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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 26 — Dec. 20, 2010
  • pp: 27431–27444

Terahertz and far infrared Spectroscopy of alanine-rich peptides having variable ellipticity

Tao Ding, Ruoyu Li, J. Axel Zeitler, Thomas L. Huber, Lynn F. Gladden, Anton P. J. Middelberg, and Robert J. Falconer  »View Author Affiliations

Optics Express, Vol. 18, Issue 26, pp. 27431-27444 (2010)

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Terahertz spectra of four alanine-rich peptides with known secondary structures were studied by terahertz time domain spectroscopy (THz-TDS) and by Fourier transform infrared spectroscopy (FTIR) using a synchrotron light source and a liquid-helium cooled bolometer. At ambient temperatures the usable bandwidth was restricted to 0.2-1.5 THz by the absorbance of water. The existence of a solvation shell around the peptide in solution was observed and its size estimated to be between 11 and 17 Å. By cooling the peptide solution to 80 K in order to reduce the water absorbance the bandwidth was increased to 0.1-3.0 THz for both THz-TDS and FTIR. Spectra were consistent with monotonic absorbance of the peptide and the existence of a solid amorphous low density solvation shell.

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OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(300.6495) Spectroscopy : Spectroscopy, teraherz

ToC Category:

Original Manuscript: October 27, 2010
Revised Manuscript: December 8, 2010
Manuscript Accepted: December 8, 2010
Published: December 14, 2010

Tao Ding, Ruoyu Li, J. Axel Zeitler, Thomas L. Huber, Lynn F. Gladden, Anton P. J. Middelberg, and Robert J. Falconer, "Terahertz and far infrared Spectroscopy of alanine-rich peptides having variable ellipticity," Opt. Express 18, 27431-27444 (2010)

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