte, S. Mazhar, M. Peterson, Y. Kono, C. Santillan, G. Casola, and C. B. Sirlin, “MR Imaging of Liver Fibrosis: Current State of the Art,” Radiographics 29, 1615–1635 (2009). [CrossRef] [PubMed]
- A.-M. Pena, A. Fabre, D. Dbarre, J. Marchal-Somme, B. Crestani, J.-L. Martin, E. Beaurepaire, and M.-C. Schanne-Klein, “Three-dimensional investigation and scoring of extracellular matrix remodeling during lung fibrosis using multiphoton microscopy,” Microsc. Res. Tech. 70, 162–170 (2007). [CrossRef]
- P. J. Scheuer, “Classification of of chronic viral hepatitis: a need for reassessment,” J. Hepatol. 13, 372–374 (1991). [CrossRef] [PubMed]
- S. C. Faria, K. Ganesan, I. Mwangi, M. Shiehmorteza, B. Viamonte, S. Mazhar, M. Peterson, Y. Kono, C. Santillan, G. Casola, and C. B. Sirlin, “MR Imaging of Liver Fibrosis: Current State of the Art,” Radiographics 29, 1615–1635 (2009). [CrossRef] [PubMed]
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
- S. C. Faria, K. Ganesan, I. Mwangi, M. Shiehmorteza, B. Viamonte, S. Mazhar, M. Peterson, Y. Kono, C. Santillan, G. Casola, and C. B. Sirlin, “MR Imaging of Liver Fibrosis: Current State of the Art,” Radiographics 29, 1615–1635 (2009). [CrossRef] [PubMed]
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
- S. Psilodimitrakopoulos, D. Artigas, G. Soria, I. Amat-Roldan, A. Planas, and P. Loza-Alvarez, “Quantitative discrimination between endogenousSHG sources in mammalian tissue, based ontheir polarization response,” Opt. Express 17, 10168–10176 (2009). [CrossRef] [PubMed]
- S. Psilodimitrakopoulos, V. Petegnief, G. Soria, I. Amat-Roldan, D. Artigas, A. Planas, and P. Loza-Alvarez, “Estimation of the effective orientation of the SHG source in primary cortical neurons,” Opt. Express 17, 14418–14425 (2009). [CrossRef] [PubMed]
- N. H. Afdhal, A. P. Keaveny, S. B. Cohen, D. P. Nunes, N. Maldonado, M. O’Brien, and P. J. Stone, “Urinary assays for desmosine and hydroxylysylpyridinoline in the detection of cirrhosis,” J. Hepatol. 27, 993–1002 (1997). [CrossRef]
- S. W. Chu, S. Y. Chen, G. W. Chern, T. H. Tsai, Y. C. Chen, B. L. Lin, and C. K. Sun, “Studies of χ(2)/χ(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy,” Biophys. J. 86, 3914–3922 (2004). [CrossRef] [PubMed]
- A. Y. Hui, C.-T. Liew, M. Y.-Y. Go, A. M.-L. Chim, H. L.-Y. Chan, N. W.-Y. Leung, and J. J.-Y. Sung, “Quantitative assessment of fibrosis in liver biopsies from patients with chronic hepatitis B,” Liver Int. 24, 611–618 (2004). [CrossRef]
- M. A. Friedenberg, L. Miller, C. Y. Chung, F. Fleszler, F. L. Banson, R. Thomas, K. P. Swartz, and F. K. Friedenberg, “Simplified method of hepatic fibrosis quantification: design of a new morphometric analysis application,” Liver Int. 25, 1156–1161 (2005). [CrossRef]
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
- X. Han, R. M. Burke, M. L. Zettel, P. Tang, and E. B. Brown, “Second harmonic properties of tumor collagen: determining the structural relationship between reactive stroma and healthy stroma,” Opt. Express 16, 1846–1859 (2008), http://www.opticsinfobase.org/abstract.cfm?URI=oe-16-3-1846. [CrossRef] [PubMed]
- L. Gailhouste, Y. Le Grand, C. Odin, D. Guyader, B. Turlin, F. Ezan, Y. Dsille, T. Guilbert, A. Bessard, C. Frmin, N. Theret, and G. Baffet, “Fibrillar Collagen Scoring by Second Harmonic Microscopy as a New Tool in the Assessment of Liver Fibrosis,” J. Hepatol. 52, 398–406 (2010). [CrossRef]
- M. A. Friedenberg, L. Miller, C. Y. Chung, F. Fleszler, F. L. Banson, R. Thomas, K. P. Swartz, and F. K. Friedenberg, “Simplified method of hepatic fibrosis quantification: design of a new morphometric analysis application,” Liver Int. 25, 1156–1161 (2005). [CrossRef]
- A. Zoumi, A. Yeh, and B. J. Tromberg, “Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence,” Proc. Natl. Acad. Sci. U.S.A.. 20, 11014–11019 (2002). [CrossRef]
- S. W. Chu, S. Y. Chen, G. W. Chern, T. H. Tsai, Y. C. Chen, B. L. Lin, and C. K. Sun, “Studies of χ(2)/χ(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy,” Biophys. J. 86, 3914–3922 (2004). [CrossRef] [PubMed]
- L. Gailhouste, Y. Le Grand, C. Odin, D. Guyader, B. Turlin, F. Ezan, Y. Dsille, T. Guilbert, A. Bessard, C. Frmin, N. Theret, and G. Baffet, “Fibrillar Collagen Scoring by Second Harmonic Microscopy as a New Tool in the Assessment of Liver Fibrosis,” J. Hepatol. 52, 398–406 (2010). [CrossRef]
- F. Oberti, E. Valsesia, C. Pilette, M. C. Rousselet, P. Bedossa, C. Aub, Y. Gallois, H. Rifflet, M. Y. Maga, D. Penneau-Fontbonne, and P. Cals, “Noninvasive diagnosis of hepatic fibrosis or cirrhosis,” Gastroenterology 113, 1609–1616 (1997). [CrossRef] [PubMed]
- S. C. Faria, K. Ganesan, I. Mwangi, M. Shiehmorteza, B. Viamonte, S. Mazhar, M. Peterson, Y. Kono, C. Santillan, G. Casola, and C. B. Sirlin, “MR Imaging of Liver Fibrosis: Current State of the Art,” Radiographics 29, 1615–1635 (2009). [CrossRef] [PubMed]
- R. M. Williams, W. R. Zipfel, and W. W. Webb, “Interpreting Second-Harmonic Generation Images of Collagen I Fibrils,” Biophys. J. 88, 1377–1386 (2005). [CrossRef]
- W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, “Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation,” Proc. Natl. Acad. Sci. U.S.A. 100, 7075–7080 (2003). [CrossRef] [PubMed]
- W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21, 1369–1377 (2003). [CrossRef]
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
- R. M. Williams, W. R. Zipfel, and W. W. Webb, “Interpreting Second-Harmonic Generation Images of Collagen I Fibrils,” Biophys. J. 88, 1377–1386 (2005). [CrossRef]
- W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, “Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation,” Proc. Natl. Acad. Sci. U.S.A. 100, 7075–7080 (2003). [CrossRef] [PubMed]
- W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21, 1369–1377 (2003). [CrossRef]
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
- A. Zoumi, A. Yeh, and B. J. Tromberg, “Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence,” Proc. Natl. Acad. Sci. U.S.A.. 20, 11014–11019 (2002). [CrossRef]
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
- X. Han, R. M. Burke, M. L. Zettel, P. Tang, and E. B. Brown, “Second harmonic properties of tumor collagen: determining the structural relationship between reactive stroma and healthy stroma,” Opt. Express 16, 1846–1859 (2008), http://www.opticsinfobase.org/abstract.cfm?URI=oe-16-3-1846. [CrossRef] [PubMed]
- R. M. Williams, W. R. Zipfel, and W. W. Webb, “Interpreting Second-Harmonic Generation Images of Collagen I Fibrils,” Biophys. J. 88, 1377–1386 (2005). [CrossRef]
- W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, “Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation,” Proc. Natl. Acad. Sci. U.S.A. 100, 7075–7080 (2003). [CrossRef] [PubMed]
- W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21, 1369–1377 (2003). [CrossRef]
- A. Zoumi, A. Yeh, and B. J. Tromberg, “Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence,” Proc. Natl. Acad. Sci. U.S.A.. 20, 11014–11019 (2002). [CrossRef]
Am. J. Gastroenterol.
- N. H. Afdhal, and D. Nunes, “Evaluation of liver fibrosis: a concise review,” Am. J. Gastroenterol. 99, 1160–1174 (2004). [CrossRef] [PubMed]
Biometrics
- L. I.-K. Lin, “A concordance correlation coefficient to evaluate reproducibility,” Biometrics 45, 255–268 (1989). [CrossRef]
- L. I.-K. Lin, “A note on the concordance correlation coefficient,” Biometrics 56, 324–325 (2000).
Biophys. J.
- S. W. Chu, S. Y. Chen, G. W. Chern, T. H. Tsai, Y. C. Chen, B. L. Lin, and C. K. Sun, “Studies of χ(2)/χ(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy,” Biophys. J. 86, 3914–3922 (2004). [CrossRef] [PubMed]
- R. M. Williams, W. R. Zipfel, and W. W. Webb, “Interpreting Second-Harmonic Generation Images of Collagen I Fibrils,” Biophys. J. 88, 1377–1386 (2005). [CrossRef]
- S. V. Plotnikov, A. C. Millard, and P. J. Campagnola, and W. A. Mohler, “Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres,” Biophys. J. 90, 693–703 (2006). [CrossRef]
Gastroenterology
- F. Oberti, E. Valsesia, C. Pilette, M. C. Rousselet, P. Bedossa, C. Aub, Y. Gallois, H. Rifflet, M. Y. Maga, D. Penneau-Fontbonne, and P. Cals, “Noninvasive diagnosis of hepatic fibrosis or cirrhosis,” Gastroenterology 113, 1609–1616 (1997). [CrossRef] [PubMed]
J. Biomed. Opt.
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
J. Chem. Phys.
- S. Roth, and I. Freund, “Second harmonic generation in collagen,” J. Chem. Phys. 70, 1637–1643 (1979). [CrossRef]
J. Clin. Invest.
- R. Bataller, and D. A. Brenner, “Liver fibrosis,” J. Clin. Invest. 115, 209–218 (2005). [PubMed]
J. Gastrointestin. Liver Dis.
- H. A. Saleh, and A. H. Abu-Rashed, “Liver Biopsy Remains the Gold Standard for Evaluation of Chronic Hepatitis and Fibrosis,” J. Gastrointestin. Liver Dis. 16, 425–426 (2007).
J. Hepatol.
- P. Bedossa, D. Dargre, and V. Paradis, “Sampling variability of liver fibrosis in chronic hepatitis C,” J. Hepatol. 38, 1356–1358 (2003).
- P. J. Scheuer, “Classification of of chronic viral hepatitis: a need for reassessment,” J. Hepatol. 13, 372–374 (1991). [CrossRef] [PubMed]
- K. Ishak, A. Baptista, L. Bianchi, F. Callea, J. De Groote, F. Gudat, H. Denk, V. Desmet, G. Korb, and R. N. MacSween, “Histological grading and staging of chronic hepatitis,” J. Hepatol. 22, 696–699 (1995). [CrossRef] [PubMed]
- The French METAVIR Cooperative Study Group, “Intraobserver, interobserver variations in liver biopsy interpretation in patients with chronic hepatitis C,” J. Hepatol. 20, 15–20 (1995).
- M. Masseroli, and T. Caballero, “F. OValle, R. M. Del Moral, A. Perez-Milena, and R. G. Del Moral “Automatic quantification of liver fibrosis design and validation of a new image analysis method: comparison with semiquantitative indexes of fibrosis,”,” J. Hepatol. 32, 453–464 (2000). [CrossRef] [PubMed]
- S. L. Friedman, “Liver fibrosis - from bench to bedside,” J. Hepatol. 38, 38–53 (2003). [CrossRef]
- N. H. Afdhal, A. P. Keaveny, S. B. Cohen, D. P. Nunes, N. Maldonado, M. O’Brien, and P. J. Stone, “Urinary assays for desmosine and hydroxylysylpyridinoline in the detection of cirrhosis,” J. Hepatol. 27, 993–1002 (1997). [CrossRef]
- C. Aube, F. Oberti, N. Koralin, M.-A. Namour, D. Loisel, M. C. Rousselet, P. Bedossa, H. Rifflet, M. Y. Maga, C. Penneau, C. Caron, and P. Cals, “Ultrasonographic diagnosis of hepatic fibrosis or cirrhosis,” J. Hepatol. 30, 472–478 (1999). [CrossRef] [PubMed]
- L. Gailhouste, Y. Le Grand, C. Odin, D. Guyader, B. Turlin, F. Ezan, Y. Dsille, T. Guilbert, A. Bessard, C. Frmin, N. Theret, and G. Baffet, “Fibrillar Collagen Scoring by Second Harmonic Microscopy as a New Tool in the Assessment of Liver Fibrosis,” J. Hepatol. 52, 398–406 (2010). [CrossRef]
- P. Bedossa, “Harmony in liver fibrosis.,” J. Hepatol. 52, 313–314 (2010). [CrossRef]
J. Microsc.
- C. Odin, Y. Le Grand, A. Renault, L. Gailhouste, and G. Baffet, “Orientation fields of nonlinear biological fibrils by second harmonic generation microscopy,” J. Microsc. 229, 32–38 (2008). [CrossRef]
- K. König, “Multiphoton microscopy in life sciences,” J. Microsc. 200, 83–104 (2000). [CrossRef] [PubMed]
Liver Int.
- A. Y. Hui, C.-T. Liew, M. Y.-Y. Go, A. M.-L. Chim, H. L.-Y. Chan, N. W.-Y. Leung, and J. J.-Y. Sung, “Quantitative assessment of fibrosis in liver biopsies from patients with chronic hepatitis B,” Liver Int. 24, 611–618 (2004). [CrossRef]
- M. A. Friedenberg, L. Miller, C. Y. Chung, F. Fleszler, F. L. Banson, R. Thomas, K. P. Swartz, and F. K. Friedenberg, “Simplified method of hepatic fibrosis quantification: design of a new morphometric analysis application,” Liver Int. 25, 1156–1161 (2005). [CrossRef]
Liver Transpl.
- G. L. Davis, J. E. Albright, S. F. Cook, and D. M. Rosenberg, “Projecting future complications of chronic hepatitis C in the United States,” Liver Transpl. 9, 331–338 (2003). [CrossRef] [PubMed]
Microsc. Res. Tech.
- A.-M. Pena, A. Fabre, D. Dbarre, J. Marchal-Somme, B. Crestani, J.-L. Martin, E. Beaurepaire, and M.-C. Schanne-Klein, “Three-dimensional investigation and scoring of extracellular matrix remodeling during lung fibrosis using multiphoton microscopy,” Microsc. Res. Tech. 70, 162–170 (2007). [CrossRef]
Nat. Biotechnol.
- P. J. Campagnola, and L. M. Loew, “Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms,” Nat. Biotechnol. 21, 1356–1360 (2003). [CrossRef]
- W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21, 1369–1377 (2003). [CrossRef]
Opt. Commun.
- J. Mertz, and L. Moreaux, “Second-harmonic generation by focused excitation of inhomogeneously distributed scatterers,” Opt. Commun. 196, 325–330 (2001). [CrossRef]
Opt. Express
- C. Odin, T. Guilbert, A. Alkilani, O. Boryskina, V. Fleury, and Y. Le Grand, “Collagen and myosin characterization by orientation field second harmonic microscopy,” Opt. Express 16, 16151–16165 (2008). [CrossRef] [PubMed]
- C. Odin, C. Heichette, D. Chretien, and Y. Le Grand, “Second harmonic microscopy of axonemes,” Opt. Express 17, 9235–9240 (2009). [CrossRef] [PubMed]
- S. Psilodimitrakopoulos, D. Artigas, G. Soria, I. Amat-Roldan, A. Planas, and P. Loza-Alvarez, “Quantitative discrimination between endogenousSHG sources in mammalian tissue, based ontheir polarization response,” Opt. Express 17, 10168–10176 (2009). [CrossRef] [PubMed]
- S. Psilodimitrakopoulos, V. Petegnief, G. Soria, I. Amat-Roldan, D. Artigas, A. Planas, and P. Loza-Alvarez, “Estimation of the effective orientation of the SHG source in primary cortical neurons,” Opt. Express 17, 14418–14425 (2009). [CrossRef] [PubMed]
- M. Strupler, A. Pena, M. Hernest, P. Tharaux, J. Martin, E. Beaurepaire, and M. Schanne-Klein, “Second harmonic imaging and scoring of collagen in fibrotic tissues,” Opt. Express 15, 4054–4065 (2007). [CrossRef] [PubMed]
- X. Han, R. M. Burke, M. L. Zettel, P. Tang, and E. B. Brown, “Second harmonic properties of tumor collagen: determining the structural relationship between reactive stroma and healthy stroma,” Opt. Express 16, 1846–1859 (2008), http://www.opticsinfobase.org/abstract.cfm?URI=oe-16-3-1846. [CrossRef] [PubMed]
- H. Bao, A. Boussioutas, R. Jeremy, S. Russell, and M. Gu, “Second harmonic generation imaging via nonlinear endomicroscopy,” Opt. Express 18, 1255–1260 (2010). [CrossRef] [PubMed]
Proc. Natl. Acad. Sci. U.S.A.
- W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, “Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation,” Proc. Natl. Acad. Sci. U.S.A. 100, 7075–7080 (2003). [CrossRef] [PubMed]
Proc. Natl. Acad. Sci. U.S.A..
- A. Zoumi, A. Yeh, and B. J. Tromberg, “Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence,” Proc. Natl. Acad. Sci. U.S.A.. 20, 11014–11019 (2002). [CrossRef]
Radiographics
- S. C. Faria, K. Ganesan, I. Mwangi, M. Shiehmorteza, B. Viamonte, S. Mazhar, M. Peterson, Y. Kono, C. Santillan, G. Casola, and C. B. Sirlin, “MR Imaging of Liver Fibrosis: Current State of the Art,” Radiographics 29, 1615–1635 (2009). [CrossRef] [PubMed]
2010, Gailhouste, J. Hepatol.
- L. Gailhouste, Y. Le Grand, C. Odin, D. Guyader, B. Turlin, F. Ezan, Y. Dsille, T. Guilbert, A. Bessard, C. Frmin, N. Theret, and G. Baffet, “Fibrillar Collagen Scoring by Second Harmonic Microscopy as a New Tool in the Assessment of Liver Fibrosis,” J. Hepatol. 52, 398–406 (2010). [CrossRef]
- P. Bedossa, “Harmony in liver fibrosis.,” J. Hepatol. 52, 313–314 (2010). [CrossRef]
- D. C. S. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. Li Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. C. So, H.-H. Tang, C. Shing Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy,” J. Biomed. Opt. 14(4), 044013 (2009), doi:10.1117/1.3183811. [CrossRef] [PubMed]
- S. C. Faria, K. Ganesan, I. Mwangi, M. Shiehmorteza, B. Viamonte, S. Mazhar, M. Peterson, Y. Kono, C. Santillan, G. Casola, and C. B. Sirlin, “MR Imaging of Liver Fibrosis: Current State of the Art,” Radiographics 29, 1615–1635 (2009). [CrossRef] [PubMed]
- C. Odin, Y. Le Grand, A. Renault, L. Gailhouste, and G. Baffet, “Orientation fields of nonlinear biological fibrils by second harmonic generation microscopy,” J. Microsc. 229, 32–38 (2008). [CrossRef]
- X. Han, R. M. Burke, M. L. Zettel, P. Tang, and E. B. Brown, “Second harmonic properties of tumor collagen: determining the structural relationship between reactive stroma and healthy stroma,” Opt. Express 16, 1846–1859 (2008), http://www.opticsinfobase.org/abstract.cfm?URI=oe-16-3-1846. [CrossRef] [PubMed]
- A.-M. Pena, A. Fabre, D. Dbarre, J. Marchal-Somme, B. Crestani, J.-L. Martin, E. Beaurepaire, and M.-C. Schanne-Klein, “Three-dimensional investigation and scoring of extracellular matrix remodeling during lung fibrosis using multiphoton microscopy,” Microsc. Res. Tech. 70, 162–170 (2007). [CrossRef]
- H. A. Saleh, and A. H. Abu-Rashed, “Liver Biopsy Remains the Gold Standard for Evaluation of Chronic Hepatitis and Fibrosis,” J. Gastrointestin. Liver Dis. 16, 425–426 (2007).
- S. V. Plotnikov, A. C. Millard, and P. J. Campagnola, and W. A. Mohler, “Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres,” Biophys. J. 90, 693–703 (2006). [CrossRef]
- R. Bataller, and D. A. Brenner, “Liver fibrosis,” J. Clin. Invest. 115, 209–218 (2005). [PubMed]
- M. A. Friedenberg, L. Miller, C. Y. Chung, F. Fleszler, F. L. Banson, R. Thomas, K. P. Swartz, and F. K. Friedenberg, “Simplified method of hepatic fibrosis quantification: design of a new morphometric analysis application,” Liver Int. 25, 1156–1161 (2005). [CrossRef]
- R. M. Williams, W. R. Zipfel, and W. W. Webb, “Interpreting Second-Harmonic Generation Images of Collagen I Fibrils,” Biophys. J. 88, 1377–1386 (2005). [CrossRef]
- S. W. Chu, S. Y. Chen, G. W. Chern, T. H. Tsai, Y. C. Chen, B. L. Lin, and C. K. Sun, “Studies of χ(2)/χ(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy,” Biophys. J. 86, 3914–3922 (2004). [CrossRef] [PubMed]
- A. Y. Hui, C.-T. Liew, M. Y.-Y. Go, A. M.-L. Chim, H. L.-Y. Chan, N. W.-Y. Leung, and J. J.-Y. Sung, “Quantitative assessment of fibrosis in liver biopsies from patients with chronic hepatitis B,” Liver Int. 24, 611–618 (2004). [CrossRef]
- N. H. Afdhal, and D. Nunes, “Evaluation of liver fibrosis: a concise review,” Am. J. Gastroenterol. 99, 1160–1174 (2004). [CrossRef] [PubMed]
- G. L. Davis, J. E. Albright, S. F. Cook, and D. M. Rosenberg, “Projecting future complications of chronic hepatitis C in the United States,” Liver Transpl. 9, 331–338 (2003). [CrossRef] [PubMed]
- P. Bedossa, D. Dargre, and V. Paradis, “Sampling variability of liver fibrosis in chronic hepatitis C,” J. Hepatol. 38, 1356–1358 (2003).
- S. L. Friedman, “Liver fibrosis - from bench to bedside,” J. Hepatol. 38, 38–53 (2003). [CrossRef]
- P. J. Campagnola, and L. M. Loew, “Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms,” Nat. Biotechnol. 21, 1356–1360 (2003). [CrossRef]
- W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21, 1369–1377 (2003). [CrossRef]
- W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, “Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation,” Proc. Natl. Acad. Sci. U.S.A. 100, 7075–7080 (2003). [CrossRef] [PubMed]
- A. Zoumi, A. Yeh, and B. J. Tromberg, “Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence,” Proc. Natl. Acad. Sci. U.S.A.. 20, 11014–11019 (2002). [CrossRef]
- J. Mertz, and L. Moreaux, “Second-harmonic generation by focused excitation of inhomogeneously distributed scatterers,” Opt. Commun. 196, 325–330 (2001). [CrossRef]
- K. König, “Multiphoton microscopy in life sciences,” J. Microsc. 200, 83–104 (2000). [CrossRef] [PubMed]
- M. Masseroli, and T. Caballero, “F. OValle, R. M. Del Moral, A. Perez-Milena, and R. G. Del Moral “Automatic quantification of liver fibrosis design and validation of a new image analysis method: comparison with semiquantitative indexes of fibrosis,”,” J. Hepatol. 32, 453–464 (2000). [CrossRef] [PubMed]
- L. I.-K. Lin, “A note on the concordance correlation coefficient,” Biometrics 56, 324–325 (2000).
- C. Aube, F. Oberti, N. Koralin, M.-A. Namour, D. Loisel, M. C. Rousselet, P. Bedossa, H. Rifflet, M. Y. Maga, C. Penneau, C. Caron, and P. Cals, “Ultrasonographic diagnosis of hepatic fibrosis or cirrhosis,” J. Hepatol. 30, 472–478 (1999). [CrossRef] [PubMed]
- F. Oberti, E. Valsesia, C. Pilette, M. C. Rousselet, P. Bedossa, C. Aub, Y. Gallois, H. Rifflet, M. Y. Maga, D. Penneau-Fontbonne, and P. Cals, “Noninvasive diagnosis of hepatic fibrosis or cirrhosis,” Gastroenterology 113, 1609–1616 (1997). [CrossRef] [PubMed]
- N. H. Afdhal, A. P. Keaveny, S. B. Cohen, D. P. Nunes, N. Maldonado, M. O’Brien, and P. J. Stone, “Urinary assays for desmosine and hydroxylysylpyridinoline in the detection of cirrhosis,” J. Hepatol. 27, 993–1002 (1997). [CrossRef]
- K. Ishak, A. Baptista, L. Bianchi, F. Callea, J. De Groote, F. Gudat, H. Denk, V. Desmet, G. Korb, and R. N. MacSween, “Histological grading and staging of chronic hepatitis,” J. Hepatol. 22, 696–699 (1995). [CrossRef] [PubMed]
- The French METAVIR Cooperative Study Group, “Intraobserver, interobserver variations in liver biopsy interpretation in patients with chronic hepatitis C,” J. Hepatol. 20, 15–20 (1995).
- P. J. Scheuer, “Classification of of chronic viral hepatitis: a need for reassessment,” J. Hepatol. 13, 372–374 (1991). [CrossRef] [PubMed]
- L. I.-K. Lin, “A concordance correlation coefficient to evaluate reproducibility,” Biometrics 45, 255–268 (1989). [CrossRef]
- S. Roth, and I. Freund, “Second harmonic generation in collagen,” J. Chem. Phys. 70, 1637–1643 (1979). [CrossRef]
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