Opto-electrophoretic detection of bio-molecules using conducting chalcogenide glass sensors

doi:10.1364/OE.18.026754

Opto-electrophoretic detection of bio-molecules using conducting chalcogenide glass sensors

Zhiyong Yang, Megan K. Fah, Kelly A. Reynolds, Jonathan D. Sexton, Mark R. Riley, Marie-Laure Anne, Bruno Bureau, and Pierre Lucas »View Author Affiliations

Optics Express, Vol. 18, Issue 25, pp. 26754-26759 (2010)
http://dx.doi.org/10.1364/OE.18.026754

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Abstract

Novel telluride glasses with high electrical conductivity, wide infrared transparency and good resistance to crystallization are used to design an opto-electrophoretic sensor for detection and identification of hazardous microorganisms. The sensor is based on an attenuated total reflectance element made of Ge-As-Te glass that serves as both an optical sensing zone and an electrode for driving the migration of bio-molecules within the evanescent wave of the sensor. An electric field is applied between the optical element and a counter electrode in order to induce the migration of bio-molecules carrying surface charges. The effect of concentration and applied voltage is tested and the migration effect is shown to be reversible upon switching the electric field. The collected signal is of high quality and can be used to identify different bacterial genus through statistical spectral analysis. This technique therefore provides the ability to detect hazardous microorganisms with high specificity and high sensitivity in aqueous environments. This has great potential for online monitoring of water quality.

OCIS Codes
(160.2750) Materials : Glass and other amorphous materials
(300.6340) Spectroscopy : Spectroscopy, infrared
(280.1415) Remote sensing and sensors : Biological sensing and sensors

ToC Category:
Chalcogenide Glass

History
Original Manuscript: August 27, 2010
Revised Manuscript: October 6, 2010
Manuscript Accepted: October 6, 2010
Published: December 6, 2010

Virtual Issues
Chalcogenide Glass (2010) Optics Express

Citation
Zhiyong Yang, Megan K. Fah, Kelly A. Reynolds, Jonathan D. Sexton, Mark R. Riley, Marie-Laure Anne, Bruno Bureau, and Pierre Lucas, "Opto-electrophoretic detection of bio-molecules using conducting chalcogenide glass sensors," Opt. Express 18, 26754-26759 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-25-26754

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References

D. Naumann, D. Helm, and H. Labischinski, “Microbiological characterizations by FT-IR spectroscopy,” Nature 351(6321), 81–82 (1991). [CrossRef] [PubMed]
M. Diem, S. Boydston-White, and L. Chiriboga, “Infrared Spectroscopy of Cells and Tissues: Shinning Light onto a Novel Subject,” Appl. Spectrosc. 53(4), 148–161 (1999). [CrossRef]
D. Naumann, “Infrared spectroscopy in microbiology,” in Encyclopedia of analytical chemistry, R. A. Meyers, ed. (John Wiley & Sons Ltd, Chichester, 2000), p. 102.
P. Lucas, M. R. Riley, C. Boussard-Plédel, and B. Bureau, “Advances in chalcogenide fiber evanescent wave biochemical sensing,” Anal. Biochem. 351(1), 1–10 (2006). [CrossRef]
Y. Raichlin and A. Katzir, “Fiber-optic evanescent wave spectroscopy in the middle infrared,” Appl. Spectrosc. 62(2), 55–72 (2008). [CrossRef]
B. Mizaikoff, “Mid-IR fiber-optic sensors,” Anal. Chem. 75(11), 258A–267A (2003). [CrossRef] [PubMed]
Z. Yang, A. A. Wilhelm, and P. Lucas, “High-conductivity tellurium-based infrared transmitting glasses and their suitability for bio-optical detection,” J. Am. Ceram. Soc. 93, 1941–1944 (2010).
A. A. Wilhelm, P. Lucas, D. L. DeRosa, and M. R. Riley, “Biocompatibility of Te–As–Se glass fibers for cell-based bio-optic infrared sensors,” J. Mater. Res. 22(4), 1098–1104 (2007). [CrossRef]
P. Lucas, D. Le Coq, C. Juncker, J. Collier, D. E. Boesewetter, C. Boussard-Plédel, B. Bureau, and M. R. Riley, “Evaluation of toxic agent effects on lung cells by fiber evanescent wave spectroscopy,” Appl. Spectrosc. 59(1), 1–9 (2005). [CrossRef] [PubMed]
P. G. Righetti and T. Caravaggio, “Isoelectric points and molecular weights of proteins: A table,” J. Chromatogr. A 127(1), 1–28 (1976). [CrossRef]
H. Miörner, P. A. Albertsson, and G. Kronvall, “Isoelectric points and surface hydrophobicity of Gram-positive cocci as determined by cross-partition and hydrophobic affinity partition in aqueous two-phase systems,” Infect. Immun. 36(1), 227–234 (1982). [PubMed]
J. Keirsse, B. Bureau, C. Boussard-Pledel, P. Leroyer, M. Ropert, V. Dupont, M. L. Anne, C. Ribault, O. Sire, O. Loreal, and J. L. Adam, “Chalcogenide glass fibers for in-situ infrared spectroscopy in biology and medicine,” Proc. SPIE 5459, 61–68 (2004). [CrossRef]

Anal. Biochem.

P. Lucas, M. R. Riley, C. Boussard-Plédel, and B. Bureau, “Advances in chalcogenide fiber evanescent wave biochemical sensing,” Anal. Biochem. 351(1), 1–10 (2006). [CrossRef]

Anal. Chem.

B. Mizaikoff, “Mid-IR fiber-optic sensors,” Anal. Chem. 75(11), 258A–267A (2003). [CrossRef] [PubMed]

Appl. Spectrosc.

P. Lucas, D. Le Coq, C. Juncker, J. Collier, D. E. Boesewetter, C. Boussard-Plédel, B. Bureau, and M. R. Riley, “Evaluation of toxic agent effects on lung cells by fiber evanescent wave spectroscopy,” Appl. Spectrosc. 59(1), 1–9 (2005). [CrossRef] [PubMed]
Y. Raichlin and A. Katzir, “Fiber-optic evanescent wave spectroscopy in the middle infrared,” Appl. Spectrosc. 62(2), 55–72 (2008). [CrossRef]
M. Diem, S. Boydston-White, and L. Chiriboga, “Infrared Spectroscopy of Cells and Tissues: Shinning Light onto a Novel Subject,” Appl. Spectrosc. 53(4), 148–161 (1999). [CrossRef]

Infect. Immun.

H. Miörner, P. A. Albertsson, and G. Kronvall, “Isoelectric points and surface hydrophobicity of Gram-positive cocci as determined by cross-partition and hydrophobic affinity partition in aqueous two-phase systems,” Infect. Immun. 36(1), 227–234 (1982). [PubMed]

J. Am. Ceram. Soc.

Z. Yang, A. A. Wilhelm, and P. Lucas, “High-conductivity tellurium-based infrared transmitting glasses and their suitability for bio-optical detection,” J. Am. Ceram. Soc. 93, 1941–1944 (2010).

J. Chromatogr. A

P. G. Righetti and T. Caravaggio, “Isoelectric points and molecular weights of proteins: A table,” J. Chromatogr. A 127(1), 1–28 (1976). [CrossRef]

J. Mater. Res.

A. A. Wilhelm, P. Lucas, D. L. DeRosa, and M. R. Riley, “Biocompatibility of Te–As–Se glass fibers for cell-based bio-optic infrared sensors,” J. Mater. Res. 22(4), 1098–1104 (2007). [CrossRef]

Nature

D. Naumann, D. Helm, and H. Labischinski, “Microbiological characterizations by FT-IR spectroscopy,” Nature 351(6321), 81–82 (1991). [CrossRef] [PubMed]

Proc. SPIE

J. Keirsse, B. Bureau, C. Boussard-Pledel, P. Leroyer, M. Ropert, V. Dupont, M. L. Anne, C. Ribault, O. Sire, O. Loreal, and J. L. Adam, “Chalcogenide glass fibers for in-situ infrared spectroscopy in biology and medicine,” Proc. SPIE 5459, 61–68 (2004). [CrossRef]

Other

D. Naumann, “Infrared spectroscopy in microbiology,” in Encyclopedia of analytical chemistry, R. A. Meyers, ed. (John Wiley & Sons Ltd, Chichester, 2000), p. 102.

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