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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 5 — Mar. 1, 2010
  • pp: 4717–4726

On-chip differential interference contrast microscopy using lensless digital holography

Chulwoo Oh, Serhan O. Isikman, Bahar Khademhosseinieh, and Aydogan Ozcan  »View Author Affiliations

Optics Express, Vol. 18, Issue 5, pp. 4717-4726 (2010)

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We introduce the use of a birefringent crystal with lensless digital holography to create an on-chip differential interference contrast (DIC) microscope. Using an incoherent source with a large aperture, in-line holograms of micro-objects are created, which interact with a uniaxial crystal and an absorbing polarizer, encoding differential interference contrast information of the objects on the chip. Despite the fact that a unit fringe magnification and an incoherent source with a large aperture have been used, holographic digital processing of such holograms rapidly recovers the differential phase contrast image of the specimen over a large field-of-view of ~24 mm2.

© 2010 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.3170) Microscopy : Interference microscopy
(090.1995) Holography : Digital holography

ToC Category:

Original Manuscript: January 11, 2010
Revised Manuscript: February 6, 2010
Manuscript Accepted: February 9, 2010
Published: February 22, 2010

Virtual Issues
Vol. 5, Iss. 6 Virtual Journal for Biomedical Optics

Chulwoo Oh, Serhan O. Isikman, Bahar Khademhosseinieh, and Aydogan Ozcan, "On-chip differential interference contrast microscopy using lensless digital holography," Opt. Express 18, 4717-4726 (2010)

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