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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 26 — Dec. 20, 2010
  • pp: 26905–26914

Two-photon spectral imaging with high temporal and spectral resolution

Kang-Bin Im, Moon-Sik Kang, Jiho Kim, Felix Bestvater, Zahir Seghiri, Malte Wachsmuth, and Regis Grailhe  »View Author Affiliations


Optics Express, Vol. 18, Issue 26, pp. 26905-26914 (2010)
http://dx.doi.org/10.1364/OE.18.026905


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Abstract

We introduce a fast spectral imaging system using an electron-multiplying charge-coupled device (EM-CCD) as a detector. Our system is combined with a custom-built two-photon excitation laser scanning microscope and has 80 detection channels, which allow for high spectral resolution and fast frame acquisition without any loss of spectral information. To demonstrate the efficiency of our approach, we applied this technology to monitor fluorescent proteins and quantum dot-labeled G protein-coupled receptors in living cells as well as autofluorescence in tissue samples.

© 2010 OSA

OCIS Codes
(040.1520) Detectors : CCD, charge-coupled device
(300.6410) Spectroscopy : Spectroscopy, multiphoton
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Spectroscopy

History
Original Manuscript: October 27, 2010
Revised Manuscript: November 27, 2010
Manuscript Accepted: November 30, 2010
Published: December 7, 2010

Citation
Kang-Bin Im, Moon-Sik Kang, Jiho Kim, Felix Bestvater, Zahir Seghiri, Malte Wachsmuth, and Regis Grailhe, "Two-photon spectral imaging with high temporal and spectral resolution," Opt. Express 18, 26905-26914 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-26-26905


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