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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 25 — Dec. 6, 2010
  • pp: 26469–26474

Simultaneous calibration of optical tweezers spring constant and position detector response

Antoine Le Gall, Karen Perronet, David Dulin, André Villing, Philippe Bouyer, Koen Visscher, and Nathalie Westbrook  »View Author Affiliations

Optics Express, Vol. 18, Issue 25, pp. 26469-26474 (2010)

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We demonstrate a fast and direct calibration method for systems using a single laser for optical tweezers and particle position detection. The method takes direct advantage of back-focal-plane interferometry measuring not an absolute but a differential position, i.e. the position of the trapped particle relative to the center of the optical tweezers. Therefore, a fast step-wise motion of the optical tweezers yields the impulse response of the trapped particle. Calibration parameters such as the detector’s spatial and temporal response and the spring constant of the optical tweezers then follow readily from fitting the measured impulse response.

© 2010 Optical Society of America

OCIS Codes
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Optical Trapping and Manipulation

Original Manuscript: September 20, 2010
Revised Manuscript: November 23, 2010
Manuscript Accepted: November 28, 2010
Published: December 2, 2010

Antoine Le Gall, Karen Perronet, David Dulin, André Villing, Philippe Bouyer, Koen Visscher, and Nathalie Westbrook, "Simultaneous calibration of optical tweezers spring constant and position detector response," Opt. Express 18, 26469-26474 (2010)

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