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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 4 — Feb. 15, 2010
  • pp: 3322–3331

Superresolution imaging in optical tweezers using high-speed cameras

Juan Pablo Staforelli, Esteban Vera, José Manuel Brito, Pablo Solano, Sergio Torres, and Carlos Saavedra  »View Author Affiliations

Optics Express, Vol. 18, Issue 4, pp. 3322-3331 (2010)

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High-speed cameras are reliable alternatives for the direct characterization of optical trap force and particle motion in optical tweezers setups, replacing indirect motion measurements often performed by quadrant detectors. In the present approach, subpixel motion data of the trapped particle is retrieved from a high-speed low-resolution video sequence. Due to the richness structure of motion diversity of microscopic trapped particles, which are subjected to a Brownian motion, we propose to also use the obtained motion information for tackling the inherent lack of resolution by applying superresolution algorithms on the low-resolution image sequence. The obtained results both for trapping calibration beads and for living bacteria show that the proposed approach allows the proper characterization of the optical tweezers by obtaining the real particle motion directly from the image domain, while still providing high resolution imaging.

© 2010 OSA

OCIS Codes
(100.6640) Image processing : Superresolution
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation

ToC Category:
Optical Trapping and Manipulation

Original Manuscript: December 11, 2009
Revised Manuscript: January 22, 2010
Manuscript Accepted: January 22, 2010
Published: February 1, 2010

Virtual Issues
Vol. 5, Iss. 5 Virtual Journal for Biomedical Optics

Juan Pablo Staforelli, Esteban Vera, José Manuel Brito, Pablo Solano, Sergio Torres, and Carlos Saavedra, "Superresolution imaging in optical tweezers using high-speed cameras," Opt. Express 18, 3322-3331 (2010)

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