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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 3 — Feb. 1, 2010
  • pp: 3023–3034

Depth of field multiplexing in microscopy

Christian Maurer, Saranjam Khan, Stephanie Fassl, Stefan Bernet, and Monika Ritsch-Marte  »View Author Affiliations

Optics Express, Vol. 18, Issue 3, pp. 3023-3034 (2010)

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We demonstrate “depth of field multiplexing” by a high resolution spatial light modulator (SLM) in a Fourier plane in the imaging path of a standard microscope. This approach provides simultaneous imaging of different focal planes in a sample with only a single camera exposure. The phase mask on the SLM corresponds to a set of superposed multi-focal off-axis Fresnel lenses, which sharply image different focal planes of the object to non-overlapping adjacent sections of the camera chip. Depth of field multiplexing allows to record motion in a three dimensional sample volume in real-time, which is exemplarily demonstrated for cytoplasmic streaming in plant cells and rapidly swimming protozoa.

© 2010 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.6880) Imaging systems : Three-dimensional image acquisition
(230.3720) Optical devices : Liquid-crystal devices

ToC Category:

Original Manuscript: November 25, 2009
Revised Manuscript: January 15, 2010
Manuscript Accepted: January 22, 2010
Published: January 28, 2010

Virtual Issues
Vol. 5, Iss. 4 Virtual Journal for Biomedical Optics

Christian Maurer, Saranjam Khan, Stephanie Fassl, Stefan Bernet, and Monika Ritsch-Marte, "Depth of field multiplexing in microscopy," Opt. Express 18, 3023-3034 (2010)

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