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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 3 — Feb. 1, 2010
  • pp: 2858–2871

Diffusive and directional intracellular dynamics measured by field-based dynamic light scattering

Chulmin Joo, Conor L. Evans, Thomas Stepinac, Tayyaba Hasan, and Johannes F. de Boer  »View Author Affiliations


Optics Express, Vol. 18, Issue 3, pp. 2858-2871 (2010)
http://dx.doi.org/10.1364/OE.18.002858


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Abstract

Quantitative measurement of diffusive and directional processes of intracellular structures is not only critical in understanding cell mechanics and functions, but also has many applications, such as investigation of cellular responses to therapeutic agents. We introduce a label-free optical technique that allows non-perturbative characterization of localized intracellular dynamics. The method combines a field-based dynamic light scattering analysis with a confocal interferometric microscope to provide a statistical measure of the diffusive and directional motion of scattering structures inside a microscopic probe volume. To demonstrate the potential of this technique, we examined the localized intracellular dynamics in human epithelial ovarian cancer cells. We observed the distinctive temporal regimes of intracellular dynamics, which transitions from random to directional processes on a timescale of ~0.01 sec. In addition, we observed disrupted directional processes on the timescale of 1~5 sec by the application of a microtubule polymerization inhibitor, Colchicine, and ATP depletion.

© 2010 OSA

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(290.1350) Scattering : Backscattering
(290.5820) Scattering : Scattering measurements

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: October 21, 2009
Revised Manuscript: December 17, 2009
Manuscript Accepted: December 29, 2009
Published: January 27, 2010

Virtual Issues
Vol. 5, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Chulmin Joo, Conor L. Evans, Thomas Stepinac, Tayyaba Hasan, and Johannes F. de Boer, "Diffusive and directional intracellular dynamics measured by field-based dynamic light scattering," Opt. Express 18, 2858-2871 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-3-2858


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